Optimizing a sustainable ultrasound assisted extraction method for the recovery of polyphenols from lemon by-products

comparison with hot water and organic solvent extractions

Konstantinos Papoutsis, Penta Pristijono, John B. Golding, Costas E. Stathopoulos, Michael C. Bowyer, Christopher J. Scarlett, Quan V. Vuong

    Research output: Contribution to journalArticle

    7 Citations (Scopus)
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    Abstract

    Response surface methodology (RSM) based on a three-factor and three-level Box–Behnken design was employed for optimizing the aqueous ultrasound-assisted extraction (AUAE) conditions, including extraction time (35–45 min), extraction temperature (45–55 °C) and ultrasonic power (150–250 W), for the recovery of total phenolic content (TPC) and rutin from lemon by-products. The independent variables and their values were selected on the basis of preliminary experiments, where the effects of five extraction parameters (particle size, extraction time and temperature, ultrasonic power and sample-to-solvent ratio) on TPC and rutin extraction yields were investigated. The yields of TPC and rutin were studied using a second-order polynomial equation. The optimum AUAE conditions for TPC were extraction time of 45 min, extraction temperature of 50 °C and ultrasonic power of 250 W with a predicted value of 18.10 ± 0.24 mg GAE/g dw, while the optimum AUAE conditions for rutin were extraction time of 35 min, extraction temperature of 48 °C and ultrasonic power of 150W with a predicted value of 3.20 ± 0.12 mg/g dw. The extracts obtained at the optimum AUAE conditions were compared with those obtained by a hot water and an organic solvent conventional extraction in terms of TPC, total flavonoid content (TF) and antioxidant capacity. The extracts obtained by AUAE had the same TPC, TF and ferric reducing antioxidant power as those achieved by organic solvent conventional extraction. However, hot water extraction led to extracts with the highest flavonoid content and antioxidant capacity. Scanning electron microscopy analysis showed that all the extraction methods led to cell damage to varying extents.
    Original languageEnglish
    Pages (from-to)1353-1365
    Number of pages13
    JournalEuropean Food Research and Technology
    Volume244
    Issue number8
    Early online date19 Feb 2018
    DOIs
    Publication statusPublished - Aug 2018

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    Rutin
    Polyphenols
    lemons
    Solvent extraction
    Ultrasonics
    Organic solvents
    byproducts
    Byproducts
    polyphenols
    Flavonoids
    Recovery
    Temperature
    Water
    Antioxidants
    water
    methodology
    Particle Size
    Electron Scanning Microscopy
    rutin
    ultrasonics

    Cite this

    Papoutsis, Konstantinos ; Pristijono, Penta ; Golding, John B. ; Stathopoulos, Costas E. ; Bowyer, Michael C. ; Scarlett, Christopher J. ; Vuong, Quan V. . / Optimizing a sustainable ultrasound assisted extraction method for the recovery of polyphenols from lemon by-products : comparison with hot water and organic solvent extractions. In: European Food Research and Technology. 2018 ; Vol. 244, No. 8. pp. 1353-1365.
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    title = "Optimizing a sustainable ultrasound assisted extraction method for the recovery of polyphenols from lemon by-products: comparison with hot water and organic solvent extractions",
    abstract = "Response surface methodology (RSM) based on a three-factor and three-level Box–Behnken design was employed for optimizing the aqueous ultrasound-assisted extraction (AUAE) conditions, including extraction time (35–45 min), extraction temperature (45–55 °C) and ultrasonic power (150–250 W), for the recovery of total phenolic content (TPC) and rutin from lemon by-products. The independent variables and their values were selected on the basis of preliminary experiments, where the effects of five extraction parameters (particle size, extraction time and temperature, ultrasonic power and sample-to-solvent ratio) on TPC and rutin extraction yields were investigated. The yields of TPC and rutin were studied using a second-order polynomial equation. The optimum AUAE conditions for TPC were extraction time of 45 min, extraction temperature of 50 °C and ultrasonic power of 250 W with a predicted value of 18.10 ± 0.24 mg GAE/g dw, while the optimum AUAE conditions for rutin were extraction time of 35 min, extraction temperature of 48 °C and ultrasonic power of 150W with a predicted value of 3.20 ± 0.12 mg/g dw. The extracts obtained at the optimum AUAE conditions were compared with those obtained by a hot water and an organic solvent conventional extraction in terms of TPC, total flavonoid content (TF) and antioxidant capacity. The extracts obtained by AUAE had the same TPC, TF and ferric reducing antioxidant power as those achieved by organic solvent conventional extraction. However, hot water extraction led to extracts with the highest flavonoid content and antioxidant capacity. Scanning electron microscopy analysis showed that all the extraction methods led to cell damage to varying extents.",
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    Optimizing a sustainable ultrasound assisted extraction method for the recovery of polyphenols from lemon by-products : comparison with hot water and organic solvent extractions. / Papoutsis, Konstantinos; Pristijono, Penta; Golding, John B. ; Stathopoulos, Costas E.; Bowyer, Michael C.; Scarlett, Christopher J. ; Vuong, Quan V. .

    In: European Food Research and Technology, Vol. 244, No. 8, 08.2018, p. 1353-1365.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Optimizing a sustainable ultrasound assisted extraction method for the recovery of polyphenols from lemon by-products

    T2 - comparison with hot water and organic solvent extractions

    AU - Papoutsis, Konstantinos

    AU - Pristijono, Penta

    AU - Golding, John B.

    AU - Stathopoulos, Costas E.

    AU - Bowyer, Michael C.

    AU - Scarlett, Christopher J.

    AU - Vuong, Quan V.

    PY - 2018/8

    Y1 - 2018/8

    N2 - Response surface methodology (RSM) based on a three-factor and three-level Box–Behnken design was employed for optimizing the aqueous ultrasound-assisted extraction (AUAE) conditions, including extraction time (35–45 min), extraction temperature (45–55 °C) and ultrasonic power (150–250 W), for the recovery of total phenolic content (TPC) and rutin from lemon by-products. The independent variables and their values were selected on the basis of preliminary experiments, where the effects of five extraction parameters (particle size, extraction time and temperature, ultrasonic power and sample-to-solvent ratio) on TPC and rutin extraction yields were investigated. The yields of TPC and rutin were studied using a second-order polynomial equation. The optimum AUAE conditions for TPC were extraction time of 45 min, extraction temperature of 50 °C and ultrasonic power of 250 W with a predicted value of 18.10 ± 0.24 mg GAE/g dw, while the optimum AUAE conditions for rutin were extraction time of 35 min, extraction temperature of 48 °C and ultrasonic power of 150W with a predicted value of 3.20 ± 0.12 mg/g dw. The extracts obtained at the optimum AUAE conditions were compared with those obtained by a hot water and an organic solvent conventional extraction in terms of TPC, total flavonoid content (TF) and antioxidant capacity. The extracts obtained by AUAE had the same TPC, TF and ferric reducing antioxidant power as those achieved by organic solvent conventional extraction. However, hot water extraction led to extracts with the highest flavonoid content and antioxidant capacity. Scanning electron microscopy analysis showed that all the extraction methods led to cell damage to varying extents.

    AB - Response surface methodology (RSM) based on a three-factor and three-level Box–Behnken design was employed for optimizing the aqueous ultrasound-assisted extraction (AUAE) conditions, including extraction time (35–45 min), extraction temperature (45–55 °C) and ultrasonic power (150–250 W), for the recovery of total phenolic content (TPC) and rutin from lemon by-products. The independent variables and their values were selected on the basis of preliminary experiments, where the effects of five extraction parameters (particle size, extraction time and temperature, ultrasonic power and sample-to-solvent ratio) on TPC and rutin extraction yields were investigated. The yields of TPC and rutin were studied using a second-order polynomial equation. The optimum AUAE conditions for TPC were extraction time of 45 min, extraction temperature of 50 °C and ultrasonic power of 250 W with a predicted value of 18.10 ± 0.24 mg GAE/g dw, while the optimum AUAE conditions for rutin were extraction time of 35 min, extraction temperature of 48 °C and ultrasonic power of 150W with a predicted value of 3.20 ± 0.12 mg/g dw. The extracts obtained at the optimum AUAE conditions were compared with those obtained by a hot water and an organic solvent conventional extraction in terms of TPC, total flavonoid content (TF) and antioxidant capacity. The extracts obtained by AUAE had the same TPC, TF and ferric reducing antioxidant power as those achieved by organic solvent conventional extraction. However, hot water extraction led to extracts with the highest flavonoid content and antioxidant capacity. Scanning electron microscopy analysis showed that all the extraction methods led to cell damage to varying extents.

    U2 - 10.1007/s00217-018-3049-9

    DO - 10.1007/s00217-018-3049-9

    M3 - Article

    VL - 244

    SP - 1353

    EP - 1365

    JO - European Food Research and Technology

    JF - European Food Research and Technology

    SN - 1438-2377

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