Metabolomics approach based on NMR spectroscopy and multivariate data analysis to explore the interaction between the leafminer Tuta absoluta and tomato (Solanum lycopersicum)

Bruna de Falco, Daniele Manzo, Guido Incerti, Antonio Pietro Garonna, Maria Ercolano*, Virginia Lanzotti

*Corresponding author for this work

    Research output: Contribution to journalSpecial issue

    Abstract

    Introduction: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most devastating and harmful pests of tomato (Solanum lycopersicum) crops causing up to 80–100% yield losses. A large arsenal of plant metabolites is induced by the leafminer feeding including defence compounds that could differ among varieties. 

    Objective: To compare the metabolomic changes of different genotypes of tomato (tolerant “T”, susceptible “S” and “F1” hybrid obtained between T and S) after exposition to T. absoluta. 

    Methodology: Nuclear magnetic resonance (NMR) spectroscopy followed by multivariate data analysis were performed to analyse the metabolic profiles of control and infested samples on three different tomato genotypes.

    Results: Signals related to GABA (γ-aminobutyric acid) were relatively much higher in all infested samples compared to the non-infested plants used as control. Infested T genotype samples were the most abundant in organic acids, including fatty acids and acyl sugars, chlorogenic acid, neo-chlorogenic acid and feruloyl quinic acid, indicating a clear link between the exposure to leafminer. Results also showed an increase of trigonelline in all tomato varieties after exposition to T. absoluta

    Conclusion: Metabolomics approach based on NMR spectroscopy followed by multivariate data analysis allowed for a detailed metabolite profile of plant defences, providing fundamental information for breeding programmes in plant crops.

    Original languageEnglish
    Pages (from-to)556-563
    Number of pages8
    JournalPhytochemical Analysis
    Volume30
    Issue number5
    Early online date8 Jul 2019
    DOIs
    Publication statusE-pub ahead of print - 8 Jul 2019

    Fingerprint

    Tuta absoluta
    Chlorogenic Acid
    Metabolomics
    leafminers
    Solanum lycopersicum
    metabolomics
    Lycopersicon esculentum
    Metabolites
    Nuclear magnetic resonance spectroscopy
    multivariate analysis
    Crops
    nuclear magnetic resonance spectroscopy
    Magnetic Resonance Spectroscopy
    Multivariate Analysis
    tomatoes
    Arsenals
    Aminobutyrates
    Organic acids
    chlorogenic acid
    gamma-Aminobutyric Acid

    Cite this

    de Falco, Bruna ; Manzo, Daniele ; Incerti, Guido ; Garonna, Antonio Pietro ; Ercolano, Maria ; Lanzotti, Virginia. / Metabolomics approach based on NMR spectroscopy and multivariate data analysis to explore the interaction between the leafminer Tuta absoluta and tomato (Solanum lycopersicum). In: Phytochemical Analysis. 2019 ; Vol. 30, No. 5. pp. 556-563.
    @article{38e6b83571804c329411e6a98b621b9c,
    title = "Metabolomics approach based on NMR spectroscopy and multivariate data analysis to explore the interaction between the leafminer Tuta absoluta and tomato (Solanum lycopersicum)",
    abstract = "Introduction: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most devastating and harmful pests of tomato (Solanum lycopersicum) crops causing up to 80–100{\%} yield losses. A large arsenal of plant metabolites is induced by the leafminer feeding including defence compounds that could differ among varieties. Objective: To compare the metabolomic changes of different genotypes of tomato (tolerant “T”, susceptible “S” and “F1” hybrid obtained between T and S) after exposition to T. absoluta. Methodology: Nuclear magnetic resonance (NMR) spectroscopy followed by multivariate data analysis were performed to analyse the metabolic profiles of control and infested samples on three different tomato genotypes.Results: Signals related to GABA (γ-aminobutyric acid) were relatively much higher in all infested samples compared to the non-infested plants used as control. Infested T genotype samples were the most abundant in organic acids, including fatty acids and acyl sugars, chlorogenic acid, neo-chlorogenic acid and feruloyl quinic acid, indicating a clear link between the exposure to leafminer. Results also showed an increase of trigonelline in all tomato varieties after exposition to T. absoluta. Conclusion: Metabolomics approach based on NMR spectroscopy followed by multivariate data analysis allowed for a detailed metabolite profile of plant defences, providing fundamental information for breeding programmes in plant crops.",
    author = "{de Falco}, Bruna and Daniele Manzo and Guido Incerti and Garonna, {Antonio Pietro} and Maria Ercolano and Virginia Lanzotti",
    year = "2019",
    month = "7",
    day = "8",
    doi = "10.1002/pca.2850",
    language = "English",
    volume = "30",
    pages = "556--563",
    journal = "Phytochemical Analysis",
    issn = "0958-0344",
    publisher = "John Wiley and Sons Ltd",
    number = "5",

    }

    Metabolomics approach based on NMR spectroscopy and multivariate data analysis to explore the interaction between the leafminer Tuta absoluta and tomato (Solanum lycopersicum). / de Falco, Bruna; Manzo, Daniele; Incerti, Guido; Garonna, Antonio Pietro; Ercolano, Maria; Lanzotti, Virginia.

    In: Phytochemical Analysis, Vol. 30, No. 5, 08.07.2019, p. 556-563.

    Research output: Contribution to journalSpecial issue

    TY - JOUR

    T1 - Metabolomics approach based on NMR spectroscopy and multivariate data analysis to explore the interaction between the leafminer Tuta absoluta and tomato (Solanum lycopersicum)

    AU - de Falco, Bruna

    AU - Manzo, Daniele

    AU - Incerti, Guido

    AU - Garonna, Antonio Pietro

    AU - Ercolano, Maria

    AU - Lanzotti, Virginia

    PY - 2019/7/8

    Y1 - 2019/7/8

    N2 - Introduction: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most devastating and harmful pests of tomato (Solanum lycopersicum) crops causing up to 80–100% yield losses. A large arsenal of plant metabolites is induced by the leafminer feeding including defence compounds that could differ among varieties. Objective: To compare the metabolomic changes of different genotypes of tomato (tolerant “T”, susceptible “S” and “F1” hybrid obtained between T and S) after exposition to T. absoluta. Methodology: Nuclear magnetic resonance (NMR) spectroscopy followed by multivariate data analysis were performed to analyse the metabolic profiles of control and infested samples on three different tomato genotypes.Results: Signals related to GABA (γ-aminobutyric acid) were relatively much higher in all infested samples compared to the non-infested plants used as control. Infested T genotype samples were the most abundant in organic acids, including fatty acids and acyl sugars, chlorogenic acid, neo-chlorogenic acid and feruloyl quinic acid, indicating a clear link between the exposure to leafminer. Results also showed an increase of trigonelline in all tomato varieties after exposition to T. absoluta. Conclusion: Metabolomics approach based on NMR spectroscopy followed by multivariate data analysis allowed for a detailed metabolite profile of plant defences, providing fundamental information for breeding programmes in plant crops.

    AB - Introduction: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most devastating and harmful pests of tomato (Solanum lycopersicum) crops causing up to 80–100% yield losses. A large arsenal of plant metabolites is induced by the leafminer feeding including defence compounds that could differ among varieties. Objective: To compare the metabolomic changes of different genotypes of tomato (tolerant “T”, susceptible “S” and “F1” hybrid obtained between T and S) after exposition to T. absoluta. Methodology: Nuclear magnetic resonance (NMR) spectroscopy followed by multivariate data analysis were performed to analyse the metabolic profiles of control and infested samples on three different tomato genotypes.Results: Signals related to GABA (γ-aminobutyric acid) were relatively much higher in all infested samples compared to the non-infested plants used as control. Infested T genotype samples were the most abundant in organic acids, including fatty acids and acyl sugars, chlorogenic acid, neo-chlorogenic acid and feruloyl quinic acid, indicating a clear link between the exposure to leafminer. Results also showed an increase of trigonelline in all tomato varieties after exposition to T. absoluta. Conclusion: Metabolomics approach based on NMR spectroscopy followed by multivariate data analysis allowed for a detailed metabolite profile of plant defences, providing fundamental information for breeding programmes in plant crops.

    U2 - 10.1002/pca.2850

    DO - 10.1002/pca.2850

    M3 - Special issue

    VL - 30

    SP - 556

    EP - 563

    JO - Phytochemical Analysis

    JF - Phytochemical Analysis

    SN - 0958-0344

    IS - 5

    ER -