The regulation of cell division in yeast by magnesium

Graeme M. Walker, John H. Duffus

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cells of the fission yeast, Schizosaccharomyces pombe, and the budding yeast, Kluyveromyces fragilis, fail to divide in Mg-free minimal media but can be induced to divide synchronously on restoration of Mg to “exhausted” cultures. Mg-depleted S. pombe, cells appear abnormally long, lack cell plates and possess elongated nuclei. This, together with evidence of a late cell cycle arrest by the ionophore A23187, suggests that specific Mg limitation blocks cells late in the G2 phase of the cell cycle. Moreover, analysis of cell Mg in cultures synchronized by various techniques reveals that there is a fairly steady fall in Mg concentration as cells grow, terminating in a rapid influx of Mg just before division. These results lead to the hypothesis that intracellular Mg concentration is the transducer for size and consequently time, related control of the cell cycle.
Original languageEnglish
Title of host publicationCurrent developments in yeast research
EditorsGraham G. Stewart, Inge Russell
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Chapter87
Pages561-567
Number of pages7
ISBN (Electronic)9780080253824
ISBN (Print)9780080253657, 0080253652
DOIs
Publication statusPublished - 1981
Externally publishedYes
EventFifth International Yeast Symposium - London, Canada
Duration: 20 Jul 198025 Jul 1980

Publication series

NameAdvances in Biotechnology
PublisherPergamon
Volume4

Conference

ConferenceFifth International Yeast Symposium
CountryCanada
CityLondon
Period20/07/8025/07/80

Fingerprint

Cell Division
Magnesium
Yeasts
Schizosaccharomyces
Cell Cycle Checkpoints
Kluyveromyces
Saccharomycetales
G2 Phase
Ionophores
Calcimycin
Transducers
Cell Cycle

Cite this

Walker, G. M., & Duffus, J. H. (1981). The regulation of cell division in yeast by magnesium. In G. G. Stewart, & I. Russell (Eds.), Current developments in yeast research (pp. 561-567). (Advances in Biotechnology; Vol. 4). PERGAMON-ELSEVIER SCIENCE LTD. https://doi.org/10.1016/C2013-0-03316-6
Walker, Graeme M. ; Duffus, John H. / The regulation of cell division in yeast by magnesium. Current developments in yeast research . editor / Graham G. Stewart ; Inge Russell. PERGAMON-ELSEVIER SCIENCE LTD, 1981. pp. 561-567 (Advances in Biotechnology).
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Walker, GM & Duffus, JH 1981, The regulation of cell division in yeast by magnesium. in GG Stewart & I Russell (eds), Current developments in yeast research . Advances in Biotechnology, vol. 4, PERGAMON-ELSEVIER SCIENCE LTD, pp. 561-567, Fifth International Yeast Symposium, London, Canada, 20/07/80. https://doi.org/10.1016/C2013-0-03316-6

The regulation of cell division in yeast by magnesium. / Walker, Graeme M.; Duffus, John H.

Current developments in yeast research . ed. / Graham G. Stewart; Inge Russell. PERGAMON-ELSEVIER SCIENCE LTD, 1981. p. 561-567 (Advances in Biotechnology; Vol. 4).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Walker GM, Duffus JH. The regulation of cell division in yeast by magnesium. In Stewart GG, Russell I, editors, Current developments in yeast research . PERGAMON-ELSEVIER SCIENCE LTD. 1981. p. 561-567. (Advances in Biotechnology). https://doi.org/10.1016/C2013-0-03316-6