Thermotolerance in Saccharomyces cerevisiae is linked to resistance to anhydrobiosis

Alexander Rapoport*, Anna Rusakova, Galina Khroustalyova, Graeme M. Walker

*Corresponding author for this work

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    We have demonstrated that a thermotolerant yeast strain (Saccharomyces cerevisiae TS1) is much more resistant to dehydration–rehydration treatments than a mesophilic strain of S. cerevisiae. Yeast resistance to dehydration–rehydration was found to be similar in cells from exponential and stationary growth phases. Under controlled rehydration conditions involving gradual rehydration in water vapour, yeast cell viability was maintained at 90–95%. When S. cerevisiae TS1 cells were pre-grown at 37 °C and then dried, controlled rehydration lead to restoration of plasma membrane integrity, indicating important differences in cell envelope architechture of mesophilic and thermotolerant yeast strains. Comparison of such strains provides new insight into anhydrobiosis in eukaryotic cells.
    Original languageEnglish
    Pages (from-to)1889-1892
    Number of pages4
    JournalProcess Biochemistry
    Volume49
    Issue number11
    Early online date19 Jul 2014
    DOIs
    Publication statusPublished - Nov 2014

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