Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid

Bhavna Ahluwalia; John H. Duffus; Linda J. Paterson; Graeme M. Walker

doi:10.1099/00221287-106-2-261

Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid

Bhavna Ahluwalia, John H. Duffus, Linda J. Paterson, Graeme M. Walker

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

When cultures of Schizosaccharomyces pombe growing exponentially in a minimal medium were treated with 50 mM-EDTA for 1 h, nucleic acid synthesis was inhibited but protein synthesis was not. On transfer to fresh medium, 50% of the cells divided synchronously. These phenomena may be explained on the basis of reduced availability of Mg²⁺ following chelation with EDTA.

Original language	English
Pages (from-to)	261-264
Number of pages	4
Journal	Journal of General Microbiology
Volume	106
Issue number	2
DOIs	https://doi.org/10.1099/00221287-106-2-261
Publication status	Published - 1 Jun 1978

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title = "Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid",

abstract = "When cultures of Schizosaccharomyces pombe growing exponentially in a minimal medium were treated with 50 mM-EDTA for 1 h, nucleic acid synthesis was inhibited but protein synthesis was not. On transfer to fresh medium, 50% of the cells divided synchronously. These phenomena may be explained on the basis of reduced availability of Mg2+ following chelation with EDTA.",

author = "Bhavna Ahluwalia and Duffus, {John H.} and Paterson, {Linda J.} and Walker, {Graeme M.}",

year = "1978",

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doi = "10.1099/00221287-106-2-261",

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T1 - Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid

AU - Ahluwalia, Bhavna

AU - Duffus, John H.

AU - Paterson, Linda J.

AU - Walker, Graeme M.

PY - 1978/6/1

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N2 - When cultures of Schizosaccharomyces pombe growing exponentially in a minimal medium were treated with 50 mM-EDTA for 1 h, nucleic acid synthesis was inhibited but protein synthesis was not. On transfer to fresh medium, 50% of the cells divided synchronously. These phenomena may be explained on the basis of reduced availability of Mg2+ following chelation with EDTA.

AB - When cultures of Schizosaccharomyces pombe growing exponentially in a minimal medium were treated with 50 mM-EDTA for 1 h, nucleic acid synthesis was inhibited but protein synthesis was not. On transfer to fresh medium, 50% of the cells divided synchronously. These phenomena may be explained on the basis of reduced availability of Mg2+ following chelation with EDTA.

U2 - 10.1099/00221287-106-2-261

DO - 10.1099/00221287-106-2-261

M3 - Article

VL - 106

SP - 261

EP - 264

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 2

ER -