Theoretical mechanisms in targeted and random integration of transgene DNA

Kevin R. Smith

doi:10.1051/rnd:2001102

Theoretical mechanisms in targeted and random integration of transgene DNA

Kevin R. Smith

Research output: Contribution to journal › Review article › peer-review

36 Citations (Scopus)

Abstract

The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.

Original language	English
Pages (from-to)	465-485
Number of pages	21
Journal	Reproduction Nutrition Development
Volume	41
Issue number	6
DOIs	https://doi.org/10.1051/rnd:2001102
Publication status	Published - Dec 2001

Keywords

Transgenesis
Gene targeting
Gene therapy
Integration
Homologous recombination
Concatenation

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10.1051/rnd:2001102

The prospects for human germline genetic modification: scientific and bioethical considerations
Smith, K. (Author), Dec 2004
Student thesis: Doctoral Thesis

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AB - The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.

U2 - 10.1051/rnd:2001102

DO - 10.1051/rnd:2001102

M3 - Review article

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SP - 465

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JF - Reproduction Nutrition Development

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Theoretical mechanisms in targeted and random integration of transgene DNA

Abstract

Keywords

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Student theses

The prospects for human germline genetic modification: scientific and bioethical considerations

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