Root phenomics of crops: opportunities and challenges

Peter J. Gregory, A. Glyn Bengough, Dmitri V. Grinev, Sonja Schmidt, W. (Bill) T. B. Thomas, Tobias Wojciechowski, Iain M. Young

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Reliable techniques for screening large numbers of plants for root traits are still being developed, but include aeroponic, hydroponic and agar plate systems. Coupled with digital cameras and image analysis software, these systems permit the rapid measurement of root numbers, length and diameter in moderate (typically <1000) numbers of plants. Usually such systems are employed with relatively small seedlings, and information is recorded in 2D. Recent developments in X-ray microtomography have facilitated 3D non-invasive measurement of small root systems grown in solid media, allowing angular distributions to be obtained in addition to numbers and length. However, because of the time taken to scan samples, only a small number can be screened (typically <10 per day, not including analysis time of the large spatial datasets generated) and, depending on sample size, limited resolution may mean that fine roots remain unresolved. Although agar plates allow differences between lines and genotypes to be discerned in young seedlings, the rank order may not be the same when the same materials are grown in solid media. For example, root length of dwarfing wheat (Triticum aestivum L.) lines grown on agar plates was increased by ~40% relative to wild-type and semi-dwarfing lines, but in a sandy loam soil under well watered conditions it was decreased by 24–33%. Such differences in ranking suggest that significant soil environment–genotype interactions are occurring. Developments in instruments and software mean that a combination of high-throughput simple screens and more in-depth examination of root–soil interactions is becoming viable.
Original languageEnglish
Pages (from-to)922-929
Number of pages8
JournalFunctional Plant Biology
Volume36
Issue number11
DOIs
Publication statusPublished - 2009

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dwarfing
agar
crops
micro-computed tomography
seedlings
spatial data
sandy loam soils
hydroponics
cameras
root systems
Triticum aestivum
image analysis
screening
sampling
wheat
genotype
phenomics
soil
methodology
aeroponics

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Gregory, P. J., Bengough, A. G., Grinev, D. V., Schmidt, S., Thomas, W. B. T. B., Wojciechowski, T., & Young, I. M. (2009). Root phenomics of crops: opportunities and challenges. Functional Plant Biology, 36(11), 922-929. https://doi.org/10.1071/FP09150
Gregory, Peter J. ; Bengough, A. Glyn ; Grinev, Dmitri V. ; Schmidt, Sonja ; Thomas, W. (Bill) T. B. ; Wojciechowski, Tobias ; Young, Iain M. / Root phenomics of crops : opportunities and challenges. In: Functional Plant Biology. 2009 ; Vol. 36, No. 11. pp. 922-929.
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Gregory, PJ, Bengough, AG, Grinev, DV, Schmidt, S, Thomas, WBTB, Wojciechowski, T & Young, IM 2009, 'Root phenomics of crops: opportunities and challenges', Functional Plant Biology, vol. 36, no. 11, pp. 922-929. https://doi.org/10.1071/FP09150

Root phenomics of crops : opportunities and challenges. / Gregory, Peter J.; Bengough, A. Glyn; Grinev, Dmitri V.; Schmidt, Sonja; Thomas, W. (Bill) T. B.; Wojciechowski, Tobias; Young, Iain M.

In: Functional Plant Biology, Vol. 36, No. 11, 2009, p. 922-929.

Research output: Contribution to journalArticle

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T2 - opportunities and challenges

AU - Gregory, Peter J.

AU - Bengough, A. Glyn

AU - Grinev, Dmitri V.

AU - Schmidt, Sonja

AU - Thomas, W. (Bill) T. B.

AU - Wojciechowski, Tobias

AU - Young, Iain M.

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DO - 10.1071/FP09150

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JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

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ER -

Gregory PJ, Bengough AG, Grinev DV, Schmidt S, Thomas WBTB, Wojciechowski T et al. Root phenomics of crops: opportunities and challenges. Functional Plant Biology. 2009;36(11):922-929. https://doi.org/10.1071/FP09150