Plasmid carriage can limit bacteria–phage coevolution

Ellie Harrison, Julie Truman, Rosanna Wright, Andrew J. Spiers, Steve Paterson, Michael A. Brockhurst

    Research output: Contribution to journalArticle

    7 Citations (Scopus)
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    Abstract

    Coevolution with bacteriophages is a major selective force shaping bacterial populations and communities. A variety of both environmental and genetic factors has been shown to influence the mode and tempo of bacteria–phage coevolution. Here, we test the effects that carriage of a large conjugative plasmid, pQBR103, had on antagonistic coevolution between the bacterium Pseudomonas fluorescens and its phage, SBW25ϕ2. Plasmid carriage limited bacteria–phage coevolution; bacteria evolved lower phage-resistance and phages evolved lower infectivity in plasmid-carrying compared with plasmid-free populations. These differences were not explained by effects of plasmid carriage on the costs of phage resistance mutations. Surprisingly, in the presence of phages, plasmid carriage resulted in the evolution of high frequencies of mucoid bacterial colonies. Mucoidy can provide weak partial resistance against SBW25ϕ2, which may have limited selection for qualitative resistance mutations in our experiments. Taken together, our results suggest that plasmids can have evolutionary consequences for bacteria that go beyond the direct phenotypic effects of their accessory gene cargo.
    Original languageEnglish
    Article number20150361
    Number of pages4
    JournalBiology Letters
    Volume11
    Issue number8
    DOIs
    Publication statusPublished - 12 Aug 2015

    Fingerprint

    coevolution
    plasmids
    bacteriophages
    Bacteriophages
    Plasmids
    Bacteria
    bacteria
    mutation
    Pseudomonas fluorescens
    Mutation
    Population
    pathogenicity
    Costs and Cost Analysis
    Genes
    genes
    testing

    Cite this

    Harrison, E., Truman, J., Wright, R., Spiers, A. J., Paterson, S., & Brockhurst, M. A. (2015). Plasmid carriage can limit bacteria–phage coevolution. Biology Letters, 11(8), [20150361]. https://doi.org/10.1098/rsbl.2015.0361
    Harrison, Ellie ; Truman, Julie ; Wright, Rosanna ; Spiers, Andrew J. ; Paterson, Steve ; Brockhurst, Michael A. / Plasmid carriage can limit bacteria–phage coevolution. In: Biology Letters. 2015 ; Vol. 11, No. 8.
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    Harrison, E, Truman, J, Wright, R, Spiers, AJ, Paterson, S & Brockhurst, MA 2015, 'Plasmid carriage can limit bacteria–phage coevolution', Biology Letters, vol. 11, no. 8, 20150361. https://doi.org/10.1098/rsbl.2015.0361

    Plasmid carriage can limit bacteria–phage coevolution. / Harrison, Ellie; Truman, Julie; Wright, Rosanna; Spiers, Andrew J.; Paterson, Steve; Brockhurst, Michael A.

    In: Biology Letters, Vol. 11, No. 8, 20150361, 12.08.2015.

    Research output: Contribution to journalArticle

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    Harrison E, Truman J, Wright R, Spiers AJ, Paterson S, Brockhurst MA. Plasmid carriage can limit bacteria–phage coevolution. Biology Letters. 2015 Aug 12;11(8). 20150361. https://doi.org/10.1098/rsbl.2015.0361