Thorough investigation of the phenolic profile of reputable Greek honey varieties: varietal discrimination and floral markers identification using liquid chromatography–high-resolution mass spectrometry

Georgios A. Koulis , Aristeidis S. Tsagkaris, Panagiota A. . Katsianou , Panagiotis-Loukas P. Gialouris, Ioannis Martakos , Fotis Stergiou , Alberto Fiore, Eleni I. Panagopoulou, Sofia Karabournioti , Carsten Baessmann, Noud van der Borg, Marilena E. Dasenaki*, Charalampos Proestos, Nikolaos S. Thomaidis *

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Downloads (Pure)

Abstract

Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied to identify botanical origin markers of Greek honey. Blossom honey samples (n = 62) and the unifloral fir (n = 10), oak (n = 24), pine (n = 39) and thyme (n = 34) honeys were analyzed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) system. Several potential authenticity markers were revealed from the application of different metabolomic workflows. In detail, based on quantitative targeted analysis, three blossom honey markers were found, namely, galangin, pinocembrin and chrysin, while gallic acid concentration was found to be significantly higher in oak honey. Using suspect screening workflow, 12 additional bioactive compounds were identified and semi-quantified, achieving comprehensive metabolomic honey characterization. Lastly, by combining non-targeted screening with advanced chemometrics, it was possible to discriminate thyme from blossom honey and develop binary discriminatory models with high predictive power. In conclusion, a holistic approach to assessing the botanical origin of Greek honey is presented, highlighting the complementarity of the three applied metabolomic approaches.
Original languageEnglish
Article number4444
Number of pages17
JournalMolecules
Volume27
Issue number14
Early online date11 Jul 2022
DOIs
Publication statusPublished - 11 Jul 2022

Keywords

  • Honey
  • Phenolic compounds
  • Metabolomics
  • Greek honey
  • Chemometrics
  • Botanical origin
  • Authenticity
  • Discrimination
  • High-resolution mass spectrometry

Fingerprint

Dive into the research topics of 'Thorough investigation of the phenolic profile of reputable Greek honey varieties: varietal discrimination and floral markers identification using liquid chromatography–high-resolution mass spectrometry'. Together they form a unique fingerprint.

Cite this