Protective effect of neferine against isoproterenol-induced cardiac toxicity

Gurusamy Lalitha, Paramasivan Poornima, Arjunan Archanah, Viswanadha Vijaya Padma*

*Corresponding author for this work

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The present study was designed to investigate the cardioprotective effect of neferine against isoproterenol-induced myocardial infarction. Neferine was given orally for 30 days, and isoproterenol was injected subcutaneously for 2 days. Histopathological examination of heart tissue of isoproterenol-treated rats showed myocardial necrosis. Biochemical analysis of isoproterenol-treated rats showed significant increase in the serum marker enzymes - creatine kinase, lactate dehydrogenase, and aspartate transaminase and increased serum glycoprotein components with a concomitant decrease in the heart tissue homogenate when compared to control. Increased lipid peroxidation and decreased antioxidants reduced glutathione, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase and altered lipid profile in serum and tissue was also recorded in the isoproterenol-treated rats, whereas the rats which received neferine pre-treatment followed by isoproterenol injection showed minimal histological changes, absence of inflammation, and a significant decrease in the serum marker enzymes and serum glycoprotein components with a concomitant increase in the heart tissue homogenate when compared to isoproterenol group. Neferine pre-treatment restored the altered biochemical parameters and lipid profile to near normal. The results of the present study showed that neferine exerts strong antioxidant property against isoproterenol-induced oxidative stress and can be used as a potent cardioprotective agent against isoproterenol-induced myocardial infarction.

Original languageEnglish
Pages (from-to)168-179
Number of pages12
JournalCardiovascular Toxicology
Volume13
Issue number2
Early online date30 Dec 2012
DOIs
Publication statusPublished - 1 Jun 2013
Externally publishedYes

Fingerprint

neferine
Isoproterenol
Toxicity
Rats
Tissue
Lipids
Glycoproteins
Antioxidants
Biomarkers
Serum
Myocardial Infarction
Cardiotonic Agents
Cardiotoxicity
Oxidative stress

Cite this

Lalitha, Gurusamy ; Poornima, Paramasivan ; Archanah, Arjunan ; Padma, Viswanadha Vijaya. / Protective effect of neferine against isoproterenol-induced cardiac toxicity. In: Cardiovascular Toxicology. 2013 ; Vol. 13, No. 2. pp. 168-179.
@article{44c9f146822f451db02763ff42ed33bf,
title = "Protective effect of neferine against isoproterenol-induced cardiac toxicity",
abstract = "The present study was designed to investigate the cardioprotective effect of neferine against isoproterenol-induced myocardial infarction. Neferine was given orally for 30 days, and isoproterenol was injected subcutaneously for 2 days. Histopathological examination of heart tissue of isoproterenol-treated rats showed myocardial necrosis. Biochemical analysis of isoproterenol-treated rats showed significant increase in the serum marker enzymes - creatine kinase, lactate dehydrogenase, and aspartate transaminase and increased serum glycoprotein components with a concomitant decrease in the heart tissue homogenate when compared to control. Increased lipid peroxidation and decreased antioxidants reduced glutathione, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase and altered lipid profile in serum and tissue was also recorded in the isoproterenol-treated rats, whereas the rats which received neferine pre-treatment followed by isoproterenol injection showed minimal histological changes, absence of inflammation, and a significant decrease in the serum marker enzymes and serum glycoprotein components with a concomitant increase in the heart tissue homogenate when compared to isoproterenol group. Neferine pre-treatment restored the altered biochemical parameters and lipid profile to near normal. The results of the present study showed that neferine exerts strong antioxidant property against isoproterenol-induced oxidative stress and can be used as a potent cardioprotective agent against isoproterenol-induced myocardial infarction.",
author = "Gurusamy Lalitha and Paramasivan Poornima and Arjunan Archanah and Padma, {Viswanadha Vijaya}",
year = "2013",
month = "6",
day = "1",
doi = "10.1007/s12012-012-9196-5",
language = "English",
volume = "13",
pages = "168--179",
journal = "Cardiovascular Toxicology",
issn = "1530-7905",
publisher = "Humana Press",
number = "2",

}

Protective effect of neferine against isoproterenol-induced cardiac toxicity. / Lalitha, Gurusamy; Poornima, Paramasivan; Archanah, Arjunan; Padma, Viswanadha Vijaya.

In: Cardiovascular Toxicology, Vol. 13, No. 2, 01.06.2013, p. 168-179.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protective effect of neferine against isoproterenol-induced cardiac toxicity

AU - Lalitha, Gurusamy

AU - Poornima, Paramasivan

AU - Archanah, Arjunan

AU - Padma, Viswanadha Vijaya

PY - 2013/6/1

Y1 - 2013/6/1

N2 - The present study was designed to investigate the cardioprotective effect of neferine against isoproterenol-induced myocardial infarction. Neferine was given orally for 30 days, and isoproterenol was injected subcutaneously for 2 days. Histopathological examination of heart tissue of isoproterenol-treated rats showed myocardial necrosis. Biochemical analysis of isoproterenol-treated rats showed significant increase in the serum marker enzymes - creatine kinase, lactate dehydrogenase, and aspartate transaminase and increased serum glycoprotein components with a concomitant decrease in the heart tissue homogenate when compared to control. Increased lipid peroxidation and decreased antioxidants reduced glutathione, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase and altered lipid profile in serum and tissue was also recorded in the isoproterenol-treated rats, whereas the rats which received neferine pre-treatment followed by isoproterenol injection showed minimal histological changes, absence of inflammation, and a significant decrease in the serum marker enzymes and serum glycoprotein components with a concomitant increase in the heart tissue homogenate when compared to isoproterenol group. Neferine pre-treatment restored the altered biochemical parameters and lipid profile to near normal. The results of the present study showed that neferine exerts strong antioxidant property against isoproterenol-induced oxidative stress and can be used as a potent cardioprotective agent against isoproterenol-induced myocardial infarction.

AB - The present study was designed to investigate the cardioprotective effect of neferine against isoproterenol-induced myocardial infarction. Neferine was given orally for 30 days, and isoproterenol was injected subcutaneously for 2 days. Histopathological examination of heart tissue of isoproterenol-treated rats showed myocardial necrosis. Biochemical analysis of isoproterenol-treated rats showed significant increase in the serum marker enzymes - creatine kinase, lactate dehydrogenase, and aspartate transaminase and increased serum glycoprotein components with a concomitant decrease in the heart tissue homogenate when compared to control. Increased lipid peroxidation and decreased antioxidants reduced glutathione, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase and altered lipid profile in serum and tissue was also recorded in the isoproterenol-treated rats, whereas the rats which received neferine pre-treatment followed by isoproterenol injection showed minimal histological changes, absence of inflammation, and a significant decrease in the serum marker enzymes and serum glycoprotein components with a concomitant increase in the heart tissue homogenate when compared to isoproterenol group. Neferine pre-treatment restored the altered biochemical parameters and lipid profile to near normal. The results of the present study showed that neferine exerts strong antioxidant property against isoproterenol-induced oxidative stress and can be used as a potent cardioprotective agent against isoproterenol-induced myocardial infarction.

U2 - 10.1007/s12012-012-9196-5

DO - 10.1007/s12012-012-9196-5

M3 - Article

C2 - 23274852

AN - SCOPUS:84880712614

VL - 13

SP - 168

EP - 179

JO - Cardiovascular Toxicology

JF - Cardiovascular Toxicology

SN - 1530-7905

IS - 2

ER -