Scambi di cromatidi fratelli e aberrazioni cromosomiche indotte dalla fusaproliferina nei cromosomi mitotici della capra (Capra hircus L.)

Translated title of the contribution: Sister chromatid exchanges (SCEs) and chromosome aberrations induced by fusaproliferin in mitotic chromosomes of the goat (Capra hircus L.)

D Di Berardino, G De Rosa, V Fogliano, A Fiore, A Ritieni

Research output: Contribution to journalArticle

Abstract

Severe teratogenic effects have been reported on chick embryos treated with 1-5 mM of fusaproliferin, a new toxic metabolite extracted from Fusarium proliferatum infected maize cultures. In order to understand the mode of action of this mycotoxin, sister chromatid exchanges (SCEs) and chromatid/chromosome breaks were studied on mitotic chromosomes of the goat (Capra hircus L.). Peripheral lymphocytes of the goat were treated with 0.5 microng/ml of BrdU as control and with a constant dose of 0.5 microng/ml of BrdU plus increasing concentrations of fusaproliferin, i.e. 0.05, 0.1, 0.2, 0.4 and 0.8 microng/ml. The results demonstrated SCE and chromatid breaks inducing effects of the substance. The mean rates of SCEs per cell were: 6.5 +- 1.2 in the BrdU-control cells, 7.6 +- 2.2 and 10.0 +- 3.1, respectively, at the lowest and highest dosages of fusaproliferin; the mean rates of chromatid/chromosome breaks per cell were: 0.53 +- 0.72 in the BrdU-control cells, 0.69 +- 0.78 and 0.93 +- 0.8, respectively, at the lowest and highest dosages of fusaproliferin. Chromatid breaks were preferentially induced compared to chromosome ones: in the BrdU-control cells (78 vs 22%, respectively), 82 vs 18% and 92 vs 8% at the lowest and highest dosages of fusaproliferin. The pronounced clastogenic action and the SCE-inducing effects demonstrated by fusaproliferin may, at least partially, explain the high level of teratogenic effects observed on chick embryos.
Original languageItalian
Pages (from-to)135-143
Number of pages9
JournalAnnali della Facoltà di Scienze Agrarie della Università degli studi di Napoli. Portici
Volume1
Publication statusPublished - 2004
Externally publishedYes

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chromosome aberrations
crossing over
chromatids
goats
chromosomes
chromosome breakage
dosage
cells
embryo (animal)
chicks
Fusarium proliferatum
mycotoxins
mechanism of action
lymphocytes
Capra hircus
metabolites
corn

Cite this

@article{0d9e44cf07e7427c88972c451a394032,
title = "Scambi di cromatidi fratelli e aberrazioni cromosomiche indotte dalla fusaproliferina nei cromosomi mitotici della capra (Capra hircus L.)",
abstract = "Severe teratogenic effects have been reported on chick embryos treated with 1-5 mM of fusaproliferin, a new toxic metabolite extracted from Fusarium proliferatum infected maize cultures. In order to understand the mode of action of this mycotoxin, sister chromatid exchanges (SCEs) and chromatid/chromosome breaks were studied on mitotic chromosomes of the goat (Capra hircus L.). Peripheral lymphocytes of the goat were treated with 0.5 microng/ml of BrdU as control and with a constant dose of 0.5 microng/ml of BrdU plus increasing concentrations of fusaproliferin, i.e. 0.05, 0.1, 0.2, 0.4 and 0.8 microng/ml. The results demonstrated SCE and chromatid breaks inducing effects of the substance. The mean rates of SCEs per cell were: 6.5 +- 1.2 in the BrdU-control cells, 7.6 +- 2.2 and 10.0 +- 3.1, respectively, at the lowest and highest dosages of fusaproliferin; the mean rates of chromatid/chromosome breaks per cell were: 0.53 +- 0.72 in the BrdU-control cells, 0.69 +- 0.78 and 0.93 +- 0.8, respectively, at the lowest and highest dosages of fusaproliferin. Chromatid breaks were preferentially induced compared to chromosome ones: in the BrdU-control cells (78 vs 22{\%}, respectively), 82 vs 18{\%} and 92 vs 8{\%} at the lowest and highest dosages of fusaproliferin. The pronounced clastogenic action and the SCE-inducing effects demonstrated by fusaproliferin may, at least partially, explain the high level of teratogenic effects observed on chick embryos.",
author = "{Di Berardino}, D and Rosa, {G De} and V Fogliano and A Fiore and A Ritieni",
year = "2004",
language = "Italian",
volume = "1",
pages = "135--143",
journal = "Annali della Facolt{\`a} di Scienze Agrarie della Universit{\`a} degli studi di Napoli. Portici",
issn = "0365-799X",
publisher = "Pierro e Veraldi",

}

TY - JOUR

T1 - Scambi di cromatidi fratelli e aberrazioni cromosomiche indotte dalla fusaproliferina nei cromosomi mitotici della capra (Capra hircus L.)

AU - Di Berardino, D

AU - Rosa, G De

AU - Fogliano, V

AU - Fiore, A

AU - Ritieni, A

PY - 2004

Y1 - 2004

N2 - Severe teratogenic effects have been reported on chick embryos treated with 1-5 mM of fusaproliferin, a new toxic metabolite extracted from Fusarium proliferatum infected maize cultures. In order to understand the mode of action of this mycotoxin, sister chromatid exchanges (SCEs) and chromatid/chromosome breaks were studied on mitotic chromosomes of the goat (Capra hircus L.). Peripheral lymphocytes of the goat were treated with 0.5 microng/ml of BrdU as control and with a constant dose of 0.5 microng/ml of BrdU plus increasing concentrations of fusaproliferin, i.e. 0.05, 0.1, 0.2, 0.4 and 0.8 microng/ml. The results demonstrated SCE and chromatid breaks inducing effects of the substance. The mean rates of SCEs per cell were: 6.5 +- 1.2 in the BrdU-control cells, 7.6 +- 2.2 and 10.0 +- 3.1, respectively, at the lowest and highest dosages of fusaproliferin; the mean rates of chromatid/chromosome breaks per cell were: 0.53 +- 0.72 in the BrdU-control cells, 0.69 +- 0.78 and 0.93 +- 0.8, respectively, at the lowest and highest dosages of fusaproliferin. Chromatid breaks were preferentially induced compared to chromosome ones: in the BrdU-control cells (78 vs 22%, respectively), 82 vs 18% and 92 vs 8% at the lowest and highest dosages of fusaproliferin. The pronounced clastogenic action and the SCE-inducing effects demonstrated by fusaproliferin may, at least partially, explain the high level of teratogenic effects observed on chick embryos.

AB - Severe teratogenic effects have been reported on chick embryos treated with 1-5 mM of fusaproliferin, a new toxic metabolite extracted from Fusarium proliferatum infected maize cultures. In order to understand the mode of action of this mycotoxin, sister chromatid exchanges (SCEs) and chromatid/chromosome breaks were studied on mitotic chromosomes of the goat (Capra hircus L.). Peripheral lymphocytes of the goat were treated with 0.5 microng/ml of BrdU as control and with a constant dose of 0.5 microng/ml of BrdU plus increasing concentrations of fusaproliferin, i.e. 0.05, 0.1, 0.2, 0.4 and 0.8 microng/ml. The results demonstrated SCE and chromatid breaks inducing effects of the substance. The mean rates of SCEs per cell were: 6.5 +- 1.2 in the BrdU-control cells, 7.6 +- 2.2 and 10.0 +- 3.1, respectively, at the lowest and highest dosages of fusaproliferin; the mean rates of chromatid/chromosome breaks per cell were: 0.53 +- 0.72 in the BrdU-control cells, 0.69 +- 0.78 and 0.93 +- 0.8, respectively, at the lowest and highest dosages of fusaproliferin. Chromatid breaks were preferentially induced compared to chromosome ones: in the BrdU-control cells (78 vs 22%, respectively), 82 vs 18% and 92 vs 8% at the lowest and highest dosages of fusaproliferin. The pronounced clastogenic action and the SCE-inducing effects demonstrated by fusaproliferin may, at least partially, explain the high level of teratogenic effects observed on chick embryos.

M3 - Article

VL - 1

SP - 135

EP - 143

JO - Annali della Facoltà di Scienze Agrarie della Università degli studi di Napoli. Portici

JF - Annali della Facoltà di Scienze Agrarie della Università degli studi di Napoli. Portici

SN - 0365-799X

ER -