Studying protein-DNA binding by dynamic light scattering

N. Zh Zhelev, R. S. Buckle, D. Snyder, P. J. Marsh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

Original languageEnglish
Pages (from-to)199-203
Number of pages5
JournalCellular and Molecular Biology Letters
Volume1
Issue number2
Publication statusPublished - Jun 1996
Externally publishedYes

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Zhelev, N. Z., Buckle, R. S., Snyder, D., & Marsh, P. J. (1996). Studying protein-DNA binding by dynamic light scattering. Cellular and Molecular Biology Letters, 1(2), 199-203.