The basic chemistry of exercise-induced DNA oxidation

oxidative damage, redox signalling and their interplay

James N. Cobley, Nikos V. Margaritelis, James P. Morton, Graeme L. Close, Michalis G. Nikolaidis, John K. Malone

Research output: Contribution to journalArticle

13 Citations (Scopus)
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Abstract

Acute exercise increases reactive oxygen and nitrogen species generation. This phenomenon is associated with two major outcomes: (1) redox signalling and (2) macromolecule damage. Mechanistic knowledge of how exercise-induced redox signalling and macromolecule damage are interlinked is limited. This review focuses on the interplay between exercise-induced redox signalling and DNA damage, using hydroxyl radical (·OH) and hydrogen peroxide (H2O2) as exemplars. It is postulated that the biological fate of H2O2 links the two processes and thus represents a bifurcation point between redox signalling and damage. Indeed, H2O2 can participate in two electron signalling reactions but its diffusion and chemical properties permit DNA oxidation following reaction with transition metals and ·OH generation. It is also considered that the sensing of DNA oxidation by repair proteins constitutes a non-canonical redox signalling mechanism. Further layers of interaction are provided by the redox regulation of DNA repair proteins and their capacity to modulate intracellular H2O2 levels. Overall, exercise-induced redox signalling and DNA damage may be interlinked to a greater extent than was previously thought but this requires further investigation.
Original languageEnglish
Article number182
Number of pages8
JournalFrontiers in Physiology
Volume6
DOIs
Publication statusPublished - 17 Jun 2015

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Oxidation-Reduction
DNA
DNA Repair
DNA Damage
Reactive Nitrogen Species
Hydroxyl Radical
Hydrogen Peroxide
Reactive Oxygen Species
Proteins
Metals
Electrons
Exercise

Cite this

Cobley, James N. ; Margaritelis, Nikos V. ; Morton, James P. ; Close, Graeme L. ; Nikolaidis, Michalis G. ; Malone, John K. / The basic chemistry of exercise-induced DNA oxidation : oxidative damage, redox signalling and their interplay. In: Frontiers in Physiology. 2015 ; Vol. 6.
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The basic chemistry of exercise-induced DNA oxidation : oxidative damage, redox signalling and their interplay. / Cobley, James N.; Margaritelis, Nikos V.; Morton, James P.; Close, Graeme L.; Nikolaidis, Michalis G.; Malone, John K.

In: Frontiers in Physiology, Vol. 6, 182, 17.06.2015.

Research output: Contribution to journalArticle

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AU - Cobley, James N.

AU - Margaritelis, Nikos V.

AU - Morton, James P.

AU - Close, Graeme L.

AU - Nikolaidis, Michalis G.

AU - Malone, John K.

PY - 2015/6/17

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AB - Acute exercise increases reactive oxygen and nitrogen species generation. This phenomenon is associated with two major outcomes: (1) redox signalling and (2) macromolecule damage. Mechanistic knowledge of how exercise-induced redox signalling and macromolecule damage are interlinked is limited. This review focuses on the interplay between exercise-induced redox signalling and DNA damage, using hydroxyl radical (·OH) and hydrogen peroxide (H2O2) as exemplars. It is postulated that the biological fate of H2O2 links the two processes and thus represents a bifurcation point between redox signalling and damage. Indeed, H2O2 can participate in two electron signalling reactions but its diffusion and chemical properties permit DNA oxidation following reaction with transition metals and ·OH generation. It is also considered that the sensing of DNA oxidation by repair proteins constitutes a non-canonical redox signalling mechanism. Further layers of interaction are provided by the redox regulation of DNA repair proteins and their capacity to modulate intracellular H2O2 levels. Overall, exercise-induced redox signalling and DNA damage may be interlinked to a greater extent than was previously thought but this requires further investigation.

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