The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei

M. Adilia Lemos, J. A. Teixeira, M. R. M. Domingues, M. Mota, F. M. Gama

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.
Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalEnzyme and Microbial Technology
Volume32
Issue number1
DOIs
Publication statusPublished - 2 Jan 2003

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Cellulose 1,4-beta-Cellobiosidase
Trichoderma
Cellulase
Cellulose
Hydrolysis
Proteolysis
Cellulases
Substrates
Sugars
Enzymes
Experiments

Cite this

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title = "The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei",
abstract = "The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30{\%}. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16{\%} was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.",
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The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei. / Lemos, M. Adilia; Teixeira, J. A.; Domingues, M. R. M.; Mota, M.; Gama, F. M.

In: Enzyme and Microbial Technology, Vol. 32, No. 1, 02.01.2003, p. 35-40.

Research output: Contribution to journalArticle

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AU - Lemos, M. Adilia

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AU - Domingues, M. R. M.

AU - Mota, M.

AU - Gama, F. M.

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AB - The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.

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