The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

Victor V. Revin, Natalia V. Gromova, Elvira S. Revina, Igor P. Grunyushkin, Alexander Yu. Tychkov, Anastasia Yu. Samonova, Anastasia N. Kukina, Alexander A. Moskovkin, Jean Christophe Bourdon, Nikolai Zhelev

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    Abstract

    The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.
    Original languageEnglish
    Pages (from-to)1236-1250
    Number of pages15
    JournalBiotechnology and Biotechnological Equipment
    Volume32
    Issue number5
    Early online date8 Jan 2018
    DOIs
    Publication statusPublished - 2018

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    Hyperoxia
    Erythrocytes
    Oxygen
    Erythrocyte Membrane
    Ions
    Phospholipids
    Hemoglobins
    Calcium
    Lipids
    Raman Spectrum Analysis
    Silica Gel
    Lipid Bilayers
    Pathologic Processes
    Glass

    Cite this

    Revin, V. V., Gromova, N. V., Revina, E. S., Grunyushkin, I. P., Tychkov, A. Y., Samonova, A. Y., ... Zhelev, N. (2018). The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function. Biotechnology and Biotechnological Equipment, 32(5), 1236-1250. https://doi.org/10.1080/13102818.2017.1414633
    Revin, Victor V. ; Gromova, Natalia V. ; Revina, Elvira S. ; Grunyushkin, Igor P. ; Tychkov, Alexander Yu. ; Samonova, Anastasia Yu. ; Kukina, Anastasia N. ; Moskovkin, Alexander A. ; Bourdon, Jean Christophe ; Zhelev, Nikolai. / The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function. In: Biotechnology and Biotechnological Equipment. 2018 ; Vol. 32, No. 5. pp. 1236-1250.
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    abstract = "The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.",
    author = "Revin, {Victor V.} and Gromova, {Natalia V.} and Revina, {Elvira S.} and Grunyushkin, {Igor P.} and Tychkov, {Alexander Yu.} and Samonova, {Anastasia Yu.} and Kukina, {Anastasia N.} and Moskovkin, {Alexander A.} and Bourdon, {Jean Christophe} and Nikolai Zhelev",
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    Revin, VV, Gromova, NV, Revina, ES, Grunyushkin, IP, Tychkov, AY, Samonova, AY, Kukina, AN, Moskovkin, AA, Bourdon, JC & Zhelev, N 2018, 'The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function', Biotechnology and Biotechnological Equipment, vol. 32, no. 5, pp. 1236-1250. https://doi.org/10.1080/13102818.2017.1414633

    The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function. / Revin, Victor V.; Gromova, Natalia V.; Revina, Elvira S.; Grunyushkin, Igor P.; Tychkov, Alexander Yu.; Samonova, Anastasia Yu.; Kukina, Anastasia N.; Moskovkin, Alexander A.; Bourdon, Jean Christophe; Zhelev, Nikolai.

    In: Biotechnology and Biotechnological Equipment, Vol. 32, No. 5, 2018, p. 1236-1250.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The effect of experimental hyperoxia on erythrocytes’ oxygen-transport function

    AU - Revin, Victor V.

    AU - Gromova, Natalia V.

    AU - Revina, Elvira S.

    AU - Grunyushkin, Igor P.

    AU - Tychkov, Alexander Yu.

    AU - Samonova, Anastasia Yu.

    AU - Kukina, Anastasia N.

    AU - Moskovkin, Alexander A.

    AU - Bourdon, Jean Christophe

    AU - Zhelev, Nikolai

    PY - 2018

    Y1 - 2018

    N2 - The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.

    AB - The aim of this study was to investigate the effect of hyperoxia, calcium ions and pH value on the composition of major phospholipids in human erythrocyte membranes and erythrocytes’ oxygen-transport function. To create a model of hyperoxia, we saturated the incubated mixture with oxygen by constant passing of oxygen–air mixture through the incubation medium. To assess the effect of elevated calcium ion concentrations, CaCl2 was added to the incubation medium. An incubation medium with different pH was used to study the effect of various pH values. Lipids were extracted from erythrocytes and chromatographic separation was carried out in a thin layer of silica gel deposited on a glass plate. The thiobarbituric acid (TBA)-active products and the content of diene conjugates (DC) in erythrocytes were determined. The oxygen-binding capacity of haemoglobin was evaluated using Raman spectroscopy. The obtained results indicated that hyperoxia causes deep changes both in the composition and character of bilayer lipids of erythrocyte membranes, which affects the functional characteristics of erythrocytes, primarily the oxygen-transport properties of erythrocyte haemoglobin. It should be noted that a combination of Ca2+ ions and change in the pH value intensify the processes associated with disruption of phospholipids’ composition. The findings indicate that the lipid phase is one of the key elements in the functioning of erythrocytes in norm as well as during development of various pathological processes.

    U2 - 10.1080/13102818.2017.1414633

    DO - 10.1080/13102818.2017.1414633

    M3 - Article

    VL - 32

    SP - 1236

    EP - 1250

    JO - Biotechnology and Biotechnological Equipment

    JF - Biotechnology and Biotechnological Equipment

    SN - 1310-2818

    IS - 5

    ER -