Synthesis and structural characterization of new ruthenium(II) complexes and investigation of their antiproliferative and metastatic effect against human lung cancer (A549) cells

P. Kalaivani, R. Prabhakaran, Poornima Paramasivan, R. Huang, V. Hornebecq, F. Dallemer, V. Vijaya Padma, K. Natarajan

Research output: Contribution to journalArticle

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Abstract

Reaction of 2-hydroxy-1-naphthaldehyde-4(N)-ethylthiosemicarbazone [H2-(Nap-etsc)] with [RuHCl(CO)(PPh3)3] conferred two entities, namely [Ru(H-Nap-etsc)Cl(CO)(PPh3)2] (1) and [Ru(Nap-etsc)(CO)(PPh3)2]·Cl (2). The new complexes were characterized using various spectroanalytical and X-ray crystallographic techniques. In this reaction the dual coordination behaviour (thiolate/thione) of the ligand was observed. It acted as NS bidentate monobasic and ONS tridentate monobasic in entities 1 and 2 respectively. The binding ability of complexes (1 and 2) to calf thymus DNA (CT DNA)/BSA has been explored by absorption and emission titration methods. The cytotoxic nature of the complexes was evaluated on human lung cancer cells, A549. Complexes induced apoptotic cell death via ROS hypergeneration and mitochondrial membrane damage. In addition these newly synthesized ruthenium complexes inhibited A549 cell migration, as evidenced by wound healing assay. The activity of the complexes was found to be very high by comparing with cisplatin, a conventional standard. The time dependent release of the complexes from porous system was investigated by taking mesoporous silica as the host material. It was shown that the main portion of the embedded complexes was released after 20 h and reached a maximum after 96 h.
Original languageEnglish
Pages (from-to)20363-20378
Number of pages16
JournalRSC Advances
Issue number43
Early online date16 Aug 2013
DOIs
Publication statusPublished - 21 Nov 2013
Externally publishedYes

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Thymus
Ruthenium
Cell death
Carbon Monoxide
Titration
Assays
DNA
Ligands
Silica
Cells
Membranes
X rays
Thiones
Silicon Dioxide
Cisplatin

Cite this

Kalaivani, P. ; Prabhakaran, R. ; Paramasivan, Poornima ; Huang, R. ; Hornebecq, V. ; Dallemer, F. ; Vijaya Padma, V. ; Natarajan, K. / Synthesis and structural characterization of new ruthenium(II) complexes and investigation of their antiproliferative and metastatic effect against human lung cancer (A549) cells. In: RSC Advances. 2013 ; No. 43. pp. 20363-20378 .
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abstract = "Reaction of 2-hydroxy-1-naphthaldehyde-4(N)-ethylthiosemicarbazone [H2-(Nap-etsc)] with [RuHCl(CO)(PPh3)3] conferred two entities, namely [Ru(H-Nap-etsc)Cl(CO)(PPh3)2] (1) and [Ru(Nap-etsc)(CO)(PPh3)2]·Cl (2). The new complexes were characterized using various spectroanalytical and X-ray crystallographic techniques. In this reaction the dual coordination behaviour (thiolate/thione) of the ligand was observed. It acted as NS bidentate monobasic and ONS tridentate monobasic in entities 1 and 2 respectively. The binding ability of complexes (1 and 2) to calf thymus DNA (CT DNA)/BSA has been explored by absorption and emission titration methods. The cytotoxic nature of the complexes was evaluated on human lung cancer cells, A549. Complexes induced apoptotic cell death via ROS hypergeneration and mitochondrial membrane damage. In addition these newly synthesized ruthenium complexes inhibited A549 cell migration, as evidenced by wound healing assay. The activity of the complexes was found to be very high by comparing with cisplatin, a conventional standard. The time dependent release of the complexes from porous system was investigated by taking mesoporous silica as the host material. It was shown that the main portion of the embedded complexes was released after 20 h and reached a maximum after 96 h.",
author = "P. Kalaivani and R. Prabhakaran and Poornima Paramasivan and R. Huang and V. Hornebecq and F. Dallemer and {Vijaya Padma}, V. and K. Natarajan",
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Synthesis and structural characterization of new ruthenium(II) complexes and investigation of their antiproliferative and metastatic effect against human lung cancer (A549) cells. / Kalaivani, P.; Prabhakaran, R.; Paramasivan, Poornima; Huang, R.; Hornebecq, V.; Dallemer, F.; Vijaya Padma, V.; Natarajan, K.

In: RSC Advances, No. 43, 21.11.2013, p. 20363-20378 .

Research output: Contribution to journalArticle

TY - JOUR

T1 - Synthesis and structural characterization of new ruthenium(II) complexes and investigation of their antiproliferative and metastatic effect against human lung cancer (A549) cells

AU - Kalaivani, P.

AU - Prabhakaran, R.

AU - Paramasivan, Poornima

AU - Huang, R.

AU - Hornebecq, V.

AU - Dallemer, F.

AU - Vijaya Padma, V.

AU - Natarajan, K.

PY - 2013/11/21

Y1 - 2013/11/21

N2 - Reaction of 2-hydroxy-1-naphthaldehyde-4(N)-ethylthiosemicarbazone [H2-(Nap-etsc)] with [RuHCl(CO)(PPh3)3] conferred two entities, namely [Ru(H-Nap-etsc)Cl(CO)(PPh3)2] (1) and [Ru(Nap-etsc)(CO)(PPh3)2]·Cl (2). The new complexes were characterized using various spectroanalytical and X-ray crystallographic techniques. In this reaction the dual coordination behaviour (thiolate/thione) of the ligand was observed. It acted as NS bidentate monobasic and ONS tridentate monobasic in entities 1 and 2 respectively. The binding ability of complexes (1 and 2) to calf thymus DNA (CT DNA)/BSA has been explored by absorption and emission titration methods. The cytotoxic nature of the complexes was evaluated on human lung cancer cells, A549. Complexes induced apoptotic cell death via ROS hypergeneration and mitochondrial membrane damage. In addition these newly synthesized ruthenium complexes inhibited A549 cell migration, as evidenced by wound healing assay. The activity of the complexes was found to be very high by comparing with cisplatin, a conventional standard. The time dependent release of the complexes from porous system was investigated by taking mesoporous silica as the host material. It was shown that the main portion of the embedded complexes was released after 20 h and reached a maximum after 96 h.

AB - Reaction of 2-hydroxy-1-naphthaldehyde-4(N)-ethylthiosemicarbazone [H2-(Nap-etsc)] with [RuHCl(CO)(PPh3)3] conferred two entities, namely [Ru(H-Nap-etsc)Cl(CO)(PPh3)2] (1) and [Ru(Nap-etsc)(CO)(PPh3)2]·Cl (2). The new complexes were characterized using various spectroanalytical and X-ray crystallographic techniques. In this reaction the dual coordination behaviour (thiolate/thione) of the ligand was observed. It acted as NS bidentate monobasic and ONS tridentate monobasic in entities 1 and 2 respectively. The binding ability of complexes (1 and 2) to calf thymus DNA (CT DNA)/BSA has been explored by absorption and emission titration methods. The cytotoxic nature of the complexes was evaluated on human lung cancer cells, A549. Complexes induced apoptotic cell death via ROS hypergeneration and mitochondrial membrane damage. In addition these newly synthesized ruthenium complexes inhibited A549 cell migration, as evidenced by wound healing assay. The activity of the complexes was found to be very high by comparing with cisplatin, a conventional standard. The time dependent release of the complexes from porous system was investigated by taking mesoporous silica as the host material. It was shown that the main portion of the embedded complexes was released after 20 h and reached a maximum after 96 h.

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DO - 10.1039/C3RA43335H

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JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

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ER -