Abstract
Fluctuations in glutamine synthetase activity were observed when exponentially dividing cells of Schizosaccharomyces pombe were synchronized by continuous-flow centrifugation. These fluctuations were not seen in a control culture in which cells were allowed to grow asynchronously following filtration. However, when an asynchronous control culture was prepared by allowing cells to pass quickly through the continuous-action rotor without size selection, marked fluctuations in glutamine synthetase activity occurred. It is concluded that when the continuous-flow size selection method of cell synchronization is used, suitable control experiments are essential before any conclusions can be drawn about how enzyme synthesis is regulated during the cell cycle.
| Original language | English |
|---|---|
| Pages (from-to) | 543-546 |
| Number of pages | 4 |
| Journal | Journal of General Microbiology |
| Volume | 119 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Aug 1980 |
Keywords
- Schizosaccharomyces