Transparent soil microcosms allow 3D spatial quantification of soil microbiological processes in vivo

Helen F. Downie, Tracy A. Valentine, Wilfred Otten, Andrew J. Spiers, Lionel X. Dupuy

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Abstract

The recently developed transparent soil consists of particles of Nafion, a polymer with a low refractive index (RI), which is prepared by milling and chemical treatment for use as a soil analogue. After the addition of a RI-matched solution, confocal imaging can be carried out in vivo and without destructive sampling. In a previous study, we showed that the new substrate provides a good approximation of plant growth conditions found in natural soils. In this paper, we present further development of the techniques for detailed quantitative analysis of images of root-microbe interactions in situ. Using this system it was possible for the first time to analyse bacterial distribution along the roots and in the bulk substrate in vivo. These findings indicate that the coupling of transparent soil with light microscopy is an important advance towards the discovery of the mechanisms of microbial colonisation of the rhizosphere.
Original languageEnglish
Article numbere970421
Number of pages4
JournalPlant Signaling & Behavior
Volume9
Issue number10
DOIs
Publication statusPublished - 1 Oct 2014

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microcosm
refractive index
soil
substrate
quantitative analysis
rhizosphere
microscopy
colonization
polymer
sampling

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Downie, Helen F. ; Valentine, Tracy A. ; Otten, Wilfred ; Spiers, Andrew J. ; Dupuy, Lionel X. / Transparent soil microcosms allow 3D spatial quantification of soil microbiological processes in vivo. In: Plant Signaling & Behavior. 2014 ; Vol. 9, No. 10.
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Transparent soil microcosms allow 3D spatial quantification of soil microbiological processes in vivo. / Downie, Helen F.; Valentine, Tracy A.; Otten, Wilfred; Spiers, Andrew J.; Dupuy, Lionel X.

In: Plant Signaling & Behavior, Vol. 9, No. 10, e970421, 01.10.2014.

Research output: Contribution to journalArticle

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