Use of time-resolved fluorescence to monitor bioactive compounds in plant based foodstuffs

M. Adilia Lemos, Katarína Sárniková, Francesca Bot, Monica Anese, Graham Hungerford

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)
    115 Downloads (Pure)

    Abstract

    The study of compounds that exhibit antioxidant activity has recently received much interest in the food industry because of their potential health benefits. Most of these compounds are plant based, such as polyphenolics and carotenoids, and there is a need to monitor them from the field through processing and into the body. Ideally, a monitoring technique should be non-invasive with the potential for remote capabilities. The application of the phenomenon of fluorescence has proved to be well suited, as many plant associated compounds exhibit fluorescence. The photophysical behaviour of fluorescent molecules is also highly dependent on their microenvironment, making them suitable probes to monitor changes in pH, viscosity and polarity, for example. Time-resolved fluorescence techniques have recently come to the fore, as they offer the ability to obtain more information, coupled with the fact that the fluorescence lifetime is an absolute measure, while steady state just provides relative and average information. In this work, we will present illustrative time-resolved measurements, rather than a comprehensive review, to show the potential of time-resolved fluorescence applied to the study of bioactive substances. The aim is to help assess if any changes occur in their form, going from extraction via storage and cooking to the interaction with serum albumin, a principal blood transport protein.
    Original languageEnglish
    Pages (from-to)367-397
    Number of pages31
    JournalBiosensors
    Volume5
    Issue number3
    DOIs
    Publication statusPublished - 26 Jun 2015

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