Biofilms, and other bacterial aggregations, are of significance in both environmental microbiology and in plant and human pathogenesis. Comparative single-cell Raman spectral analysis can differentiate between planktonic bacteria and those recovered from biofilms and appears to offer a new means by which to investigate bacterial cell physiology, metabolic status, and stress under different environmental conditions.
Huang, W. E., Ude, S., & Spiers, A. J. (2007). Pseudomonas fluorescens SBW25 biofilm and planktonic cells have differentiable raman spectral profiles. Microbial Ecology, 53(3), 471-474. https://doi.org/10.1007/s00248-006-9190-1