Studying protein-DNA binding by dynamic light scattering

N. Zh Zhelev, R. S. Buckle, D. Snyder, P. J. Marsh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

Original languageEnglish
Pages (from-to)199-203
Number of pages5
JournalCellular and Molecular Biology Letters
Volume1
Issue number2
Publication statusPublished - Jun 1996
Externally publishedYes

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DNA-Binding Proteins
Dynamic light scattering
DNA
Dimerization
Hydrodynamics
Transcription Factors
Light
Light scattering
Proteins
Dynamic Light Scattering
Experiments

Cite this

Zhelev, N. Z., Buckle, R. S., Snyder, D., & Marsh, P. J. (1996). Studying protein-DNA binding by dynamic light scattering. Cellular and Molecular Biology Letters, 1(2), 199-203.
Zhelev, N. Zh ; Buckle, R. S. ; Snyder, D. ; Marsh, P. J. / Studying protein-DNA binding by dynamic light scattering. In: Cellular and Molecular Biology Letters. 1996 ; Vol. 1, No. 2. pp. 199-203.
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Zhelev, NZ, Buckle, RS, Snyder, D & Marsh, PJ 1996, 'Studying protein-DNA binding by dynamic light scattering', Cellular and Molecular Biology Letters, vol. 1, no. 2, pp. 199-203.

Studying protein-DNA binding by dynamic light scattering. / Zhelev, N. Zh; Buckle, R. S.; Snyder, D.; Marsh, P. J.

In: Cellular and Molecular Biology Letters, Vol. 1, No. 2, 06.1996, p. 199-203.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Studying protein-DNA binding by dynamic light scattering

AU - Zhelev, N. Zh

AU - Buckle, R. S.

AU - Snyder, D.

AU - Marsh, P. J.

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Y1 - 1996/6

N2 - Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

AB - Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

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JO - Cellular and Molecular Biology Letters

JF - Cellular and Molecular Biology Letters

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Zhelev NZ, Buckle RS, Snyder D, Marsh PJ. Studying protein-DNA binding by dynamic light scattering. Cellular and Molecular Biology Letters. 1996 Jun;1(2):199-203.