Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1026-1035 |
| Number of pages | 10 |
| Journal | Materials Science and Engineering: C |
| Volume | 69 |
| Early online date | 2 Aug 2016 |
| DOIs | |
| Publication status | Published - 1 Dec 2016 |
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Synthesis and evaluation of temperature- and glucose-sensitive nanoparticles based on phenylboronic acid and N-vinylcaprolactam for insulin delivery. / Wu, Jun-zi; Bremner, David H.; Li, He-yu; Sun, Xiao-zhu; Zhu, Li-Min.
In: Materials Science and Engineering: C, Vol. 69, 01.12.2016, p. 1026-1035.Research output: Contribution to journal › Article
TY - JOUR
T1 - Synthesis and evaluation of temperature- and glucose-sensitive nanoparticles based on phenylboronic acid and N-vinylcaprolactam for insulin delivery
AU - Wu, Jun-zi
AU - Bremner, David H.
AU - Li, He-yu
AU - Sun, Xiao-zhu
AU - Zhu, Li-Min
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Poly N-vinylcaprolactam-co-acrylamidophenylboronic acid p(NVCL-co-AAPBA) was prepared from N-vinylcaprolactam (NVCL) and 3-acrylamidophenylboronic acid (AAPBA), using 2,2-azobisisobutyronitrile (AIBN) as initiator. The synthesis and structure of the polymer were examined by Fourier Transform infrared spectroscopy (FT-IR) and 1H-NMR. Dynamic light scattering (DLS), lower critical solution temperature (LCST) and transmission electron microscopy (TEM) were utilized to characterize the nanoparticles, CD spectroscopy was used to determine if there were any changes to the conformation of the insulin, and cell and animal toxicity were also investigated. The prepared nanoparticles were found to be monodisperse submicron particles and were glucose- and temperature-sensitive. In addition, the nanoparticles have good insulin-loading characteristics, do not affect the conformation of the insulin and show low-toxicity to cells and animals. These p(NVCL-co-AAPBA) nanoparticles may have some value for insulin or other hypoglycemic protein delivery.
AB - Poly N-vinylcaprolactam-co-acrylamidophenylboronic acid p(NVCL-co-AAPBA) was prepared from N-vinylcaprolactam (NVCL) and 3-acrylamidophenylboronic acid (AAPBA), using 2,2-azobisisobutyronitrile (AIBN) as initiator. The synthesis and structure of the polymer were examined by Fourier Transform infrared spectroscopy (FT-IR) and 1H-NMR. Dynamic light scattering (DLS), lower critical solution temperature (LCST) and transmission electron microscopy (TEM) were utilized to characterize the nanoparticles, CD spectroscopy was used to determine if there were any changes to the conformation of the insulin, and cell and animal toxicity were also investigated. The prepared nanoparticles were found to be monodisperse submicron particles and were glucose- and temperature-sensitive. In addition, the nanoparticles have good insulin-loading characteristics, do not affect the conformation of the insulin and show low-toxicity to cells and animals. These p(NVCL-co-AAPBA) nanoparticles may have some value for insulin or other hypoglycemic protein delivery.
U2 - 10.1016/j.msec.2016.07.078
DO - 10.1016/j.msec.2016.07.078
M3 - Article
VL - 69
SP - 1026
EP - 1035
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -