The bioremediation of copper from distillery wastewater using indigenous microbes

Preeti Parikh, Graeme M. Walker, Phillip J. Collier, Douglas Murray

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Erosion of copper from the internal surface of the distillery vessels has been a major environmental concern mainly due to discharge of high concentrations of copper through drainage systems. The present study was designed to characterize the metal uptake and biosorption behaviour of indigenous microbes present and isolated from distillery environments. Bacillus licheniformis was isolated from distillery sump waters and identified using the API 50CH/B system. The minimum inhibitory concentration of copper against this organism ranged between 40-50mg/L. Biosorption capacities of B.licheniformis and a control laboratory strain B. subtilis 8045 for copper was determined using batch and continuous column experiments. Biosorption capacities for both strains was carried out at pH 5 and at copper concentrations of 10,50,70,90 and 200 mg/L. Immobilized bacterial studies of copper bioremediation were carried out with test and control strains using 2% calcium alginate beads to immobilize cells. Several parameters were optimized such as pH, bead size, and copper concentrations at which maximum biosorption can take place. Column bioreactor studies were done at an optimized pH and most efficient bead size and the effluent was collected at a flow rate of 1ml/min at every hour. Copper retention time in the immobilized column showed 90% removal bringing the copper levels down to below threshold levels in the first 10 hours of the column flow. The metal recovery was performed using 0.1M HCl and low pH solutions. The results of this study indicate the potential of bacterial copper bioremediation systems for distillery wastewaters.
    Original languageEnglish
    Title of host publicationDistilled spirits
    Subtitle of host publicationnew horizons: energy, environment and enlightenment
    EditorsG.M. Walker, P.S. Hughes
    Place of PublicationNottingham
    PublisherNottingham University Press
    Chapter26
    Pages179-196
    Number of pages18
    ISBN (Print)9781907284458
    Publication statusPublished - 2010
    EventWorldwide Distilled Spirits Conference - Edinburgh, United Kingdom
    Duration: 1 Sep 2008 → …
    Conference number: 3

    Conference

    ConferenceWorldwide Distilled Spirits Conference
    Abbreviated titleWDSC
    CountryUnited Kingdom
    CityEdinburgh
    Period1/09/08 → …

    Fingerprint

    bioremediation
    copper
    wastewater
    alginate
    metal
    bioreactor
    vessel
    calcium
    effluent
    erosion

    Cite this

    Parikh, P., Walker, G. M., Collier, P. J., & Murray, D. (2010). The bioremediation of copper from distillery wastewater using indigenous microbes. In G. M. Walker, & P. S. Hughes (Eds.), Distilled spirits: new horizons: energy, environment and enlightenment (pp. 179-196). Nottingham: Nottingham University Press.
    Parikh, Preeti ; Walker, Graeme M. ; Collier, Phillip J. ; Murray, Douglas. / The bioremediation of copper from distillery wastewater using indigenous microbes. Distilled spirits: new horizons: energy, environment and enlightenment. editor / G.M. Walker ; P.S. Hughes. Nottingham : Nottingham University Press, 2010. pp. 179-196
    @inproceedings{b87614d4c47d4355a23f7bb9fbf1e635,
    title = "The bioremediation of copper from distillery wastewater using indigenous microbes",
    abstract = "Erosion of copper from the internal surface of the distillery vessels has been a major environmental concern mainly due to discharge of high concentrations of copper through drainage systems. The present study was designed to characterize the metal uptake and biosorption behaviour of indigenous microbes present and isolated from distillery environments. Bacillus licheniformis was isolated from distillery sump waters and identified using the API 50CH/B system. The minimum inhibitory concentration of copper against this organism ranged between 40-50mg/L. Biosorption capacities of B.licheniformis and a control laboratory strain B. subtilis 8045 for copper was determined using batch and continuous column experiments. Biosorption capacities for both strains was carried out at pH 5 and at copper concentrations of 10,50,70,90 and 200 mg/L. Immobilized bacterial studies of copper bioremediation were carried out with test and control strains using 2{\%} calcium alginate beads to immobilize cells. Several parameters were optimized such as pH, bead size, and copper concentrations at which maximum biosorption can take place. Column bioreactor studies were done at an optimized pH and most efficient bead size and the effluent was collected at a flow rate of 1ml/min at every hour. Copper retention time in the immobilized column showed 90{\%} removal bringing the copper levels down to below threshold levels in the first 10 hours of the column flow. The metal recovery was performed using 0.1M HCl and low pH solutions. The results of this study indicate the potential of bacterial copper bioremediation systems for distillery wastewaters.",
    author = "Preeti Parikh and Walker, {Graeme M.} and Collier, {Phillip J.} and Douglas Murray",
    year = "2010",
    language = "English",
    isbn = "9781907284458",
    pages = "179--196",
    editor = "Walker, {G.M. } and P.S. Hughes",
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    publisher = "Nottingham University Press",
    address = "United Kingdom",

    }

    Parikh, P, Walker, GM, Collier, PJ & Murray, D 2010, The bioremediation of copper from distillery wastewater using indigenous microbes. in GM Walker & PS Hughes (eds), Distilled spirits: new horizons: energy, environment and enlightenment. Nottingham University Press, Nottingham, pp. 179-196, Worldwide Distilled Spirits Conference, Edinburgh, United Kingdom, 1/09/08.

    The bioremediation of copper from distillery wastewater using indigenous microbes. / Parikh, Preeti; Walker, Graeme M.; Collier, Phillip J.; Murray, Douglas.

    Distilled spirits: new horizons: energy, environment and enlightenment. ed. / G.M. Walker; P.S. Hughes. Nottingham : Nottingham University Press, 2010. p. 179-196.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    TY - GEN

    T1 - The bioremediation of copper from distillery wastewater using indigenous microbes

    AU - Parikh, Preeti

    AU - Walker, Graeme M.

    AU - Collier, Phillip J.

    AU - Murray, Douglas

    PY - 2010

    Y1 - 2010

    N2 - Erosion of copper from the internal surface of the distillery vessels has been a major environmental concern mainly due to discharge of high concentrations of copper through drainage systems. The present study was designed to characterize the metal uptake and biosorption behaviour of indigenous microbes present and isolated from distillery environments. Bacillus licheniformis was isolated from distillery sump waters and identified using the API 50CH/B system. The minimum inhibitory concentration of copper against this organism ranged between 40-50mg/L. Biosorption capacities of B.licheniformis and a control laboratory strain B. subtilis 8045 for copper was determined using batch and continuous column experiments. Biosorption capacities for both strains was carried out at pH 5 and at copper concentrations of 10,50,70,90 and 200 mg/L. Immobilized bacterial studies of copper bioremediation were carried out with test and control strains using 2% calcium alginate beads to immobilize cells. Several parameters were optimized such as pH, bead size, and copper concentrations at which maximum biosorption can take place. Column bioreactor studies were done at an optimized pH and most efficient bead size and the effluent was collected at a flow rate of 1ml/min at every hour. Copper retention time in the immobilized column showed 90% removal bringing the copper levels down to below threshold levels in the first 10 hours of the column flow. The metal recovery was performed using 0.1M HCl and low pH solutions. The results of this study indicate the potential of bacterial copper bioremediation systems for distillery wastewaters.

    AB - Erosion of copper from the internal surface of the distillery vessels has been a major environmental concern mainly due to discharge of high concentrations of copper through drainage systems. The present study was designed to characterize the metal uptake and biosorption behaviour of indigenous microbes present and isolated from distillery environments. Bacillus licheniformis was isolated from distillery sump waters and identified using the API 50CH/B system. The minimum inhibitory concentration of copper against this organism ranged between 40-50mg/L. Biosorption capacities of B.licheniformis and a control laboratory strain B. subtilis 8045 for copper was determined using batch and continuous column experiments. Biosorption capacities for both strains was carried out at pH 5 and at copper concentrations of 10,50,70,90 and 200 mg/L. Immobilized bacterial studies of copper bioremediation were carried out with test and control strains using 2% calcium alginate beads to immobilize cells. Several parameters were optimized such as pH, bead size, and copper concentrations at which maximum biosorption can take place. Column bioreactor studies were done at an optimized pH and most efficient bead size and the effluent was collected at a flow rate of 1ml/min at every hour. Copper retention time in the immobilized column showed 90% removal bringing the copper levels down to below threshold levels in the first 10 hours of the column flow. The metal recovery was performed using 0.1M HCl and low pH solutions. The results of this study indicate the potential of bacterial copper bioremediation systems for distillery wastewaters.

    M3 - Conference contribution

    SN - 9781907284458

    SP - 179

    EP - 196

    BT - Distilled spirits

    A2 - Walker, G.M.

    A2 - Hughes, P.S.

    PB - Nottingham University Press

    CY - Nottingham

    ER -

    Parikh P, Walker GM, Collier PJ, Murray D. The bioremediation of copper from distillery wastewater using indigenous microbes. In Walker GM, Hughes PS, editors, Distilled spirits: new horizons: energy, environment and enlightenment. Nottingham: Nottingham University Press. 2010. p. 179-196