Tyrosine dephosphorylation underlies DHPG-induced LTD

Peter R. Moult, Rebecca Schnabel, Ian C. Kilpatrick, Zafar I. Bashir, Graham L. Collingridge

Research output: Contribution to journal › Article

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Abstract

A form of long-term depression (LTD) of synaptic transmission can be induced by bath application of the group I metabotropic glutamate (mGlu) receptor agonist (RS)-3,5-dihydroxyphenylglycine (DHPG). The mechanisms responsible for the induction and expression of DHPG-induced LTD in the CA1 region of the hippocampus are currently the subject of intense investigation. Here we show that two protein tyrosine kinase (PTK) inhibitors (10 μM lavendustin A or 30 μM genistein) have little effect on DHPG-induced LTD. In contrast two protein tyrosine phosphatase (PTP) inhibitors (1 mM orthovanadate or 15 μM phenyl–arsine oxide) significantly inhibited DHPG-induced LTD. These data suggest that DHPG-induced LTD involves activation of a protein tyrosine phosphatase.
Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalNeuropharmacology
Volume43
Issue number2
DOIs
Publication statusPublished - Aug 2002

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Tyrosine
Protein Tyrosine Phosphatases
Excitatory Amino Acid Agonists
Metabotropic Glutamate Receptors
Vanadates
Genistein
Protein Kinase Inhibitors
Baths
Synaptic Transmission
Protein-Tyrosine Kinases
Hippocampus
dihydroxyphenylethylene glycol

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Moult, P. R., Schnabel, R., Kilpatrick, I. C., Bashir, Z. I., & Collingridge, G. L. (2002). Tyrosine dephosphorylation underlies DHPG-induced LTD. Neuropharmacology, 43(2), 175-180. https://doi.org/10.1016/S0028-3908(02)00110-7
Moult, Peter R. ; Schnabel, Rebecca ; Kilpatrick, Ian C. ; Bashir, Zafar I. ; Collingridge, Graham L. / Tyrosine dephosphorylation underlies DHPG-induced LTD. In: Neuropharmacology. 2002 ; Vol. 43, No. 2. pp. 175-180.
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Moult, PR, Schnabel, R, Kilpatrick, IC, Bashir, ZI & Collingridge, GL 2002, 'Tyrosine dephosphorylation underlies DHPG-induced LTD', Neuropharmacology, vol. 43, no. 2, pp. 175-180. https://doi.org/10.1016/S0028-3908(02)00110-7

Tyrosine dephosphorylation underlies DHPG-induced LTD. / Moult, Peter R.; Schnabel, Rebecca; Kilpatrick, Ian C.; Bashir, Zafar I.; Collingridge, Graham L.

In: Neuropharmacology, Vol. 43, No. 2, 08.2002, p. 175-180.

Research output: Contribution to journal › Article

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AU - Collingridge, Graham L.

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AB - A form of long-term depression (LTD) of synaptic transmission can be induced by bath application of the group I metabotropic glutamate (mGlu) receptor agonist (RS)-3,5-dihydroxyphenylglycine (DHPG). The mechanisms responsible for the induction and expression of DHPG-induced LTD in the CA1 region of the hippocampus are currently the subject of intense investigation. Here we show that two protein tyrosine kinase (PTK) inhibitors (10 μM lavendustin A or 30 μM genistein) have little effect on DHPG-induced LTD. In contrast two protein tyrosine phosphatase (PTP) inhibitors (1 mM orthovanadate or 15 μM phenyl–arsine oxide) significantly inhibited DHPG-induced LTD. These data suggest that DHPG-induced LTD involves activation of a protein tyrosine phosphatase.

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JO - Neuropharmacology

JF - Neuropharmacology

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Moult PR, Schnabel R, Kilpatrick IC, Bashir ZI, Collingridge GL. Tyrosine dephosphorylation underlies DHPG-induced LTD. Neuropharmacology. 2002 Aug;43(2):175-180. https://doi.org/10.1016/S0028-3908(02)00110-7

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