Preparation of protein-stabilized β-carotene nanodispersions by emulsification-evaporation method

Boon Seang Chu, Sosaku Ichikawa, Sumiyo Kanafusa, Mitsutoshi Nakajima*

*Corresponding author for this work

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

This work was initiated to prepare protein-stabilized β-carotene nanodispersions using emulsification-evaporation. A pre-mix of the aqueous phase composed of a protein and hexane containing β-carotene was subjected to high-pressure homogenization using a microfluidizer. Hexane in the resulting emulsion was evaporated under reduced pressures, causing crystallization and precipitation of β-carotene inside the droplets and formation of β-carotene nanoparticles. Sodium caseinate (SC) was the most effective emulsifier among selected proteins in preparing the nanodispersion, with a monomodal β-carotene particle-size distribution and a 17-nm mean particle size. The results were confirmed by transmission-electron microscopy analysis. SC-stabilized nanodispersion also had considerably high ζ-potential (-27 mV at pH 7), suggesting that the nanodispersion was stable against particle aggregation. Increasing the SC concentration decreased the mean particle size and improved the polydispersity of the nanodispersions. Nanodispersions prepared with higher β-carotene concentrations and higher organic-phase ratios resulted in larger β-carotene particles. Although increased microfluidization pressure did not decrease particle size, it did improve the polydispersity of the nanodispersions. Repeating the microfluidization process at 140 MPa caused the nanodispersions to become polydisperse, indicating the loss of emulsifying capacity of SC due to protein denaturation.

Original languageEnglish
Pages (from-to)1053-1062
Number of pages10
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume84
Issue number11
DOIs
Publication statusPublished - Nov 2007
Externally publishedYes

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Emulsification
Carotenoids
Evaporation
Proteins
Caseins
Sodium
Particle size
Polydispersity
Hexanes
Hexane
Denaturation
Crystallization
Emulsions
Particle size analysis
Agglomeration
Nanoparticles
Transmission electron microscopy

Cite this

Chu, Boon Seang ; Ichikawa, Sosaku ; Kanafusa, Sumiyo ; Nakajima, Mitsutoshi. / Preparation of protein-stabilized β-carotene nanodispersions by emulsification-evaporation method. In: JAOCS, Journal of the American Oil Chemists' Society. 2007 ; Vol. 84, No. 11. pp. 1053-1062.
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Preparation of protein-stabilized β-carotene nanodispersions by emulsification-evaporation method. / Chu, Boon Seang; Ichikawa, Sosaku; Kanafusa, Sumiyo; Nakajima, Mitsutoshi.

In: JAOCS, Journal of the American Oil Chemists' Society, Vol. 84, No. 11, 11.2007, p. 1053-1062.

Research output: Contribution to journalArticle

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T1 - Preparation of protein-stabilized β-carotene nanodispersions by emulsification-evaporation method

AU - Chu, Boon Seang

AU - Ichikawa, Sosaku

AU - Kanafusa, Sumiyo

AU - Nakajima, Mitsutoshi

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N2 - This work was initiated to prepare protein-stabilized β-carotene nanodispersions using emulsification-evaporation. A pre-mix of the aqueous phase composed of a protein and hexane containing β-carotene was subjected to high-pressure homogenization using a microfluidizer. Hexane in the resulting emulsion was evaporated under reduced pressures, causing crystallization and precipitation of β-carotene inside the droplets and formation of β-carotene nanoparticles. Sodium caseinate (SC) was the most effective emulsifier among selected proteins in preparing the nanodispersion, with a monomodal β-carotene particle-size distribution and a 17-nm mean particle size. The results were confirmed by transmission-electron microscopy analysis. SC-stabilized nanodispersion also had considerably high ζ-potential (-27 mV at pH 7), suggesting that the nanodispersion was stable against particle aggregation. Increasing the SC concentration decreased the mean particle size and improved the polydispersity of the nanodispersions. Nanodispersions prepared with higher β-carotene concentrations and higher organic-phase ratios resulted in larger β-carotene particles. Although increased microfluidization pressure did not decrease particle size, it did improve the polydispersity of the nanodispersions. Repeating the microfluidization process at 140 MPa caused the nanodispersions to become polydisperse, indicating the loss of emulsifying capacity of SC due to protein denaturation.

AB - This work was initiated to prepare protein-stabilized β-carotene nanodispersions using emulsification-evaporation. A pre-mix of the aqueous phase composed of a protein and hexane containing β-carotene was subjected to high-pressure homogenization using a microfluidizer. Hexane in the resulting emulsion was evaporated under reduced pressures, causing crystallization and precipitation of β-carotene inside the droplets and formation of β-carotene nanoparticles. Sodium caseinate (SC) was the most effective emulsifier among selected proteins in preparing the nanodispersion, with a monomodal β-carotene particle-size distribution and a 17-nm mean particle size. The results were confirmed by transmission-electron microscopy analysis. SC-stabilized nanodispersion also had considerably high ζ-potential (-27 mV at pH 7), suggesting that the nanodispersion was stable against particle aggregation. Increasing the SC concentration decreased the mean particle size and improved the polydispersity of the nanodispersions. Nanodispersions prepared with higher β-carotene concentrations and higher organic-phase ratios resulted in larger β-carotene particles. Although increased microfluidization pressure did not decrease particle size, it did improve the polydispersity of the nanodispersions. Repeating the microfluidization process at 140 MPa caused the nanodispersions to become polydisperse, indicating the loss of emulsifying capacity of SC due to protein denaturation.

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