Scambi di cromatidi fratelli e aberrazioni cromosomiche indotte dalla fusaproliferina nei cromosomi mitotici della capra (Capra hircus L.)

Translated title of the contribution: Sister chromatid exchanges (SCEs) and chromosome aberrations induced by fusaproliferin in mitotic chromosomes of the goat (Capra hircus L.)

D Di Berardino, G De Rosa, V Fogliano, A Fiore, A Ritieni

Research output: Contribution to journalArticlepeer-review

Abstract

Severe teratogenic effects have been reported on chick embryos treated with 1-5 mM of fusaproliferin, a new toxic metabolite extracted from Fusarium proliferatum infected maize cultures. In order to understand the mode of action of this mycotoxin, sister chromatid exchanges (SCEs) and chromatid/chromosome breaks were studied on mitotic chromosomes of the goat (Capra hircus L.). Peripheral lymphocytes of the goat were treated with 0.5 microng/ml of BrdU as control and with a constant dose of 0.5 microng/ml of BrdU plus increasing concentrations of fusaproliferin, i.e. 0.05, 0.1, 0.2, 0.4 and 0.8 microng/ml. The results demonstrated SCE and chromatid breaks inducing effects of the substance. The mean rates of SCEs per cell were: 6.5 +- 1.2 in the BrdU-control cells, 7.6 +- 2.2 and 10.0 +- 3.1, respectively, at the lowest and highest dosages of fusaproliferin; the mean rates of chromatid/chromosome breaks per cell were: 0.53 +- 0.72 in the BrdU-control cells, 0.69 +- 0.78 and 0.93 +- 0.8, respectively, at the lowest and highest dosages of fusaproliferin. Chromatid breaks were preferentially induced compared to chromosome ones: in the BrdU-control cells (78 vs 22%, respectively), 82 vs 18% and 92 vs 8% at the lowest and highest dosages of fusaproliferin. The pronounced clastogenic action and the SCE-inducing effects demonstrated by fusaproliferin may, at least partially, explain the high level of teratogenic effects observed on chick embryos.
Translated title of the contributionSister chromatid exchanges (SCEs) and chromosome aberrations induced by fusaproliferin in mitotic chromosomes of the goat (Capra hircus L.)
Original languageItalian
Pages (from-to)135-143
Number of pages9
JournalAnnali della Facoltà di Scienze Agrarie della Università degli studi di Napoli. Portici
Volume1
Publication statusPublished - 2004
Externally publishedYes

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