Synthesis, structure, DNA/protein binding and in vitro cytotoxicity of new ruthenium metallates

P. Kalaivani*, R. Prabhakaran, E. Vaishnavi, T. Rueffer, H. Lang, P. Poornima, R. Renganathan, V. Vijaya Padma, K. Natarajan

*Corresponding author for this work

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The reaction of [RuHCl(CO)(PPh3)(3)] with an equimolar amount of salicylaldehyde-4(N)-methylthiosemicarbazone [H-2-(Sal-mtsc)] resulted in two entities, namely [Ru(H-Sal-mtsc)Cl(CO) (PPh3)(2)] (1) and [Ru(Salmtsc)(CO)(PPh3)(2)] (2) from a single tub. The new complexes were characterized by various spectro (IR, absorption and NMR), analytical and single crystal X-ray diffraction studies. From the crystallographic studies, it is confirmed that in complex 1, the ligand coordinated through the thiolate sulfur and the deprotonated hydrazinic nitrogen N(2), resulting in the formation of an unusual strained four membered chelate ring. The third potential donor, phenolic oxygen, remained uncoordinated. In complex 2, the ligand coordinated as an ONS chelate with the formation of more common five and six membered chelate rings. Complexes 1 and 2 have been tested for their DNA/protein binding properties by taking CT-DNA/lysozyme as models. From the protein binding studies, the alterations in the secondary structure of lysozyme by the ruthenium(II) complexes (1 and 2) were confirmed with synchronous and three-dimensional fluorescence spectroscopic studies. The in vitro cytotoxicity of the newly-synthesized complexes was carried out in two different human tumour cell lines, A549 and HepG2. The cytotoxicity studies showed that complex 2 exhibited higher activity than 1.

Original languageEnglish
Pages (from-to)311-324
Number of pages14
JournalInorganic Chemistry Frontiers
Volume1
Issue number4
Early online date24 Jan 2014
DOIs
Publication statusPublished - 1 Apr 2014
Externally publishedYes

Cite this

Kalaivani, P., Prabhakaran, R., Vaishnavi, E., Rueffer, T., Lang, H., Poornima, P., ... Natarajan, K. (2014). Synthesis, structure, DNA/protein binding and in vitro cytotoxicity of new ruthenium metallates. Inorganic Chemistry Frontiers, 1(4), 311-324. https://doi.org/10.1039/c3qi00070b
Kalaivani, P. ; Prabhakaran, R. ; Vaishnavi, E. ; Rueffer, T. ; Lang, H. ; Poornima, P. ; Renganathan, R. ; Padma, V. Vijaya ; Natarajan, K. / Synthesis, structure, DNA/protein binding and in vitro cytotoxicity of new ruthenium metallates. In: Inorganic Chemistry Frontiers. 2014 ; Vol. 1, No. 4. pp. 311-324.
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Kalaivani, P, Prabhakaran, R, Vaishnavi, E, Rueffer, T, Lang, H, Poornima, P, Renganathan, R, Padma, VV & Natarajan, K 2014, 'Synthesis, structure, DNA/protein binding and in vitro cytotoxicity of new ruthenium metallates', Inorganic Chemistry Frontiers, vol. 1, no. 4, pp. 311-324. https://doi.org/10.1039/c3qi00070b

Synthesis, structure, DNA/protein binding and in vitro cytotoxicity of new ruthenium metallates. / Kalaivani, P.; Prabhakaran, R.; Vaishnavi, E.; Rueffer, T.; Lang, H.; Poornima, P.; Renganathan, R.; Padma, V. Vijaya; Natarajan, K.

In: Inorganic Chemistry Frontiers, Vol. 1, No. 4, 01.04.2014, p. 311-324.

Research output: Contribution to journalArticle

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T1 - Synthesis, structure, DNA/protein binding and in vitro cytotoxicity of new ruthenium metallates

AU - Kalaivani, P.

AU - Prabhakaran, R.

AU - Vaishnavi, E.

AU - Rueffer, T.

AU - Lang, H.

AU - Poornima, P.

AU - Renganathan, R.

AU - Padma, V. Vijaya

AU - Natarajan, K.

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Y1 - 2014/4/1

N2 - The reaction of [RuHCl(CO)(PPh3)(3)] with an equimolar amount of salicylaldehyde-4(N)-methylthiosemicarbazone [H-2-(Sal-mtsc)] resulted in two entities, namely [Ru(H-Sal-mtsc)Cl(CO) (PPh3)(2)] (1) and [Ru(Salmtsc)(CO)(PPh3)(2)] (2) from a single tub. The new complexes were characterized by various spectro (IR, absorption and NMR), analytical and single crystal X-ray diffraction studies. From the crystallographic studies, it is confirmed that in complex 1, the ligand coordinated through the thiolate sulfur and the deprotonated hydrazinic nitrogen N(2), resulting in the formation of an unusual strained four membered chelate ring. The third potential donor, phenolic oxygen, remained uncoordinated. In complex 2, the ligand coordinated as an ONS chelate with the formation of more common five and six membered chelate rings. Complexes 1 and 2 have been tested for their DNA/protein binding properties by taking CT-DNA/lysozyme as models. From the protein binding studies, the alterations in the secondary structure of lysozyme by the ruthenium(II) complexes (1 and 2) were confirmed with synchronous and three-dimensional fluorescence spectroscopic studies. The in vitro cytotoxicity of the newly-synthesized complexes was carried out in two different human tumour cell lines, A549 and HepG2. The cytotoxicity studies showed that complex 2 exhibited higher activity than 1.

AB - The reaction of [RuHCl(CO)(PPh3)(3)] with an equimolar amount of salicylaldehyde-4(N)-methylthiosemicarbazone [H-2-(Sal-mtsc)] resulted in two entities, namely [Ru(H-Sal-mtsc)Cl(CO) (PPh3)(2)] (1) and [Ru(Salmtsc)(CO)(PPh3)(2)] (2) from a single tub. The new complexes were characterized by various spectro (IR, absorption and NMR), analytical and single crystal X-ray diffraction studies. From the crystallographic studies, it is confirmed that in complex 1, the ligand coordinated through the thiolate sulfur and the deprotonated hydrazinic nitrogen N(2), resulting in the formation of an unusual strained four membered chelate ring. The third potential donor, phenolic oxygen, remained uncoordinated. In complex 2, the ligand coordinated as an ONS chelate with the formation of more common five and six membered chelate rings. Complexes 1 and 2 have been tested for their DNA/protein binding properties by taking CT-DNA/lysozyme as models. From the protein binding studies, the alterations in the secondary structure of lysozyme by the ruthenium(II) complexes (1 and 2) were confirmed with synchronous and three-dimensional fluorescence spectroscopic studies. The in vitro cytotoxicity of the newly-synthesized complexes was carried out in two different human tumour cell lines, A549 and HepG2. The cytotoxicity studies showed that complex 2 exhibited higher activity than 1.

U2 - 10.1039/c3qi00070b

DO - 10.1039/c3qi00070b

M3 - Article

VL - 1

SP - 311

EP - 324

JO - Inorganic Chemistry Frontiers

JF - Inorganic Chemistry Frontiers

SN - 2052-1545

IS - 4

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Kalaivani P, Prabhakaran R, Vaishnavi E, Rueffer T, Lang H, Poornima P et al. Synthesis, structure, DNA/protein binding and in vitro cytotoxicity of new ruthenium metallates. Inorganic Chemistry Frontiers. 2014 Apr 1;1(4):311-324. https://doi.org/10.1039/c3qi00070b