Outer membrane protein shifts in biocide-resistant Pseudomonas aeruginosa PAO1

C. L. Winder, I. S. I. Al-Adham, S. M. A. Abdel Malek, T. E. J. Buultjens, A J Horrocks, P. J. Collier

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Benzisothiazolone (BIT), N-methylisothiazolone (MIT) and 5-chloro-N-methylisothiazolone (CMIT) are highly effective biocidal agents and are used as preservatives in a variety of cosmetic preparations. The isothiazolones have proven efficacy against many fungal and bacterial species including Pseudomonas aeruginosa. However, some species are beginning to exhibit resistance towards this group of compounds after extended exposure. This experiment induced resistance in cultures of Ps. aeruginosa exposed to incrementally increasing sub-minimum inhibitory concentrations (MICs) of the isothiazolones in their pure chemical forms. The induced resistance was observed as a gradual increase in MIC with each new passage. The MICs for all three test isothiazolones and a thiol-interactive control compound (thiomersal) increased by approximately twofold during the course of the experiment. The onset of resistance was also observed by reference to the altered presence of an outer membrane protein, designated the T-OMP, in SDS-PAGE preparations. T-OMP was observed to disappear from the biocide-exposed preparations and reappear when the resistance-induced cultures were passaged in the absence of biocide. This reappearance of T-OMP was not accompanied by a complete reversal of induced resistance, but by a small decrease in MIC. The induction of resistance towards one biocide resulted in the development of cross-resistance towards other members of the group and the control, thiomersal. It has been suggested that the disappearance of T-OMP from these preparations is associated with the onset of resistance to the isothiazolones in their Kathon™ form (CMIT and MIT).
    Original languageEnglish
    Pages (from-to)289-295
    Number of pages7
    JournalJournal of Applied Microbiology
    Volume89
    Issue number2
    DOIs
    Publication statusPublished - Aug 2000

    Fingerprint

    Disinfectants
    Microbial Sensitivity Tests
    Pseudomonas aeruginosa
    Membrane Proteins
    Thimerosal
    Sulfhydryl Compounds
    Cosmetics
    Polyacrylamide Gel Electrophoresis
    Control Groups
    2-methyl-4-isothiazolin-3-one

    Cite this

    Winder, C. L., Al-Adham, I. S. I., Abdel Malek, S. M. A., Buultjens, T. E. J., Horrocks, A. J., & Collier, P. J. (2000). Outer membrane protein shifts in biocide-resistant Pseudomonas aeruginosa PAO1. Journal of Applied Microbiology, 89(2), 289-295. https://doi.org/10.1046/j.1365-2672.2000.01119.x
    Winder, C. L. ; Al-Adham, I. S. I. ; Abdel Malek, S. M. A. ; Buultjens, T. E. J. ; Horrocks, A J ; Collier, P. J. / Outer membrane protein shifts in biocide-resistant Pseudomonas aeruginosa PAO1. In: Journal of Applied Microbiology. 2000 ; Vol. 89, No. 2. pp. 289-295.
    @article{d3fbd91def304d269203b5d30fc38f20,
    title = "Outer membrane protein shifts in biocide-resistant Pseudomonas aeruginosa PAO1",
    abstract = "Benzisothiazolone (BIT), N-methylisothiazolone (MIT) and 5-chloro-N-methylisothiazolone (CMIT) are highly effective biocidal agents and are used as preservatives in a variety of cosmetic preparations. The isothiazolones have proven efficacy against many fungal and bacterial species including Pseudomonas aeruginosa. However, some species are beginning to exhibit resistance towards this group of compounds after extended exposure. This experiment induced resistance in cultures of Ps. aeruginosa exposed to incrementally increasing sub-minimum inhibitory concentrations (MICs) of the isothiazolones in their pure chemical forms. The induced resistance was observed as a gradual increase in MIC with each new passage. The MICs for all three test isothiazolones and a thiol-interactive control compound (thiomersal) increased by approximately twofold during the course of the experiment. The onset of resistance was also observed by reference to the altered presence of an outer membrane protein, designated the T-OMP, in SDS-PAGE preparations. T-OMP was observed to disappear from the biocide-exposed preparations and reappear when the resistance-induced cultures were passaged in the absence of biocide. This reappearance of T-OMP was not accompanied by a complete reversal of induced resistance, but by a small decrease in MIC. The induction of resistance towards one biocide resulted in the development of cross-resistance towards other members of the group and the control, thiomersal. It has been suggested that the disappearance of T-OMP from these preparations is associated with the onset of resistance to the isothiazolones in their Kathon™ form (CMIT and MIT).",
    author = "Winder, {C. L.} and Al-Adham, {I. S. I.} and {Abdel Malek}, {S. M. A.} and Buultjens, {T. E. J.} and Horrocks, {A J} and Collier, {P. J.}",
    year = "2000",
    month = "8",
    doi = "10.1046/j.1365-2672.2000.01119.x",
    language = "English",
    volume = "89",
    pages = "289--295",
    journal = "Journal of Applied Microbiology",
    issn = "1364-5072",
    publisher = "Wiley-Blackwell",
    number = "2",

    }

    Winder, CL, Al-Adham, ISI, Abdel Malek, SMA, Buultjens, TEJ, Horrocks, AJ & Collier, PJ 2000, 'Outer membrane protein shifts in biocide-resistant Pseudomonas aeruginosa PAO1', Journal of Applied Microbiology, vol. 89, no. 2, pp. 289-295. https://doi.org/10.1046/j.1365-2672.2000.01119.x

    Outer membrane protein shifts in biocide-resistant Pseudomonas aeruginosa PAO1. / Winder, C. L.; Al-Adham, I. S. I.; Abdel Malek, S. M. A.; Buultjens, T. E. J.; Horrocks, A J; Collier, P. J.

    In: Journal of Applied Microbiology, Vol. 89, No. 2, 08.2000, p. 289-295.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Outer membrane protein shifts in biocide-resistant Pseudomonas aeruginosa PAO1

    AU - Winder, C. L.

    AU - Al-Adham, I. S. I.

    AU - Abdel Malek, S. M. A.

    AU - Buultjens, T. E. J.

    AU - Horrocks, A J

    AU - Collier, P. J.

    PY - 2000/8

    Y1 - 2000/8

    N2 - Benzisothiazolone (BIT), N-methylisothiazolone (MIT) and 5-chloro-N-methylisothiazolone (CMIT) are highly effective biocidal agents and are used as preservatives in a variety of cosmetic preparations. The isothiazolones have proven efficacy against many fungal and bacterial species including Pseudomonas aeruginosa. However, some species are beginning to exhibit resistance towards this group of compounds after extended exposure. This experiment induced resistance in cultures of Ps. aeruginosa exposed to incrementally increasing sub-minimum inhibitory concentrations (MICs) of the isothiazolones in their pure chemical forms. The induced resistance was observed as a gradual increase in MIC with each new passage. The MICs for all three test isothiazolones and a thiol-interactive control compound (thiomersal) increased by approximately twofold during the course of the experiment. The onset of resistance was also observed by reference to the altered presence of an outer membrane protein, designated the T-OMP, in SDS-PAGE preparations. T-OMP was observed to disappear from the biocide-exposed preparations and reappear when the resistance-induced cultures were passaged in the absence of biocide. This reappearance of T-OMP was not accompanied by a complete reversal of induced resistance, but by a small decrease in MIC. The induction of resistance towards one biocide resulted in the development of cross-resistance towards other members of the group and the control, thiomersal. It has been suggested that the disappearance of T-OMP from these preparations is associated with the onset of resistance to the isothiazolones in their Kathon™ form (CMIT and MIT).

    AB - Benzisothiazolone (BIT), N-methylisothiazolone (MIT) and 5-chloro-N-methylisothiazolone (CMIT) are highly effective biocidal agents and are used as preservatives in a variety of cosmetic preparations. The isothiazolones have proven efficacy against many fungal and bacterial species including Pseudomonas aeruginosa. However, some species are beginning to exhibit resistance towards this group of compounds after extended exposure. This experiment induced resistance in cultures of Ps. aeruginosa exposed to incrementally increasing sub-minimum inhibitory concentrations (MICs) of the isothiazolones in their pure chemical forms. The induced resistance was observed as a gradual increase in MIC with each new passage. The MICs for all three test isothiazolones and a thiol-interactive control compound (thiomersal) increased by approximately twofold during the course of the experiment. The onset of resistance was also observed by reference to the altered presence of an outer membrane protein, designated the T-OMP, in SDS-PAGE preparations. T-OMP was observed to disappear from the biocide-exposed preparations and reappear when the resistance-induced cultures were passaged in the absence of biocide. This reappearance of T-OMP was not accompanied by a complete reversal of induced resistance, but by a small decrease in MIC. The induction of resistance towards one biocide resulted in the development of cross-resistance towards other members of the group and the control, thiomersal. It has been suggested that the disappearance of T-OMP from these preparations is associated with the onset of resistance to the isothiazolones in their Kathon™ form (CMIT and MIT).

    U2 - 10.1046/j.1365-2672.2000.01119.x

    DO - 10.1046/j.1365-2672.2000.01119.x

    M3 - Article

    VL - 89

    SP - 289

    EP - 295

    JO - Journal of Applied Microbiology

    JF - Journal of Applied Microbiology

    SN - 1364-5072

    IS - 2

    ER -