Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid

Bhavna Ahluwalia; John H. Duffus; Linda J. Paterson; Graeme M. Walker

doi:10.1099/00221287-106-2-261

Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid

Bhavna Ahluwalia, John H. Duffus, Linda J. Paterson, Graeme M. Walker

Abertay University

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

When cultures of Schizosaccharomyces pombe growing exponentially in a minimal medium were treated with 50 mM-EDTA for 1 h, nucleic acid synthesis was inhibited but protein synthesis was not. On transfer to fresh medium, 50% of the cells divided synchronously. These phenomena may be explained on the basis of reduced availability of Mg²⁺ following chelation with EDTA.

Original language	English
Pages (from-to)	261-264
Number of pages	4
Journal	Journal of General Microbiology
Volume	106
Issue number	2
DOIs	https://doi.org/10.1099/00221287-106-2-261
Publication status	Published - 1 Jun 1978

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Ahluwalia, B., Duffus, J. H., Paterson, L. J., & Walker, G. M. (1978). Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid. Journal of General Microbiology, 106(2), 261-264. https://doi.org/10.1099/00221287-106-2-261

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abstract = "When cultures of Schizosaccharomyces pombe growing exponentially in a minimal medium were treated with 50 mM-EDTA for 1 h, nucleic acid synthesis was inhibited but protein synthesis was not. On transfer to fresh medium, 50% of the cells divided synchronously. These phenomena may be explained on the basis of reduced availability of Mg2+ following chelation with EDTA.",

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AB - When cultures of Schizosaccharomyces pombe growing exponentially in a minimal medium were treated with 50 mM-EDTA for 1 h, nucleic acid synthesis was inhibited but protein synthesis was not. On transfer to fresh medium, 50% of the cells divided synchronously. These phenomena may be explained on the basis of reduced availability of Mg2+ following chelation with EDTA.

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10.1099/00221287-106-2-261