PGC-1α transcriptional response and mitochondrial adaptation to acute exercise is maintained in skeletal muscle of sedentary elderly males

James N. Cobley, J. D. Bartlett, A. Kayani, S. W. Murray, J. Louhelainen, T. Donovan, S. Waldron, Warren Gregson, Jatin G. Burniston, James P. Morton, Graeme L. Close

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The aim of the present study was to examine the effects of ageing and training status on (1) markers of skeletal muscle mitochondrial content and (2) the ability to activate the acute signalling pathways associated with regulating exercise-induced mitochondrial biogenesis. Muscle biopsies were obtained from the vastus lateralis muscle of young untrained (24 ± 4 years, n = 6; YU), young trained (22 ± 3 years, n = 6; YT), old untrained (65 ± 6 years, n = 6; OU) and old trained (64 ± 3 years, n = 6; OT) healthy males before and after (3 h and 3 days post-exercise) completion of high-intensity interval cycling exercise. In resting muscle, lifelong training preserved mtDNA, PGC-1α and COXIV protein content such that muscles from OT individuals were comparable to muscles from both YU and YT individuals, whereas lifelong sedentary behaviour reduced such markers of mitochondrial content. Regardless of age or training status, acute exercise induced comparable increases in p38MAPK phosphorylation immediately post-exercise, PGC-1α and COXIV mRNA expression at 3 h post-exercise and COXIV protein at 3 days post-exercise. Data demonstrate that lifelong endurance training preserves skeletal muscle PGC-1α content and that despite the mitochondrial dysfunction typically observed with sedentary ageing, muscles from sedentary elderly individuals retain the capacity to activate the acute signalling pathways associated with regulating the early processes of mitochondrial biogenesis. We consider our data to have immediate translational potential as they highlight the potential therapeutic effects of exercise to induce skeletal muscle mitochondrial biogenesis persist late in adulthood, even after a lifetime of physical inactivity.
Original languageEnglish
Pages (from-to)621–631
Number of pages11
JournalBiogerontology
Volume13
Issue number6
DOIs
Publication statusPublished - Dec 2012

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Skeletal Muscle
Exercise
Muscles
Organelle Biogenesis
Quadriceps Muscle
Therapeutic Uses
Mitochondrial DNA
Proteins
Phosphorylation
Biopsy
Messenger RNA

Cite this

Cobley, J. N., Bartlett, J. D., Kayani, A., Murray, S. W., Louhelainen, J., Donovan, T., ... Close, G. L. (2012). PGC-1α transcriptional response and mitochondrial adaptation to acute exercise is maintained in skeletal muscle of sedentary elderly males. Biogerontology, 13(6), 621–631. https://doi.org/10.1007/s10522-012-9408-1
Cobley, James N. ; Bartlett, J. D. ; Kayani, A. ; Murray, S. W. ; Louhelainen, J. ; Donovan, T. ; Waldron, S. ; Gregson, Warren ; Burniston, Jatin G. ; Morton, James P. ; Close, Graeme L. / PGC-1α transcriptional response and mitochondrial adaptation to acute exercise is maintained in skeletal muscle of sedentary elderly males. In: Biogerontology. 2012 ; Vol. 13, No. 6. pp. 621–631.
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Cobley, JN, Bartlett, JD, Kayani, A, Murray, SW, Louhelainen, J, Donovan, T, Waldron, S, Gregson, W, Burniston, JG, Morton, JP & Close, GL 2012, 'PGC-1α transcriptional response and mitochondrial adaptation to acute exercise is maintained in skeletal muscle of sedentary elderly males', Biogerontology, vol. 13, no. 6, pp. 621–631. https://doi.org/10.1007/s10522-012-9408-1

PGC-1α transcriptional response and mitochondrial adaptation to acute exercise is maintained in skeletal muscle of sedentary elderly males. / Cobley, James N.; Bartlett, J. D.; Kayani, A.; Murray, S. W.; Louhelainen, J.; Donovan, T.; Waldron, S.; Gregson, Warren; Burniston, Jatin G.; Morton, James P.; Close, Graeme L.

In: Biogerontology, Vol. 13, No. 6, 12.2012, p. 621–631.

Research output: Contribution to journalArticle

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T1 - PGC-1α transcriptional response and mitochondrial adaptation to acute exercise is maintained in skeletal muscle of sedentary elderly males

AU - Cobley, James N.

AU - Bartlett, J. D.

AU - Kayani, A.

AU - Murray, S. W.

AU - Louhelainen, J.

AU - Donovan, T.

AU - Waldron, S.

AU - Gregson, Warren

AU - Burniston, Jatin G.

AU - Morton, James P.

AU - Close, Graeme L.

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N2 - The aim of the present study was to examine the effects of ageing and training status on (1) markers of skeletal muscle mitochondrial content and (2) the ability to activate the acute signalling pathways associated with regulating exercise-induced mitochondrial biogenesis. Muscle biopsies were obtained from the vastus lateralis muscle of young untrained (24 ± 4 years, n = 6; YU), young trained (22 ± 3 years, n = 6; YT), old untrained (65 ± 6 years, n = 6; OU) and old trained (64 ± 3 years, n = 6; OT) healthy males before and after (3 h and 3 days post-exercise) completion of high-intensity interval cycling exercise. In resting muscle, lifelong training preserved mtDNA, PGC-1α and COXIV protein content such that muscles from OT individuals were comparable to muscles from both YU and YT individuals, whereas lifelong sedentary behaviour reduced such markers of mitochondrial content. Regardless of age or training status, acute exercise induced comparable increases in p38MAPK phosphorylation immediately post-exercise, PGC-1α and COXIV mRNA expression at 3 h post-exercise and COXIV protein at 3 days post-exercise. Data demonstrate that lifelong endurance training preserves skeletal muscle PGC-1α content and that despite the mitochondrial dysfunction typically observed with sedentary ageing, muscles from sedentary elderly individuals retain the capacity to activate the acute signalling pathways associated with regulating the early processes of mitochondrial biogenesis. We consider our data to have immediate translational potential as they highlight the potential therapeutic effects of exercise to induce skeletal muscle mitochondrial biogenesis persist late in adulthood, even after a lifetime of physical inactivity.

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DO - 10.1007/s10522-012-9408-1

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