Physical and chemical processes and the morphofunctional characteristics of human erythrocytes in hyperglycaemia

Victor V. Revin*, Natalia A. Klenova, Natalia V. Gromova, Igor P. Grunyushkin, Ilia N. Solomadin, Alexander Y. Tychkov, Anastasia A. Pestryakova, Anna V. Sadykhova, Elvira S. Revina, Ksenia V. Prosnikova, Jean Christophe Bourdon, Nikolai Zhelev

*Corresponding author for this work

    Research output: Contribution to journalArticle

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    Abstract

    Background: This study examines the effect of graduated hyperglycaemia on the state and oxygen-binding ability of hemoglobin, the correlation of phospholipid fractions and their metabolites in the membrane, the activity of proteolytic enzymes and the morphofunctional state of erythrocytes. Methods: Conformational changes in the molecule of hemoglobin were determined by Raman spectroscopy. The structure of the erythrocytes was analyzed using laser interference microscopy (LIM). To determine the activity of NADN-methemoglobinreductase, we used the P.G. Board method. The degree of glycosylation of the erythrocyte membranes was determined using a method previously described by Felkoren et al. Lipid extraction was performed using the Bligh and Dyer method. Detection of the phospholipids was performed using V. E. Vaskovsky method. Results: Conditions of hyperglycaemia are characterized by a low affinity of hemoglobin to oxygen, which is manifested as a parallel decrease in the content of hemoglobin oxyform and the growth of deoxyform, methemoglobin and membrane-bound hemoglobin. The degree of glycosylation of membrane proteins and hemoglobin is high. For example, in the case of hyperglycaemia, erythrocytic membranes reduce the content of all phospholipid fractions with a simultaneous increase in lysoforms, free fatty acids and the diacylglycerol (DAG). Step wise hyperglycaemia in incubation medium and human erythrocytes results in an increased content of peptide components and general trypsin-like activity in the cytosol, with a simultaneous decreased activity of µ-calpain and caspase 3. Conclusions: Metabolic disorders and damage of cell membranes during hyperglycaemia cause an increase in the population of echinocytes and spherocytes. The resulting disorders are accompanied with a high probability of intravascular haemolysis.

    Original languageEnglish
    Article number606
    Number of pages8
    JournalFrontiers in Physiology
    Volume8
    Early online date30 Aug 2017
    DOIs
    Publication statusPublished - 30 Aug 2017

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    Chemical Phenomena
    Physical Phenomena
    Hyperglycemia
    Hemoglobins
    Erythrocytes
    Phospholipids
    Glycosylation
    Membranes
    Spherocytes
    Interference Microscopy
    Oxygen
    Methemoglobin
    Calpain
    Raman Spectrum Analysis
    Diglycerides
    Erythrocyte Membrane
    Hemolysis
    Nonesterified Fatty Acids
    Confocal Microscopy
    Caspase 3

    Cite this

    Revin, V. V., Klenova, N. A., Gromova, N. V., Grunyushkin, I. P., Solomadin, I. N., Tychkov, A. Y., ... Zhelev, N. (2017). Physical and chemical processes and the morphofunctional characteristics of human erythrocytes in hyperglycaemia. Frontiers in Physiology, 8, [606]. https://doi.org/10.3389/fphys.2017.00606
    Revin, Victor V. ; Klenova, Natalia A. ; Gromova, Natalia V. ; Grunyushkin, Igor P. ; Solomadin, Ilia N. ; Tychkov, Alexander Y. ; Pestryakova, Anastasia A. ; Sadykhova, Anna V. ; Revina, Elvira S. ; Prosnikova, Ksenia V. ; Bourdon, Jean Christophe ; Zhelev, Nikolai. / Physical and chemical processes and the morphofunctional characteristics of human erythrocytes in hyperglycaemia. In: Frontiers in Physiology. 2017 ; Vol. 8.
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    title = "Physical and chemical processes and the morphofunctional characteristics of human erythrocytes in hyperglycaemia",
    abstract = "Background: This study examines the effect of graduated hyperglycaemia on the state and oxygen-binding ability of hemoglobin, the correlation of phospholipid fractions and their metabolites in the membrane, the activity of proteolytic enzymes and the morphofunctional state of erythrocytes. Methods: Conformational changes in the molecule of hemoglobin were determined by Raman spectroscopy. The structure of the erythrocytes was analyzed using laser interference microscopy (LIM). To determine the activity of NADN-methemoglobinreductase, we used the P.G. Board method. The degree of glycosylation of the erythrocyte membranes was determined using a method previously described by Felkoren et al. Lipid extraction was performed using the Bligh and Dyer method. Detection of the phospholipids was performed using V. E. Vaskovsky method. Results: Conditions of hyperglycaemia are characterized by a low affinity of hemoglobin to oxygen, which is manifested as a parallel decrease in the content of hemoglobin oxyform and the growth of deoxyform, methemoglobin and membrane-bound hemoglobin. The degree of glycosylation of membrane proteins and hemoglobin is high. For example, in the case of hyperglycaemia, erythrocytic membranes reduce the content of all phospholipid fractions with a simultaneous increase in lysoforms, free fatty acids and the diacylglycerol (DAG). Step wise hyperglycaemia in incubation medium and human erythrocytes results in an increased content of peptide components and general trypsin-like activity in the cytosol, with a simultaneous decreased activity of µ-calpain and caspase 3. Conclusions: Metabolic disorders and damage of cell membranes during hyperglycaemia cause an increase in the population of echinocytes and spherocytes. The resulting disorders are accompanied with a high probability of intravascular haemolysis.",
    author = "Revin, {Victor V.} and Klenova, {Natalia A.} and Gromova, {Natalia V.} and Grunyushkin, {Igor P.} and Solomadin, {Ilia N.} and Tychkov, {Alexander Y.} and Pestryakova, {Anastasia A.} and Sadykhova, {Anna V.} and Revina, {Elvira S.} and Prosnikova, {Ksenia V.} and Bourdon, {Jean Christophe} and Nikolai Zhelev",
    year = "2017",
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    Revin, VV, Klenova, NA, Gromova, NV, Grunyushkin, IP, Solomadin, IN, Tychkov, AY, Pestryakova, AA, Sadykhova, AV, Revina, ES, Prosnikova, KV, Bourdon, JC & Zhelev, N 2017, 'Physical and chemical processes and the morphofunctional characteristics of human erythrocytes in hyperglycaemia', Frontiers in Physiology, vol. 8, 606. https://doi.org/10.3389/fphys.2017.00606

    Physical and chemical processes and the morphofunctional characteristics of human erythrocytes in hyperglycaemia. / Revin, Victor V.; Klenova, Natalia A.; Gromova, Natalia V.; Grunyushkin, Igor P.; Solomadin, Ilia N.; Tychkov, Alexander Y.; Pestryakova, Anastasia A.; Sadykhova, Anna V.; Revina, Elvira S.; Prosnikova, Ksenia V.; Bourdon, Jean Christophe; Zhelev, Nikolai.

    In: Frontiers in Physiology, Vol. 8, 606, 30.08.2017.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Physical and chemical processes and the morphofunctional characteristics of human erythrocytes in hyperglycaemia

    AU - Revin, Victor V.

    AU - Klenova, Natalia A.

    AU - Gromova, Natalia V.

    AU - Grunyushkin, Igor P.

    AU - Solomadin, Ilia N.

    AU - Tychkov, Alexander Y.

    AU - Pestryakova, Anastasia A.

    AU - Sadykhova, Anna V.

    AU - Revina, Elvira S.

    AU - Prosnikova, Ksenia V.

    AU - Bourdon, Jean Christophe

    AU - Zhelev, Nikolai

    PY - 2017/8/30

    Y1 - 2017/8/30

    N2 - Background: This study examines the effect of graduated hyperglycaemia on the state and oxygen-binding ability of hemoglobin, the correlation of phospholipid fractions and their metabolites in the membrane, the activity of proteolytic enzymes and the morphofunctional state of erythrocytes. Methods: Conformational changes in the molecule of hemoglobin were determined by Raman spectroscopy. The structure of the erythrocytes was analyzed using laser interference microscopy (LIM). To determine the activity of NADN-methemoglobinreductase, we used the P.G. Board method. The degree of glycosylation of the erythrocyte membranes was determined using a method previously described by Felkoren et al. Lipid extraction was performed using the Bligh and Dyer method. Detection of the phospholipids was performed using V. E. Vaskovsky method. Results: Conditions of hyperglycaemia are characterized by a low affinity of hemoglobin to oxygen, which is manifested as a parallel decrease in the content of hemoglobin oxyform and the growth of deoxyform, methemoglobin and membrane-bound hemoglobin. The degree of glycosylation of membrane proteins and hemoglobin is high. For example, in the case of hyperglycaemia, erythrocytic membranes reduce the content of all phospholipid fractions with a simultaneous increase in lysoforms, free fatty acids and the diacylglycerol (DAG). Step wise hyperglycaemia in incubation medium and human erythrocytes results in an increased content of peptide components and general trypsin-like activity in the cytosol, with a simultaneous decreased activity of µ-calpain and caspase 3. Conclusions: Metabolic disorders and damage of cell membranes during hyperglycaemia cause an increase in the population of echinocytes and spherocytes. The resulting disorders are accompanied with a high probability of intravascular haemolysis.

    AB - Background: This study examines the effect of graduated hyperglycaemia on the state and oxygen-binding ability of hemoglobin, the correlation of phospholipid fractions and their metabolites in the membrane, the activity of proteolytic enzymes and the morphofunctional state of erythrocytes. Methods: Conformational changes in the molecule of hemoglobin were determined by Raman spectroscopy. The structure of the erythrocytes was analyzed using laser interference microscopy (LIM). To determine the activity of NADN-methemoglobinreductase, we used the P.G. Board method. The degree of glycosylation of the erythrocyte membranes was determined using a method previously described by Felkoren et al. Lipid extraction was performed using the Bligh and Dyer method. Detection of the phospholipids was performed using V. E. Vaskovsky method. Results: Conditions of hyperglycaemia are characterized by a low affinity of hemoglobin to oxygen, which is manifested as a parallel decrease in the content of hemoglobin oxyform and the growth of deoxyform, methemoglobin and membrane-bound hemoglobin. The degree of glycosylation of membrane proteins and hemoglobin is high. For example, in the case of hyperglycaemia, erythrocytic membranes reduce the content of all phospholipid fractions with a simultaneous increase in lysoforms, free fatty acids and the diacylglycerol (DAG). Step wise hyperglycaemia in incubation medium and human erythrocytes results in an increased content of peptide components and general trypsin-like activity in the cytosol, with a simultaneous decreased activity of µ-calpain and caspase 3. Conclusions: Metabolic disorders and damage of cell membranes during hyperglycaemia cause an increase in the population of echinocytes and spherocytes. The resulting disorders are accompanied with a high probability of intravascular haemolysis.

    U2 - 10.3389/fphys.2017.00606

    DO - 10.3389/fphys.2017.00606

    M3 - Article

    AN - SCOPUS:85028472875

    VL - 8

    JO - Frontiers in Physiology

    JF - Frontiers in Physiology

    SN - 1664-042X

    M1 - 606

    ER -