Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1281-1291 |
| Number of pages | 11 |
| Journal | Journal of Applied Microbiology |
| Volume | 108 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2010 |
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The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed by Staphylococcus aureus biofilms. / Haddadin, R. N. S.; Saleh, S.; Al-Adham, I. S. I.; Buultjens, T. E. J.; Collier, Phillip J.
In: Journal of Applied Microbiology, Vol. 108, No. 4, 04.2010, p. 1281-1291.Research output: Contribution to journal › Article
TY - JOUR
T1 - The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed by Staphylococcus aureus biofilms
AU - Haddadin,R. N. S.
AU - Saleh,S.
AU - Al-Adham,I. S. I.
AU - Buultjens,T. E. J.
AU - Collier,Phillip J.
PY - 2010/4
Y1 - 2010/4
N2 - Aims: The effect of subminimal inhibitory concentrations (sub-MICs) of cefalexin, ciprofloxacin and roxithromycin was investigated on some virulence factors [e.g. coagulase, Toxic Shock Syndrome Toxin 1 (TSST-1) and biofilm formation] expressed by Staphylococcus aureus biofilms. Methods and Results: Biofilms were grown with and without the presence of 1 ⁄ 16 MIC of antibiotics on Sorbarod filters. Eluate supernatants were collected, and coagulase and TSST-1 production were evaluated. Coagulase production was reduced in eluates exposed to roxithromycin when compared to control, while TSST-1 production was reduced in biofilms exposed to cefalexin and to a lesser extent, ciprofloxacin. In addition, the ability of Staph. aureus to produce biofilm in microtitre plates in the presence of sub-MIC antibiotics indicated that cefalexin induced biofilm formation at a wide range of sub- MICs. TSST-1 produced from the challenged and control biofilms was purified, and its proliferative activity was studied on single cell suspension of mouse splenocytes using MTS⁄PMS assay. No significant difference in the activity between the treated toxin and the control has been observed. Conclusions: Antibiotics at sub-MIC levels interfere with bacterial biofilm virulence expression depending on the type and concentration of antibiotic used. Significance and Impact of the Study: The establishment of sub-MICs of antibiotics in clinical situations may result in altered virulence states in pathogenic bacteria.
AB - Aims: The effect of subminimal inhibitory concentrations (sub-MICs) of cefalexin, ciprofloxacin and roxithromycin was investigated on some virulence factors [e.g. coagulase, Toxic Shock Syndrome Toxin 1 (TSST-1) and biofilm formation] expressed by Staphylococcus aureus biofilms. Methods and Results: Biofilms were grown with and without the presence of 1 ⁄ 16 MIC of antibiotics on Sorbarod filters. Eluate supernatants were collected, and coagulase and TSST-1 production were evaluated. Coagulase production was reduced in eluates exposed to roxithromycin when compared to control, while TSST-1 production was reduced in biofilms exposed to cefalexin and to a lesser extent, ciprofloxacin. In addition, the ability of Staph. aureus to produce biofilm in microtitre plates in the presence of sub-MIC antibiotics indicated that cefalexin induced biofilm formation at a wide range of sub- MICs. TSST-1 produced from the challenged and control biofilms was purified, and its proliferative activity was studied on single cell suspension of mouse splenocytes using MTS⁄PMS assay. No significant difference in the activity between the treated toxin and the control has been observed. Conclusions: Antibiotics at sub-MIC levels interfere with bacterial biofilm virulence expression depending on the type and concentration of antibiotic used. Significance and Impact of the Study: The establishment of sub-MICs of antibiotics in clinical situations may result in altered virulence states in pathogenic bacteria.
U2 - 10.1111/j.1365-2672.2009.04529.x
DO - 10.1111/j.1365-2672.2009.04529.x
M3 - Article
VL - 108
SP - 1281
EP - 1291
JO - Journal of Applied Microbiology
T2 - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
SN - 1364-5072
IS - 4
ER -