Metabolomic analysis by UAE-GC MS and antioxidant activity of Salvia hispanica (L.) seeds grown under different irrigation regimes

Bruna de Falco, Alberto Fiore, Rocco Bochicchio, Mariana Amato, Virginia Lanzotti

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    Abstract

    Chia (Salvia hispanica L.) is an emerging crop with a high content of α-linolenic acid and metabolites of industrial and pharmaceutical interest but information on metabolome variations in response to agricultural management is scarce. We investigated the yield and metabolic profile of the seeds of two chia populations, one commercial black (B) and one long-day flowering genotype (G8), in response to two irrigation levels: replacement of 100% ET⁠0 (I) or rainfed (NI). Seed yield was higher in irrigated plots in G8 only (0.255kgm⁠−2 for I vs 0.184kgm⁠−2 for NI) while it was very low regardless of irrigation in B due to late flowering. Ultrasound assisted extraction (UAE) of seeds followed by gas chromatography-mass spectrometry (GC/MS) analysis showed differences in fatty acids and the major classes of organic compounds due to both genotype and irrigation, especially in the non-polar phase where irrigated samples showed a higher content of α-linolenic and other fatty acids and a lower oleic/linoleic ratio (47.4 in NI vs. 39.6 in I). The antioxidant activity, expressed as trolox equivalent antioxidant capacity (TEAC), ranged from 1.317±0.027 to 2.174±0.010mmol TEAC/g of defatted chia seed after 2 and 40min respectively, and was negatively affected by irrigation. The total polyphenolic content (TPC) measured with the Folin-Ciocalteu method, also decreased with irrigation. According to our results irrigation can affect chia yield, metabolome and antioxidant behavior but some of the effects are genotype-dependent.
    Original languageEnglish
    Pages (from-to)584–592
    Number of pages9
    JournalIndustrial Crops and Products
    Volume112
    Early online date4 Jan 2018
    DOIs
    Publication statusPublished - Feb 2018

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    Salvia hispanica
    metabolomics
    irrigation management
    antioxidant activity
    irrigation
    metabolome
    seeds
    antioxidants
    genotype
    fatty acids
    flowering
    agricultural management
    irrigation rates
    linolenic acid
    organic compounds
    seed yield
    polyphenols
    metabolites
    drugs
    crops

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    @article{2c9dd41c3bf0447fa155ab570f41a992,
    title = "Metabolomic analysis by UAE-GC MS and antioxidant activity of Salvia hispanica (L.) seeds grown under different irrigation regimes",
    abstract = "Chia (Salvia hispanica L.) is an emerging crop with a high content of α-linolenic acid and metabolites of industrial and pharmaceutical interest but information on metabolome variations in response to agricultural management is scarce. We investigated the yield and metabolic profile of the seeds of two chia populations, one commercial black (B) and one long-day flowering genotype (G8), in response to two irrigation levels: replacement of 100{\%} ET⁠0 (I) or rainfed (NI). Seed yield was higher in irrigated plots in G8 only (0.255kgm⁠−2 for I vs 0.184kgm⁠−2 for NI) while it was very low regardless of irrigation in B due to late flowering. Ultrasound assisted extraction (UAE) of seeds followed by gas chromatography-mass spectrometry (GC/MS) analysis showed differences in fatty acids and the major classes of organic compounds due to both genotype and irrigation, especially in the non-polar phase where irrigated samples showed a higher content of α-linolenic and other fatty acids and a lower oleic/linoleic ratio (47.4 in NI vs. 39.6 in I). The antioxidant activity, expressed as trolox equivalent antioxidant capacity (TEAC), ranged from 1.317±0.027 to 2.174±0.010mmol TEAC/g of defatted chia seed after 2 and 40min respectively, and was negatively affected by irrigation. The total polyphenolic content (TPC) measured with the Folin-Ciocalteu method, also decreased with irrigation. According to our results irrigation can affect chia yield, metabolome and antioxidant behavior but some of the effects are genotype-dependent.",
    author = "{de Falco}, Bruna and Alberto Fiore and Rocco Bochicchio and Mariana Amato and Virginia Lanzotti",
    year = "2018",
    month = "2",
    doi = "10.1016/j.indcrop.2017.12.030",
    language = "English",
    volume = "112",
    pages = "584–592",
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    }

    Metabolomic analysis by UAE-GC MS and antioxidant activity of Salvia hispanica (L.) seeds grown under different irrigation regimes. / de Falco, Bruna; Fiore, Alberto; Bochicchio, Rocco; Amato, Mariana; Lanzotti, Virginia.

    In: Industrial Crops and Products, Vol. 112, 02.2018, p. 584–592.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Metabolomic analysis by UAE-GC MS and antioxidant activity of Salvia hispanica (L.) seeds grown under different irrigation regimes

    AU - de Falco, Bruna

    AU - Fiore, Alberto

    AU - Bochicchio, Rocco

    AU - Amato, Mariana

    AU - Lanzotti, Virginia

    PY - 2018/2

    Y1 - 2018/2

    N2 - Chia (Salvia hispanica L.) is an emerging crop with a high content of α-linolenic acid and metabolites of industrial and pharmaceutical interest but information on metabolome variations in response to agricultural management is scarce. We investigated the yield and metabolic profile of the seeds of two chia populations, one commercial black (B) and one long-day flowering genotype (G8), in response to two irrigation levels: replacement of 100% ET⁠0 (I) or rainfed (NI). Seed yield was higher in irrigated plots in G8 only (0.255kgm⁠−2 for I vs 0.184kgm⁠−2 for NI) while it was very low regardless of irrigation in B due to late flowering. Ultrasound assisted extraction (UAE) of seeds followed by gas chromatography-mass spectrometry (GC/MS) analysis showed differences in fatty acids and the major classes of organic compounds due to both genotype and irrigation, especially in the non-polar phase where irrigated samples showed a higher content of α-linolenic and other fatty acids and a lower oleic/linoleic ratio (47.4 in NI vs. 39.6 in I). The antioxidant activity, expressed as trolox equivalent antioxidant capacity (TEAC), ranged from 1.317±0.027 to 2.174±0.010mmol TEAC/g of defatted chia seed after 2 and 40min respectively, and was negatively affected by irrigation. The total polyphenolic content (TPC) measured with the Folin-Ciocalteu method, also decreased with irrigation. According to our results irrigation can affect chia yield, metabolome and antioxidant behavior but some of the effects are genotype-dependent.

    AB - Chia (Salvia hispanica L.) is an emerging crop with a high content of α-linolenic acid and metabolites of industrial and pharmaceutical interest but information on metabolome variations in response to agricultural management is scarce. We investigated the yield and metabolic profile of the seeds of two chia populations, one commercial black (B) and one long-day flowering genotype (G8), in response to two irrigation levels: replacement of 100% ET⁠0 (I) or rainfed (NI). Seed yield was higher in irrigated plots in G8 only (0.255kgm⁠−2 for I vs 0.184kgm⁠−2 for NI) while it was very low regardless of irrigation in B due to late flowering. Ultrasound assisted extraction (UAE) of seeds followed by gas chromatography-mass spectrometry (GC/MS) analysis showed differences in fatty acids and the major classes of organic compounds due to both genotype and irrigation, especially in the non-polar phase where irrigated samples showed a higher content of α-linolenic and other fatty acids and a lower oleic/linoleic ratio (47.4 in NI vs. 39.6 in I). The antioxidant activity, expressed as trolox equivalent antioxidant capacity (TEAC), ranged from 1.317±0.027 to 2.174±0.010mmol TEAC/g of defatted chia seed after 2 and 40min respectively, and was negatively affected by irrigation. The total polyphenolic content (TPC) measured with the Folin-Ciocalteu method, also decreased with irrigation. According to our results irrigation can affect chia yield, metabolome and antioxidant behavior but some of the effects are genotype-dependent.

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    DO - 10.1016/j.indcrop.2017.12.030

    M3 - Article

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    JO - Industrial Crops and Products

    JF - Industrial Crops and Products

    SN - 0926-6690

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