Molecular estimation of alteration in intestinal microbial composition in Hashimoto's thyroiditis patients

Hafiz Muhammad Ishaq, Imran Shair Mohammad, Hui Guo, Muhammad Shahzad, Yin Jian Hou, Chaofeng Ma, Zahid Naseem, Xiaokang Wu, Peijie Shi, Jiru Xu*

*Corresponding author for this work

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    Abstract

    The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto’s thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism.
    Original languageEnglish
    Pages (from-to)865-874
    Number of pages10
    JournalBiomedicine and Pharmacotherapy
    Volume95
    Early online date10 Sep 2017
    DOIs
    Publication statusPublished - Nov 2017

    Fingerprint

    Hashimoto Disease
    rRNA Genes
    Dysbiosis
    Prevotella
    Escherichia
    Bacteroides
    Bifidobacterium
    Shigella
    Clostridium
    Lactobacillus
    Real-Time Polymerase Chain Reaction
    Escherichia coli
    Polymerase Chain Reaction

    Cite this

    Ishaq, Hafiz Muhammad ; Mohammad, Imran Shair ; Guo, Hui ; Shahzad, Muhammad ; Hou, Yin Jian ; Ma, Chaofeng ; Naseem, Zahid ; Wu, Xiaokang ; Shi, Peijie ; Xu, Jiru. / Molecular estimation of alteration in intestinal microbial composition in Hashimoto's thyroiditis patients. In: Biomedicine and Pharmacotherapy. 2017 ; Vol. 95. pp. 865-874.
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    abstract = "The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto’s thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism.",
    author = "Ishaq, {Hafiz Muhammad} and Mohammad, {Imran Shair} and Hui Guo and Muhammad Shahzad and Hou, {Yin Jian} and Chaofeng Ma and Zahid Naseem and Xiaokang Wu and Peijie Shi and Jiru Xu",
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    Ishaq, HM, Mohammad, IS, Guo, H, Shahzad, M, Hou, YJ, Ma, C, Naseem, Z, Wu, X, Shi, P & Xu, J 2017, 'Molecular estimation of alteration in intestinal microbial composition in Hashimoto's thyroiditis patients', Biomedicine and Pharmacotherapy, vol. 95, pp. 865-874. https://doi.org/10.1016/j.biopha.2017.08.101

    Molecular estimation of alteration in intestinal microbial composition in Hashimoto's thyroiditis patients. / Ishaq, Hafiz Muhammad; Mohammad, Imran Shair; Guo, Hui; Shahzad, Muhammad; Hou, Yin Jian; Ma, Chaofeng; Naseem, Zahid; Wu, Xiaokang; Shi, Peijie; Xu, Jiru.

    In: Biomedicine and Pharmacotherapy, Vol. 95, 11.2017, p. 865-874.

    Research output: Contribution to journalArticle

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    AU - Ishaq, Hafiz Muhammad

    AU - Mohammad, Imran Shair

    AU - Guo, Hui

    AU - Shahzad, Muhammad

    AU - Hou, Yin Jian

    AU - Ma, Chaofeng

    AU - Naseem, Zahid

    AU - Wu, Xiaokang

    AU - Shi, Peijie

    AU - Xu, Jiru

    PY - 2017/11

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    N2 - The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto’s thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism.

    AB - The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto’s thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism.

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