Separation of tocopherols and tocotrienols from palm fatty acid distillate using hydrolysis-neutralization-adsorption chromatography method

B. S. Chu, B. S. Baharin*, S. Y. Quek, Y. B.Che Man

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Vitamin E from palm fatty acid distillate (PFAD) was concentrated in this study by removing the extraneous matters, especially free fatty acids (FFA) and acylglycerols. Original PFAD used in this study contained 85 and 13.7% of FFA and acylglycerols, respectively. Acylglycerols in PFAD were first hydrolyzed using commercially available immobilized Candida antarctica lipase at 60C for 6 h. The fatty acids liberated, together with the existing FFA were then removed by neutralization. Hydrolysis followed by neutralization step concentrated vitamin E from an initial amount of 0.37 to 4.32%. Hydrolysis was an important step as without hydrolysis, neutralization could only concentrate vitamin E to 1.80%. Oil extracted from hydrolyzed and neutralized PFAD was then subjected to a normal-phase silica adsorption chromatography. The column was first eluted with hexane followed by isopropanol. A large amount of acylglycerols was removed during the first elution, while vitamin E was desorbed by isopropanol, with a recovery of >90%. Vitamin E concentration after hydrolysis, neutralization and adsorption chromatography was 15.2%. The method described in this study offers an alternative for the existing separation methods. It could be applied as one of a series of steps in producing high-purity vitamin E concentrates.

Original languageEnglish
Pages (from-to)141-152
Number of pages12
JournalJournal of Food Lipids
Volume10
Issue number2
DOIs
Publication statusPublished - Jun 2003
Externally publishedYes

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Tocotrienols
tocotrienols
Tocopherols
Chromatography
Vitamin E
tocopherols
neutralization
Adsorption
vitamin E
Glycerides
Hydrolysis
chromatography
adsorption
Fatty Acids
acylglycerols
hydrolysis
fatty acids
Nonesterified Fatty Acids
free fatty acids
2-Propanol

Cite this

@article{c9a844851e014fcd88ada06105459898,
title = "Separation of tocopherols and tocotrienols from palm fatty acid distillate using hydrolysis-neutralization-adsorption chromatography method",
abstract = "Vitamin E from palm fatty acid distillate (PFAD) was concentrated in this study by removing the extraneous matters, especially free fatty acids (FFA) and acylglycerols. Original PFAD used in this study contained 85 and 13.7{\%} of FFA and acylglycerols, respectively. Acylglycerols in PFAD were first hydrolyzed using commercially available immobilized Candida antarctica lipase at 60C for 6 h. The fatty acids liberated, together with the existing FFA were then removed by neutralization. Hydrolysis followed by neutralization step concentrated vitamin E from an initial amount of 0.37 to 4.32{\%}. Hydrolysis was an important step as without hydrolysis, neutralization could only concentrate vitamin E to 1.80{\%}. Oil extracted from hydrolyzed and neutralized PFAD was then subjected to a normal-phase silica adsorption chromatography. The column was first eluted with hexane followed by isopropanol. A large amount of acylglycerols was removed during the first elution, while vitamin E was desorbed by isopropanol, with a recovery of >90{\%}. Vitamin E concentration after hydrolysis, neutralization and adsorption chromatography was 15.2{\%}. The method described in this study offers an alternative for the existing separation methods. It could be applied as one of a series of steps in producing high-purity vitamin E concentrates.",
author = "Chu, {B. S.} and Baharin, {B. S.} and Quek, {S. Y.} and Man, {Y. B.Che}",
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Separation of tocopherols and tocotrienols from palm fatty acid distillate using hydrolysis-neutralization-adsorption chromatography method. / Chu, B. S.; Baharin, B. S.; Quek, S. Y.; Man, Y. B.Che.

In: Journal of Food Lipids, Vol. 10, No. 2, 06.2003, p. 141-152.

Research output: Contribution to journalArticle

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AU - Chu, B. S.

AU - Baharin, B. S.

AU - Quek, S. Y.

AU - Man, Y. B.Che

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