Progesterone significantly enhances the mobility of boar spermatozoa

Johan M. Campbell, Anne L. Savage, Oladipo Madamidola, Kshitij Tamhane, Renalyn Soriano, Ashok K. Adya, Sean G. Brown

Research output: Contribution to journalArticle

20 Downloads (Pure)

Abstract

Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4%, +100nM progesterone non-reacted = 47±10%). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner.
Original languageEnglish
Article numbere8955
Number of pages7
JournalBioDiscovery
Volume2013
Issue number9
DOIs
Publication statusPublished - 11 Dec 2013

Fingerprint

Progesterone
Spermatozoa
Cell Separation
Acrosome Reaction
Aptitude
Mibefradil
Cumulus Cells
Calcium Channel Blockers
Chemotaxis
Oocytes
Tyrosine
Western Blotting
Phosphorylation
Calcium

Cite this

Campbell, Johan M. ; Savage, Anne L. ; Madamidola, Oladipo ; Tamhane, Kshitij ; Soriano, Renalyn ; Adya, Ashok K. ; Brown, Sean G. / Progesterone significantly enhances the mobility of boar spermatozoa. In: BioDiscovery. 2013 ; Vol. 2013, No. 9.
@article{6fa1bd7be5594739b8773fe0b3960ecc,
title = "Progesterone significantly enhances the mobility of boar spermatozoa",
abstract = "Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4{\%}, +100nM progesterone non-reacted = 47±10{\%}). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner.",
author = "Campbell, {Johan M.} and Savage, {Anne L.} and Oladipo Madamidola and Kshitij Tamhane and Renalyn Soriano and Adya, {Ashok K.} and Brown, {Sean G.}",
year = "2013",
month = "12",
day = "11",
doi = "10.7750/BioDiscovery.2013.9.5",
language = "English",
volume = "2013",
journal = "BioDiscovery",
issn = "2050-2966",
number = "9",

}

Progesterone significantly enhances the mobility of boar spermatozoa. / Campbell, Johan M.; Savage, Anne L.; Madamidola, Oladipo; Tamhane, Kshitij; Soriano, Renalyn; Adya, Ashok K.; Brown, Sean G.

In: BioDiscovery, Vol. 2013, No. 9, e8955, 11.12.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Progesterone significantly enhances the mobility of boar spermatozoa

AU - Campbell, Johan M.

AU - Savage, Anne L.

AU - Madamidola, Oladipo

AU - Tamhane, Kshitij

AU - Soriano, Renalyn

AU - Adya, Ashok K.

AU - Brown, Sean G.

PY - 2013/12/11

Y1 - 2013/12/11

N2 - Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4%, +100nM progesterone non-reacted = 47±10%). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner.

AB - Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4%, +100nM progesterone non-reacted = 47±10%). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner.

U2 - 10.7750/BioDiscovery.2013.9.5

DO - 10.7750/BioDiscovery.2013.9.5

M3 - Article

VL - 2013

JO - BioDiscovery

JF - BioDiscovery

SN - 2050-2966

IS - 9

M1 - e8955

ER -

Campbell JM, Savage AL, Madamidola O, Tamhane K, Soriano R, Adya AK et al. Progesterone significantly enhances the mobility of boar spermatozoa. BioDiscovery. 2013 Dec 11;2013(9). e8955. https://doi.org/10.7750/BioDiscovery.2013.9.5