Theoretical mechanisms in targeted and random integration of transgene DNA

Kevin R. Smith

doi:10.1051/rnd:2001102

Theoretical mechanisms in targeted and random integration of transgene DNA

Kevin R. Smith

Abertay University

Research output: Contribution to journal › Review article › peer-review

31 Citations (Scopus)

Abstract

The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.

Original language	English
Pages (from-to)	465-485
Number of pages	21
Journal	Reproduction Nutrition Development
Volume	41
Issue number	6
DOIs	https://doi.org/10.1051/rnd:2001102
Publication status	Published - Dec 2001

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abstract = "The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.",

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AB - The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.

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