Oxygenating blubber: a challenge for fat animals

Research output: Contribution to conferenceAbstract

Abstract

Adipose tissue expansion can cause reduced blood flow and tissue hypoxia. However, seals require a thick blubber layer for survival. How blubber maintains adequate oxygenation is unknown. Here, we examine blubber depth and fatness effects on tissue oxygenation. Body condition was assessed in juvenile grey seals using morphometric data, blubber depth and photogrammetry. Systemic blood oxygen saturation (SpO2 ), heart rate and breathing rate were monitored throughout sampling. Blubber oxyhaemoglobin and deoxygenated haemoglobin were measured in dorsal flank blubber under appropriate sedation and anaesthesia using a novel non-invasive technique: Near Infrared Spectroscopy (NIRS). Simultaneously, temperature and partial pressure of oxygen (pO2) was measured at different blubber depths, with an optical, nonconsuming oxygen probe. Total blubber depth ranged between 10 mm and 28 mm (mean = 18.1 mm). Despite constant systemic blood saturation throughout sampling and among individuals (SpO2 = 89.4% ± 6.6), there were large inter-individual pO2 differences, ranging between 11 mmHg and 59 mmHg (mean = 32 mmHg). pO2 inter-site variability and variation over time were tested with no significant effect (n = 4, p > 0.05). Linear mixed effects analysis (LMM) showed that pO2 varies irrespective of the depth of the measurement, but showed a negative relationship with body fatness (n = 10, p < 0.05, R2 marginal = 0.31, R2 conditional = 0.97), consistent with findings in mice. NIRS data analysis is ongoing to investigate oxygen supply to blubber in relation to pO2. Further histological and molecular analysis are also underway to explore vascularity and consequences of hypoxia for blubber.
Original languageEnglish
Publication statusPublished - 3 Jul 2019
EventSociety of Experimental Biology Annual Conference - FIBES II Conference and Exhibition Centre, Seville, Spain
Duration: 2 Jul 20195 Jul 2019
https://www.sebiology.org/events/event/seb-seville-2019

Conference

ConferenceSociety of Experimental Biology Annual Conference
CountrySpain
CitySeville
Period2/07/195/07/19
Internet address

Fingerprint Dive into the research topics of 'Oxygenating blubber: a challenge for fat animals'. Together they form a unique fingerprint.

  • Cite this