Studying protein-DNA binding by dynamic light scattering

N. Zh Zhelev; R. S. Buckle; D. Snyder; P. J. Marsh

Studying protein-DNA binding by dynamic light scattering

N. Zh Zhelev, R. S. Buckle, D. Snyder, P. J. Marsh

Research output: Contribution to journal › Article › peer-review

Abstract

Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (R_h) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

Original language	English
Pages (from-to)	199-203
Number of pages	5
Journal	Cellular and Molecular Biology Letters
Volume	1
Issue number	2
Publication status	Published - Jun 1996
Externally published	Yes

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Cite this

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title = "Studying protein-DNA binding by dynamic light scattering",

abstract = "Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.",

author = "Zhelev, \{N. Zh\} and Buckle, \{R. S.\} and D. Snyder and Marsh, \{P. J.\}",

year = "1996",

month = jun,

language = "English",

volume = "1",

pages = "199--203",

journal = "Cellular and Molecular Biology Letters",

issn = "1425-8153",

publisher = "BioMed Central",

number = "2",

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TY - JOUR

T1 - Studying protein-DNA binding by dynamic light scattering

AU - Zhelev, N. Zh

AU - Buckle, R. S.

AU - Snyder, D.

AU - Marsh, P. J.

PY - 1996/6

Y1 - 1996/6

N2 - Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

AB - Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.

M3 - Article

AN - SCOPUS:3142632242

SN - 1425-8153

VL - 1

SP - 199

EP - 203

JO - Cellular and Molecular Biology Letters

JF - Cellular and Molecular Biology Letters

IS - 2

ER -

Studying protein-DNA binding by dynamic light scattering

Abstract

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