Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis

Alexander Rapoport, Anna Rusakova, Galina Khroustalyova, Graeme M. Walker

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95%. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.
Original languageEnglish
Pages (from-to)1889-1892
Number of pages4
JournalProcess Biochemistry
Volume49
Issue number11
DOIs
Publication statusPublished - Nov 2014

Fingerprint

Yeast
Saccharomyces cerevisiae
Fluid Therapy
Yeasts
Steam
Eukaryotic Cells
Cell Survival
Cell Membrane
Cell membranes
Thermotolerance
Water vapor
Restoration
Growth
Cells
Therapeutics

Cite this

Rapoport, Alexander ; Rusakova, Anna ; Khroustalyova, Galina ; Walker, Graeme M. / Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis. In: Process Biochemistry. 2014 ; Vol. 49, No. 11. pp. 1889-1892.
@article{139f4c1741e743fe816d4dfec0b7209c,
title = "Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis",
abstract = "We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95{\%}. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.",
author = "Alexander Rapoport and Anna Rusakova and Galina Khroustalyova and Walker, {Graeme M.}",
year = "2014",
month = "11",
doi = "10.1016/j.procbio.2014.07.006",
language = "English",
volume = "49",
pages = "1889--1892",
journal = "Process Biochemistry",
issn = "0032-9592",
publisher = "Elsevier BV",
number = "11",

}

Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis. / Rapoport, Alexander; Rusakova, Anna; Khroustalyova, Galina; Walker, Graeme M.

In: Process Biochemistry, Vol. 49, No. 11, 11.2014, p. 1889-1892.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis

AU - Rapoport, Alexander

AU - Rusakova, Anna

AU - Khroustalyova, Galina

AU - Walker, Graeme M.

PY - 2014/11

Y1 - 2014/11

N2 - We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95%. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.

AB - We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95%. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.

U2 - 10.1016/j.procbio.2014.07.006

DO - 10.1016/j.procbio.2014.07.006

M3 - Article

VL - 49

SP - 1889

EP - 1892

JO - Process Biochemistry

JF - Process Biochemistry

SN - 0032-9592

IS - 11

ER -