Species specificity in avian sperm: perivitelline interaction

Sarah G Stewart, Nina Bausek, Franz Wohlrab, Wolfgang J Schneider, A. Janet Horrocks, Graham J Wishart

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    The interaction of chicken spermatozoa with the inner perivitelline layer from different avian species in vitro during a 5 min co-incubation was measured as the number of points of hydrolysis produced per unit area of inner perivitelline layer. The average degree of interaction, as a proportion of that between chicken spermatozoa and their homologous inner perivitelline layer, was: equal to or greater than 100% within Galliformes (chicken, turkey, quail, pheasant, peafowl and guineafowl); 44% within Anseriformes (goose, duck); and less than 30% in Passeriformes (Zebra Finch) and Columbiformes (collared-dove). The homologue of the putative chicken sperm-binding proteins, chicken ZP1 and ZP3, were identified by Western blotting with anti-chicken ZP1/ZP3 antibody in the perivitelline layers of all species. The functional cross-reactivity between chicken spermatozoa and heterologous inner perivitelline layer appeared to be linked to known phylogenetic distance between the species, although it was not related to the relative affinity of the different ZP3 homologues for anti-chicken ZP3. This work demonstrates that sperm interaction with the egg investment does not represent such a stringent species-specific barrier in birds as it does in mammals and marine invertebrates. This may be a factor in the frequency of hybrid production in birds.

    Original languageEnglish
    Pages (from-to)657-663
    Number of pages7
    JournalComparative biochemistry and physiology. Part A, Molecular & integrative physiology
    Volume137
    Issue number4
    DOIs
    Publication statusPublished - Apr 2004

    Fingerprint

    Species Specificity
    Birds
    Spermatozoa
    Chickens
    Mammals
    Hydrolysis
    Antibodies
    Columbiformes
    Anseriformes
    Galliformes
    Passeriformes
    Sperm-Ovum Interactions
    Finches
    Geese
    Quail
    Ducks
    Equidae
    Invertebrates
    Western Blotting
    spermadhesin

    Cite this

    Stewart, Sarah G ; Bausek, Nina ; Wohlrab, Franz ; Schneider, Wolfgang J ; Horrocks, A. Janet ; Wishart, Graham J. / Species specificity in avian sperm : perivitelline interaction. In: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 2004 ; Vol. 137, No. 4. pp. 657-663.
    @article{a9616b159b024e098f2a7d386c1528b3,
    title = "Species specificity in avian sperm: perivitelline interaction",
    abstract = "The interaction of chicken spermatozoa with the inner perivitelline layer from different avian species in vitro during a 5 min co-incubation was measured as the number of points of hydrolysis produced per unit area of inner perivitelline layer. The average degree of interaction, as a proportion of that between chicken spermatozoa and their homologous inner perivitelline layer, was: equal to or greater than 100{\%} within Galliformes (chicken, turkey, quail, pheasant, peafowl and guineafowl); 44{\%} within Anseriformes (goose, duck); and less than 30{\%} in Passeriformes (Zebra Finch) and Columbiformes (collared-dove). The homologue of the putative chicken sperm-binding proteins, chicken ZP1 and ZP3, were identified by Western blotting with anti-chicken ZP1/ZP3 antibody in the perivitelline layers of all species. The functional cross-reactivity between chicken spermatozoa and heterologous inner perivitelline layer appeared to be linked to known phylogenetic distance between the species, although it was not related to the relative affinity of the different ZP3 homologues for anti-chicken ZP3. This work demonstrates that sperm interaction with the egg investment does not represent such a stringent species-specific barrier in birds as it does in mammals and marine invertebrates. This may be a factor in the frequency of hybrid production in birds.",
    author = "Stewart, {Sarah G} and Nina Bausek and Franz Wohlrab and Schneider, {Wolfgang J} and Horrocks, {A. Janet} and Wishart, {Graham J}",
    year = "2004",
    month = "4",
    doi = "10.1016/j.cbpb.2004.01.027",
    language = "English",
    volume = "137",
    pages = "657--663",
    journal = "Comparative Biochemistry & Physiology; A: Comparative Physiology",
    issn = "1095-6433",
    publisher = "Elsevier Inc.",
    number = "4",

    }

    Species specificity in avian sperm : perivitelline interaction. / Stewart, Sarah G; Bausek, Nina; Wohlrab, Franz; Schneider, Wolfgang J; Horrocks, A. Janet; Wishart, Graham J.

    In: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol. 137, No. 4, 04.2004, p. 657-663.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Species specificity in avian sperm

    T2 - perivitelline interaction

    AU - Stewart, Sarah G

    AU - Bausek, Nina

    AU - Wohlrab, Franz

    AU - Schneider, Wolfgang J

    AU - Horrocks, A. Janet

    AU - Wishart, Graham J

    PY - 2004/4

    Y1 - 2004/4

    N2 - The interaction of chicken spermatozoa with the inner perivitelline layer from different avian species in vitro during a 5 min co-incubation was measured as the number of points of hydrolysis produced per unit area of inner perivitelline layer. The average degree of interaction, as a proportion of that between chicken spermatozoa and their homologous inner perivitelline layer, was: equal to or greater than 100% within Galliformes (chicken, turkey, quail, pheasant, peafowl and guineafowl); 44% within Anseriformes (goose, duck); and less than 30% in Passeriformes (Zebra Finch) and Columbiformes (collared-dove). The homologue of the putative chicken sperm-binding proteins, chicken ZP1 and ZP3, were identified by Western blotting with anti-chicken ZP1/ZP3 antibody in the perivitelline layers of all species. The functional cross-reactivity between chicken spermatozoa and heterologous inner perivitelline layer appeared to be linked to known phylogenetic distance between the species, although it was not related to the relative affinity of the different ZP3 homologues for anti-chicken ZP3. This work demonstrates that sperm interaction with the egg investment does not represent such a stringent species-specific barrier in birds as it does in mammals and marine invertebrates. This may be a factor in the frequency of hybrid production in birds.

    AB - The interaction of chicken spermatozoa with the inner perivitelline layer from different avian species in vitro during a 5 min co-incubation was measured as the number of points of hydrolysis produced per unit area of inner perivitelline layer. The average degree of interaction, as a proportion of that between chicken spermatozoa and their homologous inner perivitelline layer, was: equal to or greater than 100% within Galliformes (chicken, turkey, quail, pheasant, peafowl and guineafowl); 44% within Anseriformes (goose, duck); and less than 30% in Passeriformes (Zebra Finch) and Columbiformes (collared-dove). The homologue of the putative chicken sperm-binding proteins, chicken ZP1 and ZP3, were identified by Western blotting with anti-chicken ZP1/ZP3 antibody in the perivitelline layers of all species. The functional cross-reactivity between chicken spermatozoa and heterologous inner perivitelline layer appeared to be linked to known phylogenetic distance between the species, although it was not related to the relative affinity of the different ZP3 homologues for anti-chicken ZP3. This work demonstrates that sperm interaction with the egg investment does not represent such a stringent species-specific barrier in birds as it does in mammals and marine invertebrates. This may be a factor in the frequency of hybrid production in birds.

    U2 - 10.1016/j.cbpb.2004.01.027

    DO - 10.1016/j.cbpb.2004.01.027

    M3 - Article

    C2 - 15123173

    VL - 137

    SP - 657

    EP - 663

    JO - Comparative Biochemistry & Physiology; A: Comparative Physiology

    JF - Comparative Biochemistry & Physiology; A: Comparative Physiology

    SN - 1095-6433

    IS - 4

    ER -