Theoretical mechanisms in targeted and random integration of transgene DNA

Research output: Contribution to journalReview article

25 Citations (Scopus)

Abstract

The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.
Original languageEnglish
Pages (from-to)465-485
Number of pages21
JournalReproduction Nutrition Development
Volume41
Issue number6
DOIs
Publication statusPublished - Dec 2001

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gene targeting
transgenes
homologous recombination
DNA
genetic engineering
host range
cells
animals

Cite this

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title = "Theoretical mechanisms in targeted and random integration of transgene DNA",
abstract = "The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.",
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Theoretical mechanisms in targeted and random integration of transgene DNA. / Smith, Kevin R.

In: Reproduction Nutrition Development, Vol. 41, No. 6, 12.2001, p. 465-485.

Research output: Contribution to journalReview article

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AB - The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.

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