Monoclonal antibody against a nuclear matrix antigen in proliferating human cells

R. N. Philipova*, N. Z. Zhelev, I. T. Todorov, A. A. Hadjiolov

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human cell lines and phytohemagglutinin‐stimulated lymphocytes, but absence in resting lymphocytes; (b) appearance in stimulated lymphocytes in parallel with the onset of DNA synthesis; (c) a speckled distribution in the nucleoplasm; (d) tight association with nuclear matrix structures identified by both biochemical and in situ extraction and enzyme treatment procedures; (e) mol wt of 125 kDa and pI 6.5 as determined by immunoprecipitation or immunoblotting of nuclear or nuclear matrix proteins fractionated by gel electrophoresis. The above characteristics identify the p125/6.5 nuclear matrix protein recognized by the isolated monoclonal antibody as belonging to the class of proliferating cell nuclear antigens. 1987 Société Française des Microscopies and Société Biologie Cellulaire de France

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBiology of the Cell
Volume60
Issue number1
DOIs
Publication statusPublished - 1987
Externally publishedYes

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Nuclear Matrix
Nuclear Antigens
Nuclear Matrix-Associated Proteins
Monoclonal Antibodies
Lymphocytes
Hybridomas
Proliferating Cell Nuclear Antigen
Immunoprecipitation
Immunoblotting
France
Electrophoresis
Microscopy
Gels
Cell Line
Peptides
Antibodies
DNA
Enzymes

Cite this

Philipova, R. N. ; Zhelev, N. Z. ; Todorov, I. T. ; Hadjiolov, A. A. / Monoclonal antibody against a nuclear matrix antigen in proliferating human cells. In: Biology of the Cell. 1987 ; Vol. 60, No. 1. pp. 1-8.
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abstract = "A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human cell lines and phytohemagglutinin‐stimulated lymphocytes, but absence in resting lymphocytes; (b) appearance in stimulated lymphocytes in parallel with the onset of DNA synthesis; (c) a speckled distribution in the nucleoplasm; (d) tight association with nuclear matrix structures identified by both biochemical and in situ extraction and enzyme treatment procedures; (e) mol wt of 125 kDa and pI 6.5 as determined by immunoprecipitation or immunoblotting of nuclear or nuclear matrix proteins fractionated by gel electrophoresis. The above characteristics identify the p125/6.5 nuclear matrix protein recognized by the isolated monoclonal antibody as belonging to the class of proliferating cell nuclear antigens. 1987 Soci{\'e}t{\'e} Fran{\cc}aise des Microscopies and Soci{\'e}t{\'e} Biologie Cellulaire de France",
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Monoclonal antibody against a nuclear matrix antigen in proliferating human cells. / Philipova, R. N.; Zhelev, N. Z.; Todorov, I. T.; Hadjiolov, A. A.

In: Biology of the Cell, Vol. 60, No. 1, 1987, p. 1-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Monoclonal antibody against a nuclear matrix antigen in proliferating human cells

AU - Philipova, R. N.

AU - Zhelev, N. Z.

AU - Todorov, I. T.

AU - Hadjiolov, A. A.

PY - 1987

Y1 - 1987

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AB - A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human cell lines and phytohemagglutinin‐stimulated lymphocytes, but absence in resting lymphocytes; (b) appearance in stimulated lymphocytes in parallel with the onset of DNA synthesis; (c) a speckled distribution in the nucleoplasm; (d) tight association with nuclear matrix structures identified by both biochemical and in situ extraction and enzyme treatment procedures; (e) mol wt of 125 kDa and pI 6.5 as determined by immunoprecipitation or immunoblotting of nuclear or nuclear matrix proteins fractionated by gel electrophoresis. The above characteristics identify the p125/6.5 nuclear matrix protein recognized by the isolated monoclonal antibody as belonging to the class of proliferating cell nuclear antigens. 1987 Société Française des Microscopies and Société Biologie Cellulaire de France

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