No effect of menstrual cycle on myofibrillar and connective tissue protein synthesis in contracting skeletal muscle

Benjamin F. Miller, Mette Hansen, Jens L. Olesen, Allan Flyvbjerg, Peter Schwarz, John A. Babraj, Kenneth Smith, Michael J. Rennie, Michael Kjaer

Research output: Contribution to journalArticle

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Abstract

We tested the hypothesis that acute exercise would stimulate synthesis of myofibrillar protein and intramuscular collagen in women and that the phase of the menstrual cycle at which the exercise took place would influence the extent of the change. Fifteen young, healthy female subjects were studied in the follicular (FP, n=8) or the luteal phase (LP, n=7, n=1 out of phase) 24 h after an acute bout of one-legged exercise (60 min of kicking at 67% Wmax), samples being taken from the vastus lateralis in both the exercised and resting legs. Rates of synthesis of myofibrillar and muscle collagen proteins were measured by incorporation of [13C]leucine. Myofibrillar protein synthesis (means ± SD; rest FP: 0.053 ± 0.009%/h, LP: 0.055 ± 0.013%/h) was increased at 24-h postexercise (FP: 0.131 ± 0.018%/h, P<0.05, LP: 0.134+/-0.018%/h, P< 0.05) with no differences between phases. Similarly, muscle collagen synthesis (rest FP: 0.024 ± 0.017%/h, LP: 0.021± 0.006%/h) was elevated at 24-h postexercise (FP: 0.073± 0.016%/h, P<0.05, LP: 0.072± 0.015%/h, P<0.05), but the responses did not differ between menstrual phases. Therefore, there is no effect of menstrual cycle phase, at rest or in response to an acute bout of exercise, on myofibrillar protein synthesis and muscle collagen synthesis in women.
Original languageEnglish
Pages (from-to)E163-E168
Number of pages6
JournalAJP Endocrinology & Metabolism
Volume290
Issue number1
DOIs
Publication statusPublished - 1 Jan 2006
Externally publishedYes

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Menstrual Cycle
Connective Tissue
Skeletal Muscle
Collagen
Exercise
Muscle Proteins
Proteins
Luteal Phase
Quadriceps Muscle
Leucine
Leg
Healthy Volunteers
Muscles

Cite this

Miller, Benjamin F. ; Hansen, Mette ; Olesen, Jens L. ; Flyvbjerg, Allan ; Schwarz, Peter ; Babraj, John A. ; Smith, Kenneth ; Rennie, Michael J. ; Kjaer, Michael. / No effect of menstrual cycle on myofibrillar and connective tissue protein synthesis in contracting skeletal muscle. In: AJP Endocrinology & Metabolism. 2006 ; Vol. 290, No. 1. pp. E163-E168.
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abstract = "We tested the hypothesis that acute exercise would stimulate synthesis of myofibrillar protein and intramuscular collagen in women and that the phase of the menstrual cycle at which the exercise took place would influence the extent of the change. Fifteen young, healthy female subjects were studied in the follicular (FP, n=8) or the luteal phase (LP, n=7, n=1 out of phase) 24 h after an acute bout of one-legged exercise (60 min of kicking at 67{\%} Wmax), samples being taken from the vastus lateralis in both the exercised and resting legs. Rates of synthesis of myofibrillar and muscle collagen proteins were measured by incorporation of [13C]leucine. Myofibrillar protein synthesis (means ± SD; rest FP: 0.053 ± 0.009{\%}/h, LP: 0.055 ± 0.013{\%}/h) was increased at 24-h postexercise (FP: 0.131 ± 0.018{\%}/h, P<0.05, LP: 0.134+/-0.018{\%}/h, P< 0.05) with no differences between phases. Similarly, muscle collagen synthesis (rest FP: 0.024 ± 0.017{\%}/h, LP: 0.021± 0.006{\%}/h) was elevated at 24-h postexercise (FP: 0.073± 0.016{\%}/h, P<0.05, LP: 0.072± 0.015{\%}/h, P<0.05), but the responses did not differ between menstrual phases. Therefore, there is no effect of menstrual cycle phase, at rest or in response to an acute bout of exercise, on myofibrillar protein synthesis and muscle collagen synthesis in women.",
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Miller, BF, Hansen, M, Olesen, JL, Flyvbjerg, A, Schwarz, P, Babraj, JA, Smith, K, Rennie, MJ & Kjaer, M 2006, 'No effect of menstrual cycle on myofibrillar and connective tissue protein synthesis in contracting skeletal muscle', AJP Endocrinology & Metabolism, vol. 290, no. 1, pp. E163-E168. https://doi.org/10.1152/ajpendo.00300.2005

No effect of menstrual cycle on myofibrillar and connective tissue protein synthesis in contracting skeletal muscle. / Miller, Benjamin F.; Hansen, Mette; Olesen, Jens L.; Flyvbjerg, Allan; Schwarz, Peter; Babraj, John A.; Smith, Kenneth; Rennie, Michael J.; Kjaer, Michael.

In: AJP Endocrinology & Metabolism, Vol. 290, No. 1, 01.01.2006, p. E163-E168.

Research output: Contribution to journalArticle

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AB - We tested the hypothesis that acute exercise would stimulate synthesis of myofibrillar protein and intramuscular collagen in women and that the phase of the menstrual cycle at which the exercise took place would influence the extent of the change. Fifteen young, healthy female subjects were studied in the follicular (FP, n=8) or the luteal phase (LP, n=7, n=1 out of phase) 24 h after an acute bout of one-legged exercise (60 min of kicking at 67% Wmax), samples being taken from the vastus lateralis in both the exercised and resting legs. Rates of synthesis of myofibrillar and muscle collagen proteins were measured by incorporation of [13C]leucine. Myofibrillar protein synthesis (means ± SD; rest FP: 0.053 ± 0.009%/h, LP: 0.055 ± 0.013%/h) was increased at 24-h postexercise (FP: 0.131 ± 0.018%/h, P<0.05, LP: 0.134+/-0.018%/h, P< 0.05) with no differences between phases. Similarly, muscle collagen synthesis (rest FP: 0.024 ± 0.017%/h, LP: 0.021± 0.006%/h) was elevated at 24-h postexercise (FP: 0.073± 0.016%/h, P<0.05, LP: 0.072± 0.015%/h, P<0.05), but the responses did not differ between menstrual phases. Therefore, there is no effect of menstrual cycle phase, at rest or in response to an acute bout of exercise, on myofibrillar protein synthesis and muscle collagen synthesis in women.

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DO - 10.1152/ajpendo.00300.2005

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