Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 547-555 |
| Number of pages | 9 |
| Journal | Korean Journal of Microbiology and Biotechnology |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2009 |
Fingerprint
Cite this
}
Nanoscopic morphological changes in yeast cell surfaces caused by oxidative stress : an atomic force microscopic study. / Canetta, Elisabetta; Walker, Graeme M.; Adya, Ashok K.
In: Korean Journal of Microbiology and Biotechnology, Vol. 19, No. 6, 2009, p. 547-555.Research output: Contribution to journal › Article
TY - JOUR
T1 - Nanoscopic morphological changes in yeast cell surfaces caused by oxidative stress
T2 - Microbiology and Biotechnology Letters
AU - Canetta,Elisabetta
AU - Walker,Graeme M.
AU - Adya,Ashok K.
PY - 2009
Y1 - 2009
N2 - Nanoscopic changes in the cell surface morphology of the yeasts Saccharomyces cerevisiae (strain NCYC 1681) and Schizosaccharomyces pombe (strain DVPB 1354), due to their exposure to varying concentrations of hydrogen peroxide (oxidative stress), were investigated using an atomic force microscope (AFM). Increasing hydrogen peroxide concentration led to a decrease in cell viabilities and mean cell volumes, and an increase in the surface roughness of the yeasts. In addition, AFM studies revealed that oxidative stress caused cell compression in both S. cerevisiae and Schiz. pombe cells and an increase in the number of aged yeasts. These results confirmed the importance and usefulness of AFM in investigating the morphology of stressed microbial cells at the nanoscale. The results also provided novel information on the relative oxidative stress tolerance of S. cerevisiae and Schiz. pombe.
AB - Nanoscopic changes in the cell surface morphology of the yeasts Saccharomyces cerevisiae (strain NCYC 1681) and Schizosaccharomyces pombe (strain DVPB 1354), due to their exposure to varying concentrations of hydrogen peroxide (oxidative stress), were investigated using an atomic force microscope (AFM). Increasing hydrogen peroxide concentration led to a decrease in cell viabilities and mean cell volumes, and an increase in the surface roughness of the yeasts. In addition, AFM studies revealed that oxidative stress caused cell compression in both S. cerevisiae and Schiz. pombe cells and an increase in the number of aged yeasts. These results confirmed the importance and usefulness of AFM in investigating the morphology of stressed microbial cells at the nanoscale. The results also provided novel information on the relative oxidative stress tolerance of S. cerevisiae and Schiz. pombe.
U2 - 10.4014/jmb.0809.515
DO - 10.4014/jmb.0809.515
M3 - Article
VL - 19
SP - 547
EP - 555
JO - Microbiology and Biotechnology Letters
JF - Microbiology and Biotechnology Letters
SN - 1598-642X
IS - 6
ER -