Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 35-40 |
| Number of pages | 6 |
| Journal | Enzyme and Microbial Technology |
| Volume | 32 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2 Jan 2003 |
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The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei. / Lemos, M. Adilia; Teixeira, J. A.; Domingues, M. R. M.; Mota, M.; Gama, F. M.
In: Enzyme and Microbial Technology, Vol. 32, No. 1, 02.01.2003, p. 35-40.Research output: Contribution to journal › Article
TY - JOUR
T1 - The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from Trichoderma reesei
AU - Lemos, M. Adilia
AU - Teixeira, J. A.
AU - Domingues, M. R. M.
AU - Mota, M.
AU - Gama, F. M.
PY - 2003/1/2
Y1 - 2003/1/2
N2 - The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.
AB - The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase.
U2 - 10.1016/S0141-0229(02)00235-1
DO - 10.1016/S0141-0229(02)00235-1
M3 - Article
VL - 32
SP - 35
EP - 40
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
IS - 1
ER -