Neferine prevents NF-κB translocation and protects muscle cells from oxidative stress and apoptosis induced by hypoxia

Rathinasamy Baskaran, Paramasivan Poornima, Chih Yang Huang, Viswanadha Vijaya Padma

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Neferine (Nef), a bisbenzylisoquinoline alkaloid from lotus seed embryo has a wide range of pharmacological activities. Possible molecular mechanism for the cytoprotective action of Nef during hypoxic stress has not been explored till now. Hence, this is an attempt to elucidate the molecular mechanism involved in the Nef mediated cytoprotection on hypoxia-induced cell injury. Cytoprotective dose of Nef in muscle cells (Human rhabdomyosarcoma cells) exposed to hypoxia was determined by MTT assay. Nef at 500 nM offered better cytoprotection and was used for all the experiments. ROS, intracellular calcium accumulation and mitochondrial membrane (ΔψM) potential were measured using fluorescent probes. Further, we evaluated the effect Nef on hypoxia induced inflammatory and apoptotic responses by FACS and analyzing the expression patterns of NF-κB, COX-2, HIF-1α, caspase-3, caspase-9, Bcl2, and Bax. The results of this study revealed that pretreatment of the cells with Nef significantly decreased the ΔψM and ROS in the cells subjected to hypoxia. Further, Nef inhibited NF-κB there by inhibiting the expression of its downstream regulator COX-2, while it induced the functional HIF-1α expression. The results also indicate that Nef significantly inhibited the ROS dependent mitochondrial mediated apoptosis induced during hypoxia. The cytoprotection elicited by Nef in a model of hypoxia induced cell death involves both anti-inflammatory and anti-apoptotic response. These results suggest that Nef may be used as prophylactic agent against the hypoxic challenge. © 2016 BioFactors, 42(4):407-417, 2016.

Original languageEnglish
Pages (from-to)407-417
Number of pages11
JournalBioFactors
Volume42
Issue number4
Early online date4 Apr 2016
DOIs
Publication statusPublished - 16 Aug 2016
Externally publishedYes

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neferine
Oxidative stress
Muscle Cells
Muscle
Oxidative Stress
Cells
Apoptosis
Cytoprotection
Hypoxia
Benzylisoquinolines

Cite this

Baskaran, Rathinasamy ; Poornima, Paramasivan ; Huang, Chih Yang ; Padma, Viswanadha Vijaya. / Neferine prevents NF-κB translocation and protects muscle cells from oxidative stress and apoptosis induced by hypoxia. In: BioFactors. 2016 ; Vol. 42, No. 4. pp. 407-417.
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Neferine prevents NF-κB translocation and protects muscle cells from oxidative stress and apoptosis induced by hypoxia. / Baskaran, Rathinasamy; Poornima, Paramasivan; Huang, Chih Yang; Padma, Viswanadha Vijaya.

In: BioFactors, Vol. 42, No. 4, 16.08.2016, p. 407-417.

Research output: Contribution to journalArticle

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AU - Poornima, Paramasivan

AU - Huang, Chih Yang

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