Using blubber explants to investigate adipose function in grey seals: glycolytic, lipolytic and gene expression responses to glucose and hydrocortisone

Kimberley A. Bennett*, Kelly J. Robinson, Simon E. W. Moss, Sebastian Millward, Ailsa J. Hall

*Corresponding author for this work

    Research output: Contribution to journalArticle

    2 Citations (Scopus)
    57 Downloads (Pure)

    Abstract

    Adipose tissue is fundamental to energy balance, which underpins fitness and survival. Knowledge of adipose regulation in animals that undergo rapid fat deposition and mobilisation aids understanding of their energetic responses to rapid environmental change. Tissue explants can be used to investigate adipose regulation in wildlife species with large fat reserves, when opportunities for organismal experimental work are limited. We investigated glucose removal, lactate, glycerol and NEFA accumulation in media, and metabolic gene expression in blubber explants from wild grey seals. Glycolysis was higher in explants incubated in 25 mM glucose (HG) for 24 h compared to controls (C: 5.5 mM glucose). Adipose-derived lactate likely contributes to high endogenous glucose production in seals. Lipolysis was not stimulated by HG or high hydrocortisone (HC: 500 nM hydrocortisone) and was lower in heavier animals. HC caused NEFA accumulation in media to decrease by ~30% relative to C in females, indicative of increased lipogenesis. Lipolysis was higher in males than females in C and HG conditions. Lower relative abundance of 11-β-hydroxysteroid dehydrogenase 1 mRNA in HG explants suggests glucose involvement in blubber cortisol sensitivity. Our findings can help predict energy balance responses to stress and nutritional state in seals, and highlight the use of explants to study fat tissue function in wildlife.
    Original languageEnglish
    Article number7731
    Number of pages11
    JournalScientific Reports
    Volume7
    Early online date10 Aug 2017
    DOIs
    Publication statusPublished - 10 Aug 2017

    Fingerprint

    blubber
    seals
    cortisol
    explants
    gene expression
    glucose
    lipolysis
    energy balance
    lactates
    free fatty acids
    lipids
    animal law
    fish and wildlife law
    hydroxysteroid dehydrogenases
    lipogenesis
    glycolysis
    adipose tissue
    nutritional status
    glycerol
    stress response

    Cite this

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    title = "Using blubber explants to investigate adipose function in grey seals: glycolytic, lipolytic and gene expression responses to glucose and hydrocortisone",
    abstract = "Adipose tissue is fundamental to energy balance, which underpins fitness and survival. Knowledge of adipose regulation in animals that undergo rapid fat deposition and mobilisation aids understanding of their energetic responses to rapid environmental change. Tissue explants can be used to investigate adipose regulation in wildlife species with large fat reserves, when opportunities for organismal experimental work are limited. We investigated glucose removal, lactate, glycerol and NEFA accumulation in media, and metabolic gene expression in blubber explants from wild grey seals. Glycolysis was higher in explants incubated in 25 mM glucose (HG) for 24 h compared to controls (C: 5.5 mM glucose). Adipose-derived lactate likely contributes to high endogenous glucose production in seals. Lipolysis was not stimulated by HG or high hydrocortisone (HC: 500 nM hydrocortisone) and was lower in heavier animals. HC caused NEFA accumulation in media to decrease by ~30{\%} relative to C in females, indicative of increased lipogenesis. Lipolysis was higher in males than females in C and HG conditions. Lower relative abundance of 11-β-hydroxysteroid dehydrogenase 1 mRNA in HG explants suggests glucose involvement in blubber cortisol sensitivity. Our findings can help predict energy balance responses to stress and nutritional state in seals, and highlight the use of explants to study fat tissue function in wildlife.",
    author = "Bennett, {Kimberley A.} and Robinson, {Kelly J.} and Moss, {Simon E. W.} and Sebastian Millward and Hall, {Ailsa J.}",
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    Using blubber explants to investigate adipose function in grey seals : glycolytic, lipolytic and gene expression responses to glucose and hydrocortisone. / Bennett, Kimberley A.; Robinson, Kelly J.; Moss, Simon E. W.; Millward, Sebastian; Hall, Ailsa J.

    In: Scientific Reports, Vol. 7, 7731, 10.08.2017.

    Research output: Contribution to journalArticle

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    AU - Robinson, Kelly J.

    AU - Moss, Simon E. W.

    AU - Millward, Sebastian

    AU - Hall, Ailsa J.

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