Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis

Alexander Rapoport; Anna Rusakova; Galina Khroustalyova; Graeme Walker

doi:10.1016/j.procbio.2014.07.006

Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis

Alexander Rapoport^*, Anna Rusakova, Galina Khroustalyova, Graeme Walker

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95%. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.

Original language	English
Pages (from-to)	1889-1892
Number of pages	4
Journal	Process Biochemistry
Volume	49
Issue number	11
Early online date	19 Jul 2014
DOIs	https://doi.org/10.1016/j.procbio.2014.07.006
Publication status	Published - Nov 2014

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Graeme Walker
- G510765@abertay.ac.uk
- SAS-EFS-Food & Drink - Professor - Zymology
Person: Academic

Cite this

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title = "Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis",

abstract = "We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95%. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.",

author = "Alexander Rapoport and Anna Rusakova and Galina Khroustalyova and Graeme Walker",

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AU - Rapoport, Alexander

AU - Rusakova, Anna

AU - Khroustalyova, Galina

AU - Walker, Graeme

PY - 2014/11

Y1 - 2014/11

N2 - We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95%. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.

AB - We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95%. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.

U2 - 10.1016/j.procbio.2014.07.006

DO - 10.1016/j.procbio.2014.07.006

M3 - Article

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JO - Process Biochemistry

JF - Process Biochemistry

SN - 0032-9592

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ER -