Synthesis, DNA/protein binding and in vitro cytotoxic studies of new palladium metallothiosemicarbazones

R. Prabhakaran; P. Kalaivani; P. Poornima; F. Dallemer; R. Huang; V. Vijaya Padma; K. Natarajan

doi:10.1016/j.bmc.2013.08.005

Synthesis, DNA/protein binding and in vitro cytotoxic studies of new palladium metallothiosemicarbazones

R. Prabhakaran^*, P. Kalaivani, P. Poornima, F. Dallemer, R. Huang, V. Vijaya Padma, K. Natarajan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

A series of four new thiosemicarbazone complexes of palladium have been synthesized, characterized and evaluated for their DNA/protein binding with CT-DNA and BSA, respectively. The new complexes bound to CT-DNA by intercalation mode and in protein binding studies, the complexes bound to BSA binding mechanism was found as static quenching. Among them the complex 4 had a strong binding affinity with BSA. In addition, in vitro cytotoxic studies were carried out on lung cancer (A549) and liver cancer (HepG2) cell lines and found that the complexes exhibited better activity than their parent thiosemicarbazone analogues. The complex 3 exhibited better activity than other complexes and this fact supported by the increased accumulation of the complexes in to the cancer cells which are evident from inter cellular uptake studies. (C) 2013 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	6742-6752
Number of pages	11
Journal	Bioorganic and Medicinal Chemistry
Volume	21
Issue number	21
Early online date	13 Aug 2013
DOIs	https://doi.org/10.1016/j.bmc.2013.08.005
Publication status	Published - 1 Nov 2013
Externally published	Yes

Keywords

Thiosemicarbazones
Palladium(II) complexes
CT-DNA binding
BSA binding
Cytotoxicity
Cellular uptake

Access to Document

10.1016/j.bmc.2013.08.005

Cite this

@article{7553ccff1b6046e9bbf0d8b1579f93bb,

title = "Synthesis, DNA/protein binding and in vitro cytotoxic studies of new palladium metallothiosemicarbazones",

abstract = "A series of four new thiosemicarbazone complexes of palladium have been synthesized, characterized and evaluated for their DNA/protein binding with CT-DNA and BSA, respectively. The new complexes bound to CT-DNA by intercalation mode and in protein binding studies, the complexes bound to BSA binding mechanism was found as static quenching. Among them the complex 4 had a strong binding affinity with BSA. In addition, in vitro cytotoxic studies were carried out on lung cancer (A549) and liver cancer (HepG2) cell lines and found that the complexes exhibited better activity than their parent thiosemicarbazone analogues. The complex 3 exhibited better activity than other complexes and this fact supported by the increased accumulation of the complexes in to the cancer cells which are evident from inter cellular uptake studies. (C) 2013 Elsevier Ltd. All rights reserved.",

author = "R. Prabhakaran and P. Kalaivani and P. Poornima and F. Dallemer and R. Huang and Padma, {V. Vijaya} and K. Natarajan",

year = "2013",

month = nov,

day = "1",

doi = "10.1016/j.bmc.2013.08.005",

language = "English",

volume = "21",

pages = "6742--6752",

journal = "Bioorganic and Medicinal Chemistry",

issn = "0968-0896",

publisher = "PERGAMON-ELSEVIER SCIENCE LTD",

number = "21",

}

TY - JOUR

T1 - Synthesis, DNA/protein binding and in vitro cytotoxic studies of new palladium metallothiosemicarbazones

AU - Prabhakaran, R.

AU - Kalaivani, P.

AU - Poornima, P.

AU - Dallemer, F.

AU - Huang, R.

AU - Padma, V. Vijaya

AU - Natarajan, K.

PY - 2013/11/1

Y1 - 2013/11/1

N2 - A series of four new thiosemicarbazone complexes of palladium have been synthesized, characterized and evaluated for their DNA/protein binding with CT-DNA and BSA, respectively. The new complexes bound to CT-DNA by intercalation mode and in protein binding studies, the complexes bound to BSA binding mechanism was found as static quenching. Among them the complex 4 had a strong binding affinity with BSA. In addition, in vitro cytotoxic studies were carried out on lung cancer (A549) and liver cancer (HepG2) cell lines and found that the complexes exhibited better activity than their parent thiosemicarbazone analogues. The complex 3 exhibited better activity than other complexes and this fact supported by the increased accumulation of the complexes in to the cancer cells which are evident from inter cellular uptake studies. (C) 2013 Elsevier Ltd. All rights reserved.

AB - A series of four new thiosemicarbazone complexes of palladium have been synthesized, characterized and evaluated for their DNA/protein binding with CT-DNA and BSA, respectively. The new complexes bound to CT-DNA by intercalation mode and in protein binding studies, the complexes bound to BSA binding mechanism was found as static quenching. Among them the complex 4 had a strong binding affinity with BSA. In addition, in vitro cytotoxic studies were carried out on lung cancer (A549) and liver cancer (HepG2) cell lines and found that the complexes exhibited better activity than their parent thiosemicarbazone analogues. The complex 3 exhibited better activity than other complexes and this fact supported by the increased accumulation of the complexes in to the cancer cells which are evident from inter cellular uptake studies. (C) 2013 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.bmc.2013.08.005

DO - 10.1016/j.bmc.2013.08.005

M3 - Article

VL - 21

SP - 6742

EP - 6752

JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

SN - 0968-0896

IS - 21

ER -