Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 865-874 |
| Number of pages | 10 |
| Journal | Biomedicine and Pharmacotherapy |
| Volume | 95 |
| Early online date | 10 Sep 2017 |
| DOIs | |
| Publication status | Published - Nov 2017 |
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Molecular estimation of alteration in intestinal microbial composition in Hashimoto's thyroiditis patients. / Ishaq, Hafiz Muhammad; Mohammad, Imran Shair; Guo, Hui; Shahzad, Muhammad; Hou, Yin Jian; Ma, Chaofeng; Naseem, Zahid; Wu, Xiaokang; Shi, Peijie; Xu, Jiru.
In: Biomedicine and Pharmacotherapy, Vol. 95, 11.2017, p. 865-874.Research output: Contribution to journal › Article
TY - JOUR
T1 - Molecular estimation of alteration in intestinal microbial composition in Hashimoto's thyroiditis patients
AU - Ishaq, Hafiz Muhammad
AU - Mohammad, Imran Shair
AU - Guo, Hui
AU - Shahzad, Muhammad
AU - Hou, Yin Jian
AU - Ma, Chaofeng
AU - Naseem, Zahid
AU - Wu, Xiaokang
AU - Shi, Peijie
AU - Xu, Jiru
PY - 2017/11
Y1 - 2017/11
N2 - The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto’s thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism.
AB - The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto’s thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism.
U2 - 10.1016/j.biopha.2017.08.101
DO - 10.1016/j.biopha.2017.08.101
M3 - Article
VL - 95
SP - 865
EP - 874
JO - Biomedicine and Pharmacotherapy
JF - Biomedicine and Pharmacotherapy
SN - 0753-3322
ER -