Physiological responses of Crabtree positive and Crabtree negative yeasts to glucose upshifts in a chemostat

F. R. Wardrop, G. Liti, G. Cardinali, G. M. Walker*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    Growth and metabolic differences between a Crabtree positive yeast, Saccharomyces cerevisiae, and a Crabtree negative yeast, Kluyveromyces marxianus, were investigated using chemostat cultures under glucose limitation. When subjected to glucose upshifts ("pulses" or sudden increases in glucose availability), several physiological differences between these two yeasts became apparent. Whilst the production Of ethanol was very rapid in S. cerevisiae, there was a complete lack of alcoholic fermentation in K. marxianus. Glucose utilisation kinetics also differed, with S. cerevisiae rapidly consuming 50% of the additional available glucose (through fermentation) whilst K. marxianus was only utilised 10% of the glucose (through respiration). These and other differences suggest possible evolutionary advantages of the Crabtree effect in the metabolism of sugar rich environments by certain yeasts.
    Original languageEnglish
    Pages (from-to)103-114
    Number of pages11
    JournalAnnals of Microbiology
    Volume54
    Issue number1
    Publication statusPublished - 2004

    Keywords

    • Crabtree effect
    • Saccharomyces cerevisiae
    • Respiration
    • Fermentation
    • Kluyveromyces marxianus

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