Perturbation of enzyme activity in cells of the fission yeast Schizosaccharomyces pombe subjected to continuous-flow centrifugation

Graeme M. Walker, James C. Thomson, J. Colin Slaughter, John H. Duffus

Research output: Contribution to journalArticle

Abstract

Fluctuations in glutamine synthetase activity were observed when exponentially dividing cells of Schizosaccharomyces pombe were synchronized by continuous-flow centrifugation. These fluctuations were not seen in a control culture in which cells were allowed to grow asynchronously following filtration. However, when an asynchronous control culture was prepared by allowing cells to pass quickly through the continuous-action rotor without size selection, marked fluctuations in glutamine synthetase activity occurred. It is concluded that when the continuous-flow size selection method of cell synchronization is used, suitable control experiments are essential before any conclusions can be drawn about how enzyme synthesis is regulated during the cell cycle.
Original languageEnglish
Pages (from-to)543-546
Number of pages4
JournalJournal of General Microbiology
Volume119
Issue number2
DOIs
StatePublished - 1 Aug 1980

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Schizosaccharomyces
Centrifugation
Enzymes
Glutamate-Ammonia Ligase
Cell Cycle

Cite this

Walker, Graeme M.; Thomson, James C.; Slaughter, J. Colin; Duffus, John H. / Perturbation of enzyme activity in cells of the fission yeast Schizosaccharomyces pombe subjected to continuous-flow centrifugation.

In: Journal of General Microbiology, Vol. 119, No. 2, 01.08.1980, p. 543-546.

Research output: Contribution to journalArticle

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Perturbation of enzyme activity in cells of the fission yeast Schizosaccharomyces pombe subjected to continuous-flow centrifugation. / Walker, Graeme M.; Thomson, James C.; Slaughter, J. Colin; Duffus, John H.

In: Journal of General Microbiology, Vol. 119, No. 2, 01.08.1980, p. 543-546.

Research output: Contribution to journalArticle

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