Understanding oxygen management in grey seal blubber

Research output: Contribution to conferenceAbstract

Abstract

GThe ability to undergo dramatic changes in body mass and condition is vital for grey seal
survival. Biomedical studies suggest that oxygen availability in fat tissue is affected by tissue
expansion during obesity and is a potential health concern. However, whether and how
blubber remains normoxic under radical adiposity changes is unknown. Grey seals have a
similar fat percentage to obese humans; therefore, we hypothesise that oxygen availability in
blubber will be affected by increasing tissue depths and adiposity. For the first time, we
measured oxygen saturation (SpO2) and partial pressure of oxygen (pO2) on the dorsal
flank´s blubber of sedated and anaesthetised juvenile grey seals (n=12). Blood oxygen
saturation, heart rate and breathing rate were monitored throughout sampling. Oxygen
saturation and blood flow were recorded using non-invasive Near Infrared Spectroscopy
(NIRS) to assess oxygen supply. Simultaneously, dissolved oxygen availability was measured
in the same blubber region at different depths, with a non-consuming, temperature
compensated optical oxygen probe (NX-LAS-8/OT/E, Oxford optronix). In four cases, two
probes were inserted simultaneously 33±3 mm apart to examine inter-site variability. To
investigate the influence of adiposity, we estimated fatness by photogrammetry and measured
blubber depth using ultrasound. Furthermore, four animals were resampled after weight
increases of 6 to 25 Kg. Preliminary results suggest substantial decreases in blood SpO2
correspond with a lagged decrease in blubber pO2. Large inter-individual differences have
been recorded, however values are similar to those reported in human studies. Surprisingly,
pO2 varies irrespectively of blubber depth or adiposity. Further statistical analysis will better
evaluate adiposity effects and vascularity. Our data suggest that blubber is well is not hypoxic,
despite its large volume and radical changes in size. Further work on freely diving animals
needs to be undertaken to explore changes in oxygenation and potential hypoxia during large
changes in oxygen availability
Original languageEnglish
Pages16-16
Number of pages1
Publication statusPublished - Jan 2018

Fingerprint

Earless Seals
Oxygen
Adiposity
Photogrammetry
Fats
Diving
Aptitude
Near-Infrared Spectroscopy
Partial Pressure
Individuality
Respiration
Obesity
Heart Rate

Cite this

@conference{69286b3cf7d34291b24c03e0dfa44d90,
title = "Understanding oxygen management in grey seal blubber",
abstract = "GThe ability to undergo dramatic changes in body mass and condition is vital for grey sealsurvival. Biomedical studies suggest that oxygen availability in fat tissue is affected by tissueexpansion during obesity and is a potential health concern. However, whether and howblubber remains normoxic under radical adiposity changes is unknown. Grey seals have asimilar fat percentage to obese humans; therefore, we hypothesise that oxygen availability inblubber will be affected by increasing tissue depths and adiposity. For the first time, wemeasured oxygen saturation (SpO2) and partial pressure of oxygen (pO2) on the dorsalflank´s blubber of sedated and anaesthetised juvenile grey seals (n=12). Blood oxygensaturation, heart rate and breathing rate were monitored throughout sampling. Oxygensaturation and blood flow were recorded using non-invasive Near Infrared Spectroscopy(NIRS) to assess oxygen supply. Simultaneously, dissolved oxygen availability was measuredin the same blubber region at different depths, with a non-consuming, temperaturecompensated optical oxygen probe (NX-LAS-8/OT/E, Oxford optronix). In four cases, twoprobes were inserted simultaneously 33±3 mm apart to examine inter-site variability. Toinvestigate the influence of adiposity, we estimated fatness by photogrammetry and measuredblubber depth using ultrasound. Furthermore, four animals were resampled after weightincreases of 6 to 25 Kg. Preliminary results suggest substantial decreases in blood SpO2correspond with a lagged decrease in blubber pO2. Large inter-individual differences havebeen recorded, however values are similar to those reported in human studies. Surprisingly,pO2 varies irrespectively of blubber depth or adiposity. Further statistical analysis will betterevaluate adiposity effects and vascularity. Our data suggest that blubber is well is not hypoxic,despite its large volume and radical changes in size. Further work on freely diving animalsneeds to be undertaken to explore changes in oxygenation and potential hypoxia during largechanges in oxygen availability",
author = "{Oller Lopez}, Laura and Joel Rocha and Chris McKnight and Bennett, {Kimberley A.}",
year = "2018",
month = "1",
language = "English",
pages = "16--16",

}

Understanding oxygen management in grey seal blubber. / Oller Lopez, Laura; Rocha, Joel; McKnight, Chris; Bennett, Kimberley A. .

2018. 16-16.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Understanding oxygen management in grey seal blubber

AU - Oller Lopez, Laura

AU - Rocha, Joel

AU - McKnight, Chris

AU - Bennett, Kimberley A.

PY - 2018/1

Y1 - 2018/1

N2 - GThe ability to undergo dramatic changes in body mass and condition is vital for grey sealsurvival. Biomedical studies suggest that oxygen availability in fat tissue is affected by tissueexpansion during obesity and is a potential health concern. However, whether and howblubber remains normoxic under radical adiposity changes is unknown. Grey seals have asimilar fat percentage to obese humans; therefore, we hypothesise that oxygen availability inblubber will be affected by increasing tissue depths and adiposity. For the first time, wemeasured oxygen saturation (SpO2) and partial pressure of oxygen (pO2) on the dorsalflank´s blubber of sedated and anaesthetised juvenile grey seals (n=12). Blood oxygensaturation, heart rate and breathing rate were monitored throughout sampling. Oxygensaturation and blood flow were recorded using non-invasive Near Infrared Spectroscopy(NIRS) to assess oxygen supply. Simultaneously, dissolved oxygen availability was measuredin the same blubber region at different depths, with a non-consuming, temperaturecompensated optical oxygen probe (NX-LAS-8/OT/E, Oxford optronix). In four cases, twoprobes were inserted simultaneously 33±3 mm apart to examine inter-site variability. Toinvestigate the influence of adiposity, we estimated fatness by photogrammetry and measuredblubber depth using ultrasound. Furthermore, four animals were resampled after weightincreases of 6 to 25 Kg. Preliminary results suggest substantial decreases in blood SpO2correspond with a lagged decrease in blubber pO2. Large inter-individual differences havebeen recorded, however values are similar to those reported in human studies. Surprisingly,pO2 varies irrespectively of blubber depth or adiposity. Further statistical analysis will betterevaluate adiposity effects and vascularity. Our data suggest that blubber is well is not hypoxic,despite its large volume and radical changes in size. Further work on freely diving animalsneeds to be undertaken to explore changes in oxygenation and potential hypoxia during largechanges in oxygen availability

AB - GThe ability to undergo dramatic changes in body mass and condition is vital for grey sealsurvival. Biomedical studies suggest that oxygen availability in fat tissue is affected by tissueexpansion during obesity and is a potential health concern. However, whether and howblubber remains normoxic under radical adiposity changes is unknown. Grey seals have asimilar fat percentage to obese humans; therefore, we hypothesise that oxygen availability inblubber will be affected by increasing tissue depths and adiposity. For the first time, wemeasured oxygen saturation (SpO2) and partial pressure of oxygen (pO2) on the dorsalflank´s blubber of sedated and anaesthetised juvenile grey seals (n=12). Blood oxygensaturation, heart rate and breathing rate were monitored throughout sampling. Oxygensaturation and blood flow were recorded using non-invasive Near Infrared Spectroscopy(NIRS) to assess oxygen supply. Simultaneously, dissolved oxygen availability was measuredin the same blubber region at different depths, with a non-consuming, temperaturecompensated optical oxygen probe (NX-LAS-8/OT/E, Oxford optronix). In four cases, twoprobes were inserted simultaneously 33±3 mm apart to examine inter-site variability. Toinvestigate the influence of adiposity, we estimated fatness by photogrammetry and measuredblubber depth using ultrasound. Furthermore, four animals were resampled after weightincreases of 6 to 25 Kg. Preliminary results suggest substantial decreases in blood SpO2correspond with a lagged decrease in blubber pO2. Large inter-individual differences havebeen recorded, however values are similar to those reported in human studies. Surprisingly,pO2 varies irrespectively of blubber depth or adiposity. Further statistical analysis will betterevaluate adiposity effects and vascularity. Our data suggest that blubber is well is not hypoxic,despite its large volume and radical changes in size. Further work on freely diving animalsneeds to be undertaken to explore changes in oxygenation and potential hypoxia during largechanges in oxygen availability

M3 - Abstract

SP - 16

EP - 16

ER -