No effect of creatine supplementation on human myofibrillar and sarcoplasmic protein synthesis after resistance exercise

Magali Louis, Jacques R. Poortmans, Marc Francaux, Jacques Berré, Nathalie Boisseau, Eric Brassine, Daniel J.R. Cuthbertson, Kenneth Smith, John A. Babraj, Tom Waddell, Michael J. Rennie

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Muscle hypertrophy during resistance training is reportedly increased by creatine supplementation. Having previously failed to find an anabolic effect on muscle protein turnover at rest, either fed or fasted, we have now examined the possibility of a stimulatory effect of creatine in conjunction with acute resistance exercise. Seven healthy men (body mass index, 23 ±2 kg/m2, 21 ± 1 yr, means ± SE) performed 20 x 10 repetitions of leg extension-flexion at 75% one-repetition maximum in one leg, on two occasions, 4 wk apart, before and after ingesting 21 g/day creatine for 5 days. The subjects ate ∼21 g maltodextrin + 6 g protein/h for 3 h postexercise. We measured incorporation of [1-13C]leucine into quadriceps muscle proteins in the rested and exercised legs. Leg protein breakdown (as dilution of [2H5]phenylalanine) was also assessed in the exercised and rested leg postexercise. Creatine supplementation increased muscle total creatine by approximately 21% (P < 0.01). Exercise increased the synthetic rates of myofibrillar and sarcoplasmic proteins by two- to threefold (P < 0.05), and leg phenylalanine balance became more positive, but creatine was without any anabolic effect.
Original languageEnglish
Pages (from-to)E1089-E1094
Number of pages6
JournalAJP Endocrinology & Metabolism
Volume285
Issue number5
DOIs
Publication statusPublished - Nov 2003
Externally publishedYes

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Creatine
Leg
Exercise
Anabolic Agents
Proteins
Muscle Proteins
Phenylalanine
Muscles
Resistance Training
Quadriceps Muscle
Leucine
Hypertrophy
Body Mass Index

Cite this

Louis, M., Poortmans, J. R., Francaux, M., Berré, J., Boisseau, N., Brassine, E., ... Rennie, M. J. (2003). No effect of creatine supplementation on human myofibrillar and sarcoplasmic protein synthesis after resistance exercise. AJP Endocrinology & Metabolism, 285(5), E1089-E1094. https://doi.org/10.1152/ajpendo.00195.2003
Louis, Magali ; Poortmans, Jacques R. ; Francaux, Marc ; Berré, Jacques ; Boisseau, Nathalie ; Brassine, Eric ; Cuthbertson, Daniel J.R. ; Smith, Kenneth ; Babraj, John A. ; Waddell, Tom ; Rennie, Michael J. / No effect of creatine supplementation on human myofibrillar and sarcoplasmic protein synthesis after resistance exercise. In: AJP Endocrinology & Metabolism. 2003 ; Vol. 285, No. 5. pp. E1089-E1094.
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Louis, M, Poortmans, JR, Francaux, M, Berré, J, Boisseau, N, Brassine, E, Cuthbertson, DJR, Smith, K, Babraj, JA, Waddell, T & Rennie, MJ 2003, 'No effect of creatine supplementation on human myofibrillar and sarcoplasmic protein synthesis after resistance exercise', AJP Endocrinology & Metabolism, vol. 285, no. 5, pp. E1089-E1094. https://doi.org/10.1152/ajpendo.00195.2003

No effect of creatine supplementation on human myofibrillar and sarcoplasmic protein synthesis after resistance exercise. / Louis, Magali; Poortmans, Jacques R.; Francaux, Marc; Berré, Jacques; Boisseau, Nathalie; Brassine, Eric; Cuthbertson, Daniel J.R.; Smith, Kenneth; Babraj, John A.; Waddell, Tom; Rennie, Michael J.

In: AJP Endocrinology & Metabolism, Vol. 285, No. 5, 11.2003, p. E1089-E1094.

Research output: Contribution to journalArticle

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T1 - No effect of creatine supplementation on human myofibrillar and sarcoplasmic protein synthesis after resistance exercise

AU - Louis, Magali

AU - Poortmans, Jacques R.

AU - Francaux, Marc

AU - Berré, Jacques

AU - Boisseau, Nathalie

AU - Brassine, Eric

AU - Cuthbertson, Daniel J.R.

AU - Smith, Kenneth

AU - Babraj, John A.

AU - Waddell, Tom

AU - Rennie, Michael J.

PY - 2003/11

Y1 - 2003/11

N2 - Muscle hypertrophy during resistance training is reportedly increased by creatine supplementation. Having previously failed to find an anabolic effect on muscle protein turnover at rest, either fed or fasted, we have now examined the possibility of a stimulatory effect of creatine in conjunction with acute resistance exercise. Seven healthy men (body mass index, 23 ±2 kg/m2, 21 ± 1 yr, means ± SE) performed 20 x 10 repetitions of leg extension-flexion at 75% one-repetition maximum in one leg, on two occasions, 4 wk apart, before and after ingesting 21 g/day creatine for 5 days. The subjects ate ∼21 g maltodextrin + 6 g protein/h for 3 h postexercise. We measured incorporation of [1-13C]leucine into quadriceps muscle proteins in the rested and exercised legs. Leg protein breakdown (as dilution of [2H5]phenylalanine) was also assessed in the exercised and rested leg postexercise. Creatine supplementation increased muscle total creatine by approximately 21% (P < 0.01). Exercise increased the synthetic rates of myofibrillar and sarcoplasmic proteins by two- to threefold (P < 0.05), and leg phenylalanine balance became more positive, but creatine was without any anabolic effect.

AB - Muscle hypertrophy during resistance training is reportedly increased by creatine supplementation. Having previously failed to find an anabolic effect on muscle protein turnover at rest, either fed or fasted, we have now examined the possibility of a stimulatory effect of creatine in conjunction with acute resistance exercise. Seven healthy men (body mass index, 23 ±2 kg/m2, 21 ± 1 yr, means ± SE) performed 20 x 10 repetitions of leg extension-flexion at 75% one-repetition maximum in one leg, on two occasions, 4 wk apart, before and after ingesting 21 g/day creatine for 5 days. The subjects ate ∼21 g maltodextrin + 6 g protein/h for 3 h postexercise. We measured incorporation of [1-13C]leucine into quadriceps muscle proteins in the rested and exercised legs. Leg protein breakdown (as dilution of [2H5]phenylalanine) was also assessed in the exercised and rested leg postexercise. Creatine supplementation increased muscle total creatine by approximately 21% (P < 0.01). Exercise increased the synthetic rates of myofibrillar and sarcoplasmic proteins by two- to threefold (P < 0.05), and leg phenylalanine balance became more positive, but creatine was without any anabolic effect.

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DO - 10.1152/ajpendo.00195.2003

M3 - Article

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SP - E1089-E1094

JO - AJP Endocrinology & Metabolism

JF - AJP Endocrinology & Metabolism

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ER -