Oxygenating blubber: a challenge for fat animals

Research output: Contribution to conferenceAbstract

Abstract

Adipose tissue expansion can cause reduced blood flow and tissue hypoxia. However, seals require a thick blubber layer for survival. How blubber maintains adequate oxygenation is unknown. Here, we examine blubber depth and fatness effects on tissue oxygenation. Body condition was assessed in juvenile grey seals using morphometric data, blubber depth and photogrammetry. Systemic blood oxygen saturation (SpO2 ), heart rate and breathing rate were monitored throughout sampling. Blubber oxyhaemoglobin and deoxygenated haemoglobin were measured in dorsal flank blubber under appropriate sedation and anaesthesia using a novel non-invasive technique: Near Infrared Spectroscopy (NIRS). Simultaneously, temperature and partial pressure of oxygen (pO2) was measured at different blubber depths, with an optical, nonconsuming oxygen probe. Total blubber depth ranged between 10 mm and 28 mm (mean = 18.1 mm). Despite constant systemic blood saturation throughout sampling and among individuals (SpO2 = 89.4% ± 6.6), there were large inter-individual pO2 differences, ranging between 11 mmHg and 59 mmHg (mean = 32 mmHg). pO2 inter-site variability and variation over time were tested with no significant effect (n = 4, p > 0.05). Linear mixed effects analysis (LMM) showed that pO2 varies irrespective of the depth of the measurement, but showed a negative relationship with body fatness (n = 10, p < 0.05, R2 marginal = 0.31, R2 conditional = 0.97), consistent with findings in mice. NIRS data analysis is ongoing to investigate oxygen supply to blubber in relation to pO2. Further histological and molecular analysis are also underway to explore vascularity and consequences of hypoxia for blubber.
Original languageEnglish
Publication statusPublished - Jul 2019
EventSociety of Experimental Biology Annual Conference - FIBES II Conference and Exhibition Centre, Seville, Spain
Duration: 2 Jul 20195 Jul 2019
https://www.sebiology.org/events/event/seb-seville-2019

Conference

ConferenceSociety of Experimental Biology Annual Conference
CountrySpain
CitySeville
Period2/07/195/07/19
Internet address

Fingerprint

Fats
Oxygen
Near-Infrared Spectroscopy
Photogrammetry
Earless Seals
Tissue Expansion
Oxyhemoglobins
Partial Pressure
Individuality
Adipose Tissue
Respiration
Hemoglobins
Anesthesia
Heart Rate
Temperature
Hypoxia

Cite this

Oller Lopez, L., Rocha, J., McKnight, C., & Bennett, K. A. (2019). Oxygenating blubber: a challenge for fat animals. Abstract from Society of Experimental Biology Annual Conference, Seville, Spain.
Oller Lopez, Laura ; Rocha, Joel ; McKnight, Chris ; Bennett, Kimberley A. . / Oxygenating blubber : a challenge for fat animals. Abstract from Society of Experimental Biology Annual Conference, Seville, Spain.
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title = "Oxygenating blubber: a challenge for fat animals",
abstract = "Adipose tissue expansion can cause reduced blood flow and tissue hypoxia. However, seals require a thick blubber layer for survival. How blubber maintains adequate oxygenation is unknown. Here, we examine blubber depth and fatness effects on tissue oxygenation. Body condition was assessed in juvenile grey seals using morphometric data, blubber depth and photogrammetry. Systemic blood oxygen saturation (SpO2 ), heart rate and breathing rate were monitored throughout sampling. Blubber oxyhaemoglobin and deoxygenated haemoglobin were measured in dorsal flank blubber under appropriate sedation and anaesthesia using a novel non-invasive technique: Near Infrared Spectroscopy (NIRS). Simultaneously, temperature and partial pressure of oxygen (pO2) was measured at different blubber depths, with an optical, nonconsuming oxygen probe. Total blubber depth ranged between 10 mm and 28 mm (mean = 18.1 mm). Despite constant systemic blood saturation throughout sampling and among individuals (SpO2 = 89.4{\%} ± 6.6), there were large inter-individual pO2 differences, ranging between 11 mmHg and 59 mmHg (mean = 32 mmHg). pO2 inter-site variability and variation over time were tested with no significant effect (n = 4, p > 0.05). Linear mixed effects analysis (LMM) showed that pO2 varies irrespective of the depth of the measurement, but showed a negative relationship with body fatness (n = 10, p < 0.05, R2 marginal = 0.31, R2 conditional = 0.97), consistent with findings in mice. NIRS data analysis is ongoing to investigate oxygen supply to blubber in relation to pO2. Further histological and molecular analysis are also underway to explore vascularity and consequences of hypoxia for blubber.",
author = "{Oller Lopez}, Laura and Joel Rocha and Chris McKnight and Bennett, {Kimberley A.}",
year = "2019",
month = "7",
language = "English",
note = "Society of Experimental Biology Annual Conference ; Conference date: 02-07-2019 Through 05-07-2019",
url = "https://www.sebiology.org/events/event/seb-seville-2019",

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Oller Lopez, L, Rocha, J, McKnight, C & Bennett, KA 2019, 'Oxygenating blubber: a challenge for fat animals' Society of Experimental Biology Annual Conference, Seville, Spain, 2/07/19 - 5/07/19, .

Oxygenating blubber : a challenge for fat animals. / Oller Lopez, Laura; Rocha, Joel; McKnight, Chris; Bennett, Kimberley A. .

2019. Abstract from Society of Experimental Biology Annual Conference, Seville, Spain.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Oxygenating blubber

T2 - a challenge for fat animals

AU - Oller Lopez, Laura

AU - Rocha, Joel

AU - McKnight, Chris

AU - Bennett, Kimberley A.

PY - 2019/7

Y1 - 2019/7

N2 - Adipose tissue expansion can cause reduced blood flow and tissue hypoxia. However, seals require a thick blubber layer for survival. How blubber maintains adequate oxygenation is unknown. Here, we examine blubber depth and fatness effects on tissue oxygenation. Body condition was assessed in juvenile grey seals using morphometric data, blubber depth and photogrammetry. Systemic blood oxygen saturation (SpO2 ), heart rate and breathing rate were monitored throughout sampling. Blubber oxyhaemoglobin and deoxygenated haemoglobin were measured in dorsal flank blubber under appropriate sedation and anaesthesia using a novel non-invasive technique: Near Infrared Spectroscopy (NIRS). Simultaneously, temperature and partial pressure of oxygen (pO2) was measured at different blubber depths, with an optical, nonconsuming oxygen probe. Total blubber depth ranged between 10 mm and 28 mm (mean = 18.1 mm). Despite constant systemic blood saturation throughout sampling and among individuals (SpO2 = 89.4% ± 6.6), there were large inter-individual pO2 differences, ranging between 11 mmHg and 59 mmHg (mean = 32 mmHg). pO2 inter-site variability and variation over time were tested with no significant effect (n = 4, p > 0.05). Linear mixed effects analysis (LMM) showed that pO2 varies irrespective of the depth of the measurement, but showed a negative relationship with body fatness (n = 10, p < 0.05, R2 marginal = 0.31, R2 conditional = 0.97), consistent with findings in mice. NIRS data analysis is ongoing to investigate oxygen supply to blubber in relation to pO2. Further histological and molecular analysis are also underway to explore vascularity and consequences of hypoxia for blubber.

AB - Adipose tissue expansion can cause reduced blood flow and tissue hypoxia. However, seals require a thick blubber layer for survival. How blubber maintains adequate oxygenation is unknown. Here, we examine blubber depth and fatness effects on tissue oxygenation. Body condition was assessed in juvenile grey seals using morphometric data, blubber depth and photogrammetry. Systemic blood oxygen saturation (SpO2 ), heart rate and breathing rate were monitored throughout sampling. Blubber oxyhaemoglobin and deoxygenated haemoglobin were measured in dorsal flank blubber under appropriate sedation and anaesthesia using a novel non-invasive technique: Near Infrared Spectroscopy (NIRS). Simultaneously, temperature and partial pressure of oxygen (pO2) was measured at different blubber depths, with an optical, nonconsuming oxygen probe. Total blubber depth ranged between 10 mm and 28 mm (mean = 18.1 mm). Despite constant systemic blood saturation throughout sampling and among individuals (SpO2 = 89.4% ± 6.6), there were large inter-individual pO2 differences, ranging between 11 mmHg and 59 mmHg (mean = 32 mmHg). pO2 inter-site variability and variation over time were tested with no significant effect (n = 4, p > 0.05). Linear mixed effects analysis (LMM) showed that pO2 varies irrespective of the depth of the measurement, but showed a negative relationship with body fatness (n = 10, p < 0.05, R2 marginal = 0.31, R2 conditional = 0.97), consistent with findings in mice. NIRS data analysis is ongoing to investigate oxygen supply to blubber in relation to pO2. Further histological and molecular analysis are also underway to explore vascularity and consequences of hypoxia for blubber.

M3 - Abstract

ER -

Oller Lopez L, Rocha J, McKnight C, Bennett KA. Oxygenating blubber: a challenge for fat animals. 2019. Abstract from Society of Experimental Biology Annual Conference, Seville, Spain.