TY - JOUR
T1 - Thorough investigation of the phenolic profile of reputable Greek honey varieties
T2 - varietal discrimination and floral markers identification using liquid chromatography–high-resolution mass spectrometry
AU - Koulis , Georgios A.
AU - Tsagkaris, Aristeidis S.
AU - . Katsianou , Panagiota A.
AU - Gialouris, Panagiotis-Loukas P.
AU - Martakos , Ioannis
AU - Stergiou , Fotis
AU - Fiore, Alberto
AU - Panagopoulou, Eleni I.
AU - Karabournioti , Sofia
AU - Baessmann, Carsten
AU - van der Borg, Noud
AU - Dasenaki, Marilena E.
AU - Proestos, Charalampos
AU - Thomaidis , Nikolaos S.
N1 - The authors acknowledge support of this work by the project “FoodOmicsGR – National Research Infrastructure for the Comprehensive Characterization of Foods” (MIS 5029057), which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020) and co‐financed by Greece and the European Union (European Regional Devel‐opment Fund).
PY - 2022/7/11
Y1 - 2022/7/11
N2 - Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied to identify botanical origin markers of Greek honey. Blossom honey samples (n = 62) and the unifloral fir (n = 10), oak (n = 24), pine (n = 39) and thyme (n = 34) honeys were analyzed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) system. Several potential authenticity markers were revealed from the application of different metabolomic workflows. In detail, based on quantitative targeted analysis, three blossom honey markers were found, namely, galangin, pinocembrin and chrysin, while gallic acid concentration was found to be significantly higher in oak honey. Using suspect screening workflow, 12 additional bioactive compounds were identified and semi-quantified, achieving comprehensive metabolomic honey characterization. Lastly, by combining non-targeted screening with advanced chemometrics, it was possible to discriminate thyme from blossom honey and develop binary discriminatory models with high predictive power. In conclusion, a holistic approach to assessing the botanical origin of Greek honey is presented, highlighting the complementarity of the three applied metabolomic approaches.
AB - Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied to identify botanical origin markers of Greek honey. Blossom honey samples (n = 62) and the unifloral fir (n = 10), oak (n = 24), pine (n = 39) and thyme (n = 34) honeys were analyzed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) system. Several potential authenticity markers were revealed from the application of different metabolomic workflows. In detail, based on quantitative targeted analysis, three blossom honey markers were found, namely, galangin, pinocembrin and chrysin, while gallic acid concentration was found to be significantly higher in oak honey. Using suspect screening workflow, 12 additional bioactive compounds were identified and semi-quantified, achieving comprehensive metabolomic honey characterization. Lastly, by combining non-targeted screening with advanced chemometrics, it was possible to discriminate thyme from blossom honey and develop binary discriminatory models with high predictive power. In conclusion, a holistic approach to assessing the botanical origin of Greek honey is presented, highlighting the complementarity of the three applied metabolomic approaches.
U2 - 10.3390/molecules27144444
DO - 10.3390/molecules27144444
M3 - Article
C2 - 35889316
VL - 27
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 14
M1 - 4444
ER -