Monoclonal antibody against a nuclear matrix antigen in proliferating human cells

R. N. Philipova*, N. Z. Zhelev, I. T. Todorov, A. A. Hadjiolov

*Corresponding author for this work

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18 Citations (Scopus)

Abstract

A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human cell lines and phytohemagglutinin‐stimulated lymphocytes, but absence in resting lymphocytes; (b) appearance in stimulated lymphocytes in parallel with the onset of DNA synthesis; (c) a speckled distribution in the nucleoplasm; (d) tight association with nuclear matrix structures identified by both biochemical and in situ extraction and enzyme treatment procedures; (e) mol wt of 125 kDa and pI 6.5 as determined by immunoprecipitation or immunoblotting of nuclear or nuclear matrix proteins fractionated by gel electrophoresis. The above characteristics identify the p125/6.5 nuclear matrix protein recognized by the isolated monoclonal antibody as belonging to the class of proliferating cell nuclear antigens. 1987 Société Française des Microscopies and Société Biologie Cellulaire de France

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBiology of the Cell
Volume60
Issue number1
DOIs
Publication statusPublished - 1987
Externally publishedYes

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