Rapid compensatory evolution promotes the survival of conjugative plasmids

Ellie Harrison, Calvin Dytham, James P. J. Hall, David Guymer, Andrew J. Spiers, Steve Paterson, Michael A. Brockhurst

Research output: Contribution to journalArticle

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Abstract

Conjugative plasmids play a vital role in bacterial adaptation through horizontal gene transfer. Explaining how plasmids persist in host populations however is difficult, given the high costs often associated with plasmid carriage. Compensatory evolution to ameliorate this cost can rescue plasmids from extinction. In a recently published study we showed that compensatory evolution repeatedly targeted the same bacterial regulatory system, GacA/GacS, in populations of plasmid-carrying bacteria evolving across a range of selective environments. Mutations in these genes arose rapidly and completely eliminated the cost of plasmid carriage. Here we extend our analysis using an individual based model to explore the dynamics of compensatory evolution in this system. We show that mutations which ameliorate the cost of plasmid carriage can prevent both the loss of plasmids from the population and the fixation of accessory traits on the bacterial chromosome. We discuss how dependent the outcome of compensatory evolution is on the strength and availability of such mutations and the rate at which beneficial accessory traits integrate on the host chromosome.
Original languageEnglish
Article numbere1179074
Number of pages6
JournalMobile Genetic Elements
Volume6
Issue number3
Early online date4 May 2016
DOIs
Publication statusPublished - 4 May 2016

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Plasmids
Costs and Cost Analysis
Bacterial Chromosomes
Population
Horizontal Gene Transfer
Mutation
Mutation Rate
Chromosomes
Bacteria
Genes

Cite this

Harrison, E., Dytham, C., Hall, J. P. J., Guymer, D., Spiers, A. J., Paterson, S., & Brockhurst, M. A. (2016). Rapid compensatory evolution promotes the survival of conjugative plasmids. Mobile Genetic Elements, 6(3), [e1179074]. https://doi.org/10.1080/2159256X.2016.1179074
Harrison, Ellie ; Dytham, Calvin ; Hall, James P. J. ; Guymer, David ; Spiers, Andrew J. ; Paterson, Steve ; Brockhurst, Michael A. / Rapid compensatory evolution promotes the survival of conjugative plasmids. In: Mobile Genetic Elements. 2016 ; Vol. 6, No. 3.
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Harrison, E, Dytham, C, Hall, JPJ, Guymer, D, Spiers, AJ, Paterson, S & Brockhurst, MA 2016, 'Rapid compensatory evolution promotes the survival of conjugative plasmids', Mobile Genetic Elements, vol. 6, no. 3, e1179074. https://doi.org/10.1080/2159256X.2016.1179074

Rapid compensatory evolution promotes the survival of conjugative plasmids. / Harrison, Ellie; Dytham, Calvin; Hall, James P. J.; Guymer, David; Spiers, Andrew J.; Paterson, Steve; Brockhurst, Michael A.

In: Mobile Genetic Elements, Vol. 6, No. 3, e1179074, 04.05.2016.

Research output: Contribution to journalArticle

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AU - Harrison, Ellie

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AU - Brockhurst, Michael A.

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