RepFIB

a basic replicon of large plasmids

M. D. Gibbs, A. J. Spiers, P. L. Bergquist

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The distribution of the RepFIB replicon among a total of 20 plasmid incompatibility groups was determined using Southern blot and polymerase chain reaction analysis. The presence of the replicon was confirmed in 25 of 55 plasmids tested. The majority of plasmids carrying RepFIB are from the IncF incompatibility groups, but one plasmid from IncI and one plasmid from IncP also possess the replicon. Seven different examples of RepFIB were sequenced in the minimal replicon region to obtain 1525 bp of sequence information covering the repA gene and flanking repeat regions for comparison. An analysis of these sequences plus three sequences previously reported showed almost perfect conservation of the predicted protein sequence of RepA and of the flanking DNA repeats. DNA sequence data were analyzed using maximum parsimony techniques to describe the possible evolutionary relationships of the 10 examples of RepFIB.

Original languageEnglish
Pages (from-to)165-179
Number of pages15
JournalPlasmid
Volume29
Issue number3
DOIs
Publication statusPublished - May 1993
Externally publishedYes

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Replicon
Plasmids
Southern Blotting
Sequence Analysis
Polymerase Chain Reaction
DNA
Genes
Proteins

Cite this

Gibbs, M. D. ; Spiers, A. J. ; Bergquist, P. L. / RepFIB : a basic replicon of large plasmids. In: Plasmid. 1993 ; Vol. 29, No. 3. pp. 165-179.
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Gibbs, MD, Spiers, AJ & Bergquist, PL 1993, 'RepFIB: a basic replicon of large plasmids', Plasmid, vol. 29, no. 3, pp. 165-179. https://doi.org/10.1006/plas.1993.1020

RepFIB : a basic replicon of large plasmids. / Gibbs, M. D.; Spiers, A. J.; Bergquist, P. L.

In: Plasmid, Vol. 29, No. 3, 05.1993, p. 165-179.

Research output: Contribution to journalArticle

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T1 - RepFIB

T2 - a basic replicon of large plasmids

AU - Gibbs, M. D.

AU - Spiers, A. J.

AU - Bergquist, P. L.

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